S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potenti...
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Sociedade Brasileira de Pesquisa Odontológica
2010-06-01
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doaj-614f1b1dad634ec088e977dbc86037592020-11-24T23:36:24ZengSociedade Brasileira de Pesquisa Odontológica Brazilian Oral Research1806-83242010-06-0124213514110.1590/S1806-83242010000200002S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralizationRenzo Alberto Ccahuana-VásquezJaime Aparecido CuryThe aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37°C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002Streptococcus mutansBiofilmsChlorhexidineSucroseDental caries |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Renzo Alberto Ccahuana-Vásquez Jaime Aparecido Cury |
spellingShingle |
Renzo Alberto Ccahuana-Vásquez Jaime Aparecido Cury S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization Brazilian Oral Research Streptococcus mutans Biofilms Chlorhexidine Sucrose Dental caries |
author_facet |
Renzo Alberto Ccahuana-Vásquez Jaime Aparecido Cury |
author_sort |
Renzo Alberto Ccahuana-Vásquez |
title |
S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization |
title_short |
S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization |
title_full |
S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization |
title_fullStr |
S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization |
title_full_unstemmed |
S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization |
title_sort |
s. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization |
publisher |
Sociedade Brasileira de Pesquisa Odontológica |
series |
Brazilian Oral Research |
issn |
1806-8324 |
publishDate |
2010-06-01 |
description |
The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37°C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization. |
topic |
Streptococcus mutans Biofilms Chlorhexidine Sucrose Dental caries |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002 |
work_keys_str_mv |
AT renzoalbertoccahuanavasquez smutansbiofilmmodeltoevaluateantimicrobialsubstancesandenameldemineralization AT jaimeaparecidocury smutansbiofilmmodeltoevaluateantimicrobialsubstancesandenameldemineralization |
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