S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization

The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potenti...

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Main Authors: Renzo Alberto Ccahuana-Vásquez, Jaime Aparecido Cury
Format: Article
Language:English
Published: Sociedade Brasileira de Pesquisa Odontológica 2010-06-01
Series:Brazilian Oral Research
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002
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spelling doaj-614f1b1dad634ec088e977dbc86037592020-11-24T23:36:24ZengSociedade Brasileira de Pesquisa Odontológica Brazilian Oral Research1806-83242010-06-0124213514110.1590/S1806-83242010000200002S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralizationRenzo Alberto Ccahuana-VásquezJaime Aparecido CuryThe aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37°C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002Streptococcus mutansBiofilmsChlorhexidineSucroseDental caries
collection DOAJ
language English
format Article
sources DOAJ
author Renzo Alberto Ccahuana-Vásquez
Jaime Aparecido Cury
spellingShingle Renzo Alberto Ccahuana-Vásquez
Jaime Aparecido Cury
S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
Brazilian Oral Research
Streptococcus mutans
Biofilms
Chlorhexidine
Sucrose
Dental caries
author_facet Renzo Alberto Ccahuana-Vásquez
Jaime Aparecido Cury
author_sort Renzo Alberto Ccahuana-Vásquez
title S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_short S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_full S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_fullStr S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_full_unstemmed S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
title_sort s. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization
publisher Sociedade Brasileira de Pesquisa Odontológica
series Brazilian Oral Research
issn 1806-8324
publishDate 2010-06-01
description The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37°C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.
topic Streptococcus mutans
Biofilms
Chlorhexidine
Sucrose
Dental caries
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000200002
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AT jaimeaparecidocury smutansbiofilmmodeltoevaluateantimicrobialsubstancesandenameldemineralization
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