LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffei
Talaromyces marneffei (TM) is an important opportunistic pathogenic fungus capable of causing disseminated lethal infection. In our previous study, we identified host lncRNAs and mRNAs that are dysregulated in TM-infected bronchial epithelial cells. In this report, we verified that IL-6, a key facto...
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doaj-623b92d2f61e47909edc3bbe1c52d3ff2020-11-25T02:55:45ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-01-011010.3389/fimmu.2019.02977479179LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffeiYinghua Li0Huan Chen1Shuyi Li2Yu Li3Guangnan Liu4Jing Bai5Honglin Luo6Xiuwan Lan7Zhiyi He8Department of Pulmonary and Critical Care Medicine, Second Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pulmonary and Critical Care Medicine, Sixth Affiliated Hospital of Guangxi Medical University, Yulin, ChinaGuangxi Colleges and Universities Key Laboratory of Preclinical Medicine Research, Guangxi Medical University, Nanning, ChinaDepartment of Pulmonary and Critical Care Medicine, Second Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pulmonary and Critical Care Medicine, Second Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pulmonary and Critical Care Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaSchool of Basic Medicine, Guangxi Medical University, Nanning, ChinaGuangxi Colleges and Universities Key Laboratory of Preclinical Medicine Research, Guangxi Medical University, Nanning, ChinaDepartment of Pulmonary and Critical Care Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaTalaromyces marneffei (TM) is an important opportunistic pathogenic fungus capable of causing disseminated lethal infection. In our previous study, we identified host lncRNAs and mRNAs that are dysregulated in TM-infected bronchial epithelial cells. In this report, we verified that IL-6, a key factor in acute inflammatory response, is down-regulated in TM pathogenesis. To elucidate the mechanism of IL-6 regulation, we analyzed the coding/non-coding network, and identified lncSSBP1, a novel lncRNA that is up-regulated by TM. Our results demonstrate that overexpression of lncSSBP1 decreases IL-6 mRNA expression, whereas knockdown of lncSSBP1 enhances IL-6 mRNA expression. Though lncSSBP1 is primarily localized to the nucleus, bioinformatics analysis suggests that it is unlikely to function as competing endogenous RNA or to interact with IL-6 transcription factors. Instead, RNA pull down and RNA immunoprecipitation assays showed that lncSSBP1 binds specifically to heterogenous nuclear ribonucleoprotein K (hnRNPK), which is involved in IL-6 mRNA processing. Our findings suggest that lncSSBP1 may affect IL-6 mRNA expression during TM infection through interaction with hnRNPk in bronchial epithelial cells. Our results suggest a novel pathway by which TM may suppress the immune response to its advantage.https://www.frontiersin.org/article/10.3389/fimmu.2019.02977/fullmicrobial immunologyfungusTalaromyces marneffeilncRNAIL-6hnRNPK |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yinghua Li Huan Chen Shuyi Li Yu Li Guangnan Liu Jing Bai Honglin Luo Xiuwan Lan Zhiyi He |
spellingShingle |
Yinghua Li Huan Chen Shuyi Li Yu Li Guangnan Liu Jing Bai Honglin Luo Xiuwan Lan Zhiyi He LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffei Frontiers in Immunology microbial immunology fungus Talaromyces marneffei lncRNA IL-6 hnRNPK |
author_facet |
Yinghua Li Huan Chen Shuyi Li Yu Li Guangnan Liu Jing Bai Honglin Luo Xiuwan Lan Zhiyi He |
author_sort |
Yinghua Li |
title |
LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffei |
title_short |
LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffei |
title_full |
LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffei |
title_fullStr |
LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffei |
title_full_unstemmed |
LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffei |
title_sort |
lncssbp1 functions as a negative regulator of il-6 through interaction with hnrnpk in bronchial epithelial cells infected with talaromyces marneffei |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2020-01-01 |
description |
Talaromyces marneffei (TM) is an important opportunistic pathogenic fungus capable of causing disseminated lethal infection. In our previous study, we identified host lncRNAs and mRNAs that are dysregulated in TM-infected bronchial epithelial cells. In this report, we verified that IL-6, a key factor in acute inflammatory response, is down-regulated in TM pathogenesis. To elucidate the mechanism of IL-6 regulation, we analyzed the coding/non-coding network, and identified lncSSBP1, a novel lncRNA that is up-regulated by TM. Our results demonstrate that overexpression of lncSSBP1 decreases IL-6 mRNA expression, whereas knockdown of lncSSBP1 enhances IL-6 mRNA expression. Though lncSSBP1 is primarily localized to the nucleus, bioinformatics analysis suggests that it is unlikely to function as competing endogenous RNA or to interact with IL-6 transcription factors. Instead, RNA pull down and RNA immunoprecipitation assays showed that lncSSBP1 binds specifically to heterogenous nuclear ribonucleoprotein K (hnRNPK), which is involved in IL-6 mRNA processing. Our findings suggest that lncSSBP1 may affect IL-6 mRNA expression during TM infection through interaction with hnRNPk in bronchial epithelial cells. Our results suggest a novel pathway by which TM may suppress the immune response to its advantage. |
topic |
microbial immunology fungus Talaromyces marneffei lncRNA IL-6 hnRNPK |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.02977/full |
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