Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes
Cryo-electron microscopy (cryo-EM) has for a long time been a technique of choice for determining structure of large and flexible macromolecular complexes that were difficult to study by other experimental techniques such as X-ray crystallography or nuclear magnetic resonance. However, a fast develo...
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doaj-6279b58933914ba3972f57d9fc0d6a892020-11-25T00:30:58ZengElsevierComputational and Structural Biotechnology Journal2001-03702016-01-0114C38539010.1016/j.csbj.2016.10.002Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular ComplexesSlavica JonićCryo-electron microscopy (cryo-EM) has for a long time been a technique of choice for determining structure of large and flexible macromolecular complexes that were difficult to study by other experimental techniques such as X-ray crystallography or nuclear magnetic resonance. However, a fast development of instruments and software for cryo-EM in the last decade has allowed that a large range of complexes can be studied by cryo-EM, and that their structures can be obtained at near-atomic resolution, including the structures of small complexes (e.g., membrane proteins) whose size was earlier an obstacle to cryo-EM. Image analysis to identify multiple coexisting structures in the same specimen (multiconformation reconstruction) is now routinely done both to solve structures at near-atomic resolution and to study conformational dynamics. Methods for multiconformation reconstruction and latest examples of their applications are the focus of this review.http://www.sciencedirect.com/science/article/pii/S2001037016300551Cryo-electron microscopySingle particle analysisMacromolecular complexesHeterogeneityStructureFlexibilityConformational changesDynamics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Slavica Jonić |
spellingShingle |
Slavica Jonić Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes Computational and Structural Biotechnology Journal Cryo-electron microscopy Single particle analysis Macromolecular complexes Heterogeneity Structure Flexibility Conformational changes Dynamics |
author_facet |
Slavica Jonić |
author_sort |
Slavica Jonić |
title |
Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes |
title_short |
Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes |
title_full |
Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes |
title_fullStr |
Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes |
title_full_unstemmed |
Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes |
title_sort |
cryo-electron microscopy analysis of structurally heterogeneous macromolecular complexes |
publisher |
Elsevier |
series |
Computational and Structural Biotechnology Journal |
issn |
2001-0370 |
publishDate |
2016-01-01 |
description |
Cryo-electron microscopy (cryo-EM) has for a long time been a technique of choice for determining structure of large and flexible macromolecular complexes that were difficult to study by other experimental techniques such as X-ray crystallography or nuclear magnetic resonance. However, a fast development of instruments and software for cryo-EM in the last decade has allowed that a large range of complexes can be studied by cryo-EM, and that their structures can be obtained at near-atomic resolution, including the structures of small complexes (e.g., membrane proteins) whose size was earlier an obstacle to cryo-EM. Image analysis to identify multiple coexisting structures in the same specimen (multiconformation reconstruction) is now routinely done both to solve structures at near-atomic resolution and to study conformational dynamics. Methods for multiconformation reconstruction and latest examples of their applications are the focus of this review. |
topic |
Cryo-electron microscopy Single particle analysis Macromolecular complexes Heterogeneity Structure Flexibility Conformational changes Dynamics |
url |
http://www.sciencedirect.com/science/article/pii/S2001037016300551 |
work_keys_str_mv |
AT slavicajonic cryoelectronmicroscopyanalysisofstructurallyheterogeneousmacromolecularcomplexes |
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