Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv.
The development of megasporocytes and the functional megaspore formation in Deschampsia antarctica were analyzed with the use of microscopic methods. A single archesporial cell was formed directly under the epidermis in the micropylar region of the ovule without producing a parietal cell. In success...
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Polish Academy of Sciences
2016-06-01
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doaj-63f873ce73514bcc85fc1ad732b5ad7a2020-11-25T03:22:58ZengPolish Academy of SciencesPolish Polar Research2081-82622016-06-0137228930210.1515/popore-2016-0016popore-2016-0016Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv.Domaciuk Marcin0Leszczuk Agata1Szczuka Ewa2Kellmann-Sopyła Wioleta3Koc Justyna4Giełwanowska Irena Zakład Anatomii i Cytologii Roślin, Wydział Biologii i Biotechnologii, Uniwersytet Marii Curie Skłodowskiej, ul. Akademicka 19, 20-031 Lublin, Poland Zakład Anatomii i Cytologii Roślin, Wydział Biologii i Biotechnologii, Uniwersytet Marii Curie Skłodowskiej, ul. Akademicka 19, 20-031 Lublin, Poland Zakład Anatomii i Cytologii Roślin, Wydział Biologii i Biotechnologii, Uniwersytet Marii Curie Skłodowskiej, ul. Akademicka 19, 20-031 Lublin, Poland Katedra Fizjologii, Genetyki i Biotechnologii Roślin, Wydział Biologii i Biotechnologii, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul. Oczapowskiego 1A, 10-719 Olsztyn, Poland Katedra Fizjologii, Genetyki i Biotechnologii Roślin, Wydział Biologii i Biotechnologii, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul. Oczapowskiego 1A, 10-719 Olsztyn, PolandThe development of megasporocytes and the functional megaspore formation in Deschampsia antarctica were analyzed with the use of microscopic methods. A single archesporial cell was formed directly under the epidermis in the micropylar region of the ovule without producing a parietal cell. In successive stages of development, the meiocyte was transformed into a megaspore tetrad after meiosis. Most megaspores were arranged in a linear fashion, but some tetrads were T-shaped. Only one of the 60 analyzed ovules contained a cell in the direct proximity of the megasporocyte, which could be an aposporous initial. Most of the evaluated D. antarctica ovules featured monosporic embryo sacs of the Polygonum type. Approximately 30% of ovules contained numerous megaspores that were enlarged. The megaspores were located at chalazal and micropylar poles, and some ovules featured two megaspores – terminal and medial – in the chalazal region, or even three megaspores at the chalazal pole. In those cases, the micropylar megaspore was significantly smaller than the remaining megaspores, and it did not have the characteristic features of functional megaspores. Meiocytes and megaspores of D. antarctica contained polysaccharides that were detectable by PAS-reaction and aniline blue staining. Starch granules and cell walls of megasporocytes, megaspores and nucellar cells were PAS-positive. Fluorescent callose deposits were identified in the micropylar end of the megasporocytes. During meiosis and after its completion, thick callose deposits were also visible in the periclinal walls and in a small amount in the anticlinal walls of megaspores forming linear and T-shaped tetrads. Callose deposits fluorescence was not observed in the walls of the nucellar cells.http://www.degruyter.com/view/j/popore.2016.37.issue-2/popore-2016-0016/popore-2016-0016.xml?format=INTAntarcticDeschampsia antarcticamegasporocytemeiosismegaspore tetradcallose |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Domaciuk Marcin Leszczuk Agata Szczuka Ewa Kellmann-Sopyła Wioleta Koc Justyna Giełwanowska Irena |
spellingShingle |
Domaciuk Marcin Leszczuk Agata Szczuka Ewa Kellmann-Sopyła Wioleta Koc Justyna Giełwanowska Irena Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv. Polish Polar Research Antarctic Deschampsia antarctica megasporocyte meiosis megaspore tetrad callose |
author_facet |
Domaciuk Marcin Leszczuk Agata Szczuka Ewa Kellmann-Sopyła Wioleta Koc Justyna Giełwanowska Irena |
author_sort |
Domaciuk Marcin |
title |
Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv. |
title_short |
Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv. |
title_full |
Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv. |
title_fullStr |
Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv. |
title_full_unstemmed |
Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv. |
title_sort |
female sporogenesis in the native antarctic grass deschampsia antarctica desv. |
publisher |
Polish Academy of Sciences |
series |
Polish Polar Research |
issn |
2081-8262 |
publishDate |
2016-06-01 |
description |
The development of megasporocytes and the functional megaspore formation in Deschampsia antarctica were analyzed with the use of microscopic methods. A single archesporial cell was formed directly under the epidermis in the micropylar region of the ovule without producing a parietal cell. In successive stages of development, the meiocyte was transformed into a megaspore tetrad after meiosis. Most megaspores were arranged in a linear fashion, but some tetrads were T-shaped. Only one of the 60 analyzed ovules contained a cell in the direct proximity of the megasporocyte, which could be an aposporous initial. Most of the evaluated D. antarctica ovules featured monosporic embryo sacs of the Polygonum type. Approximately 30% of ovules contained numerous megaspores that were enlarged. The megaspores were located at chalazal and micropylar poles, and some ovules featured two megaspores – terminal and medial – in the chalazal region, or even three megaspores at the chalazal pole. In those cases, the micropylar megaspore was significantly smaller than the remaining megaspores, and it did not have the characteristic features of functional megaspores. Meiocytes and megaspores of D. antarctica contained polysaccharides that were detectable by PAS-reaction and aniline blue staining. Starch granules and cell walls of megasporocytes, megaspores and nucellar cells were PAS-positive. Fluorescent callose deposits were identified in the micropylar end of the megasporocytes. During meiosis and after its completion, thick callose deposits were also visible in the periclinal walls and in a small amount in the anticlinal walls of megaspores forming linear and T-shaped tetrads. Callose deposits fluorescence was not observed in the walls of the nucellar cells. |
topic |
Antarctic Deschampsia antarctica megasporocyte meiosis megaspore tetrad callose |
url |
http://www.degruyter.com/view/j/popore.2016.37.issue-2/popore-2016-0016/popore-2016-0016.xml?format=INT |
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