Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research

Organ-like cell clusters, so-called organoids, which exhibit self-organized and similar organ functionality as the tissue of origin, have provided a whole new level of bioinspiration for ex vivo systems. Microfluidic organoid or organs-on-a-chip platforms are a new group of micro-engineered promisin...

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Main Authors: Fahriye Duzagac, Gloria Saorin, Lorenzo Memeo, Vincenzo Canzonieri, Flavio Rizzolio
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Cancers
Subjects:
Online Access:https://www.mdpi.com/2072-6694/13/4/737
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spelling doaj-641354b8325549d6aec22cd2d41553a72021-02-11T00:04:56ZengMDPI AGCancers2072-66942021-02-011373773710.3390/cancers13040737Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer ResearchFahriye Duzagac0Gloria Saorin1Lorenzo Memeo2Vincenzo Canzonieri3Flavio Rizzolio4Department of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, 30123 Venezia, ItalyDepartment of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, 30123 Venezia, ItalyDepartment of Experimental Oncology, Mediterranean Institute of Oncology (IOM), 95029 Catania, ItalyPathology Unit, Centro di Riferimento Oncologico di Aviano (CRO) IRCCS, 33081 Aviano, ItalyDepartment of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, 30123 Venezia, ItalyOrgan-like cell clusters, so-called organoids, which exhibit self-organized and similar organ functionality as the tissue of origin, have provided a whole new level of bioinspiration for ex vivo systems. Microfluidic organoid or organs-on-a-chip platforms are a new group of micro-engineered promising models that recapitulate 3D tissue structure and physiology and combines several advantages of current in vivo and in vitro models. Microfluidics technology is used in numerous applications since it allows us to control and manipulate fluid flows with a high degree of accuracy. This system is an emerging tool for understanding disease development and progression, especially for personalized therapeutic strategies for cancer treatment, which provide well-grounded, cost-effective, powerful, fast, and reproducible results. In this review, we highlight how the organoid-on-a-chip models have improved the potential of efficiency and reproducibility of organoid cultures. More widely, we discuss current challenges and development on organoid culture systems together with microfluidic approaches and their limitations. Finally, we describe the recent progress and potential utilization in the organs-on-a-chip practice.https://www.mdpi.com/2072-6694/13/4/737organoidstumoroidsmicrofluidicsorgans-on-a-chipliving biobanks
collection DOAJ
language English
format Article
sources DOAJ
author Fahriye Duzagac
Gloria Saorin
Lorenzo Memeo
Vincenzo Canzonieri
Flavio Rizzolio
spellingShingle Fahriye Duzagac
Gloria Saorin
Lorenzo Memeo
Vincenzo Canzonieri
Flavio Rizzolio
Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research
Cancers
organoids
tumoroids
microfluidics
organs-on-a-chip
living biobanks
author_facet Fahriye Duzagac
Gloria Saorin
Lorenzo Memeo
Vincenzo Canzonieri
Flavio Rizzolio
author_sort Fahriye Duzagac
title Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research
title_short Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research
title_full Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research
title_fullStr Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research
title_full_unstemmed Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research
title_sort microfluidic organoids-on-a-chip: quantum leap in cancer research
publisher MDPI AG
series Cancers
issn 2072-6694
publishDate 2021-02-01
description Organ-like cell clusters, so-called organoids, which exhibit self-organized and similar organ functionality as the tissue of origin, have provided a whole new level of bioinspiration for ex vivo systems. Microfluidic organoid or organs-on-a-chip platforms are a new group of micro-engineered promising models that recapitulate 3D tissue structure and physiology and combines several advantages of current in vivo and in vitro models. Microfluidics technology is used in numerous applications since it allows us to control and manipulate fluid flows with a high degree of accuracy. This system is an emerging tool for understanding disease development and progression, especially for personalized therapeutic strategies for cancer treatment, which provide well-grounded, cost-effective, powerful, fast, and reproducible results. In this review, we highlight how the organoid-on-a-chip models have improved the potential of efficiency and reproducibility of organoid cultures. More widely, we discuss current challenges and development on organoid culture systems together with microfluidic approaches and their limitations. Finally, we describe the recent progress and potential utilization in the organs-on-a-chip practice.
topic organoids
tumoroids
microfluidics
organs-on-a-chip
living biobanks
url https://www.mdpi.com/2072-6694/13/4/737
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AT vincenzocanzonieri microfluidicorganoidsonachipquantumleapincancerresearch
AT flaviorizzolio microfluidicorganoidsonachipquantumleapincancerresearch
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