Using steered molecular dynamics to predict and assess Hsp70 substrate-binding domain mutants that alter prion propagation.
Genetic screens using Saccharomyces cerevisiae have identified an array of cytosolic Hsp70 mutants that are impaired in the ability to propagate the yeast [PSI(+)] prion. The best characterized of these mutants is the Ssa1 L483W mutant (so-called SSA1-21), which is located in the substrate-binding d...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS Computational Biology |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23382668/pdf/?tool=EBI |