Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma
Introduction: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinar...
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doaj-6592637be6d14ab784887024d651e72f2021-02-03T06:23:09ZengWolters Kluwer Medknow PublicationsIndian Journal of Urology0970-15911998-38242021-01-01371596410.4103/iju.IJU_330_20Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinomaAbhilash CheriyanArun Jose NellickalNirmal Thampi JohnLakshmanan JeyaseelanSantosh KumarAntony DevasiaNitin KekreIntroduction: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinary aquaporin-1 (uAQP-1), an upcoming urinary biomarker, for renal cell carcinoma. Materials and Methods: In this prospective biomarker study, urine samples were obtained preoperatively from 36 patients with an imaged renal mass suggestive of RCC and 24 healthy age-matched controls, chosen from among voluntary kidney donors. uAQP-1 concentrations were estimated with a sensitive and specific enzyme-linked immunosorbent assay (ELISA) and normalized by estimation of urinary creatinine. The Mann–Whitney U-test was used to compare differences between any two groups. A receiver operator characteristic (ROC) curve was plotted to analyze the diagnostic accuracy of uAQP-1 for RCC. Results: The median uAQP-1 concentration among the cases and controls was 8.78 ng/mg creatinine (interquartile range [IQR]: 5.56–12.67) and 9.52 ng/mg creatinine (IQR: 5.55–12.45), respectively. There was no significant difference in uAQP-1 concentrations between the two groups. ROC analysis showed that, for a cutoff value of 8 ng/mg creatinine, the sensitivity and specificity of uAQP-1 as a diagnostic test were 47.2% and 66.7%, respectively, and area under the curve was 0.52 (95% confidence interval: 0.42–0.62). Conclusions: uAQP-1 concentrations did not discriminate between healthy individuals and patients with RCC. The results of this study suggest that uAQP-1 may not be a suitable diagnostic biomarker for RCC in the study population.http://www.indianjurol.com/article.asp?issn=0970-1591;year=2021;volume=37;issue=1;spage=59;epage=64;aulast=Cheriyan |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abhilash Cheriyan Arun Jose Nellickal Nirmal Thampi John Lakshmanan Jeyaseelan Santosh Kumar Antony Devasia Nitin Kekre |
spellingShingle |
Abhilash Cheriyan Arun Jose Nellickal Nirmal Thampi John Lakshmanan Jeyaseelan Santosh Kumar Antony Devasia Nitin Kekre Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma Indian Journal of Urology |
author_facet |
Abhilash Cheriyan Arun Jose Nellickal Nirmal Thampi John Lakshmanan Jeyaseelan Santosh Kumar Antony Devasia Nitin Kekre |
author_sort |
Abhilash Cheriyan |
title |
Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_short |
Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_full |
Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_fullStr |
Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_full_unstemmed |
Diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
title_sort |
diagnostic accuracy of urinary aquaporin-1 as a biomarker for renal cell carcinoma |
publisher |
Wolters Kluwer Medknow Publications |
series |
Indian Journal of Urology |
issn |
0970-1591 1998-3824 |
publishDate |
2021-01-01 |
description |
Introduction: Optimal patient selection plays a vital role in management of renal tumors with the introduction of nephron-sparing approaches and active surveillance. A reliable and accurate diagnostic biomarker will be a useful adjunct to decision-making. We studied the diagnostic accuracy of urinary aquaporin-1 (uAQP-1), an upcoming urinary biomarker, for renal cell carcinoma.
Materials and Methods: In this prospective biomarker study, urine samples were obtained preoperatively from 36 patients with an imaged renal mass suggestive of RCC and 24 healthy age-matched controls, chosen from among voluntary kidney donors. uAQP-1 concentrations were estimated with a sensitive and specific enzyme-linked immunosorbent assay (ELISA) and normalized by estimation of urinary creatinine. The Mann–Whitney U-test was used to compare differences between any two groups. A receiver operator characteristic (ROC) curve was plotted to analyze the diagnostic accuracy of uAQP-1 for RCC.
Results: The median uAQP-1 concentration among the cases and controls was 8.78 ng/mg creatinine (interquartile range [IQR]: 5.56–12.67) and 9.52 ng/mg creatinine (IQR: 5.55–12.45), respectively. There was no significant difference in uAQP-1 concentrations between the two groups. ROC analysis showed that, for a cutoff value of 8 ng/mg creatinine, the sensitivity and specificity of uAQP-1 as a diagnostic test were 47.2% and 66.7%, respectively, and area under the curve was 0.52 (95% confidence interval: 0.42–0.62).
Conclusions: uAQP-1 concentrations did not discriminate between healthy individuals and patients with RCC. The results of this study suggest that uAQP-1 may not be a suitable diagnostic biomarker for RCC in the study population. |
url |
http://www.indianjurol.com/article.asp?issn=0970-1591;year=2021;volume=37;issue=1;spage=59;epage=64;aulast=Cheriyan |
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