Characterization of hepatitis B and delta coinfection in Israel
Abstract Background Characteristics of hepatitis B (HBV) and delta (HDV) coinfection in various geographical regions, including Israel, remain unclear. Here we studied HDV seroprevalence in Israel, assessed HDV/HBV viral loads, circulating genotypes and hepatitis delta antigen (HDAg) conservation. M...
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2018-02-01
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doaj-6621bcbfdbcf42e7a5d94847a73a14f22020-11-25T03:39:22ZengBMCBMC Infectious Diseases1471-23342018-02-011811810.1186/s12879-018-3008-xCharacterization of hepatitis B and delta coinfection in IsraelRachel Shirazi0Daniela Ram1Aviya Rakovsky2Efrat Bucris3Yael Gozlan4Yaniv Lustig5Pninit Shaked-Mishan6Orit Picard7Yonat Shemer-Avni8Haim Ben-Zvi9Ora Halutz10Yoav Lurie11Ella Veizman12Matthias Carlebach13Marius Braun14Michal Cohen- Naftaly15Amir Shlomai16Rifaat Safadi17Ella Mendelson18Ella H. Sklan19Ziv Ben-Ari20Orna Mor21Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical CenterCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical CenterCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical CenterCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical CenterCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical CenterCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical CenterMicrobiology Laboratory, Carmel Medical CenterGastroenterology Laboratory, Sheba Medical CenterLaboratory of Clinical Virology, Soroka University Medical CenterMicrobiology Laboratory, Rabin Medical CenterMicrobiology Laboratory, Sorasky Medical CenterLiver Unit, Shaare Zedek Medical CenterLiver Unit, Rambam Medical CenterLiver Unit, Bnai Zion Medical CenterLiver Institute, Rabin Medical CenterLiver Institute, Rabin Medical CenterLiver Institute, Rabin Medical CenterLiver Unit, Hadassah Hebrew University HospitalCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical CenterThe Sackler School of Medicine, Tel Aviv UniversityThe Sackler School of Medicine, Tel Aviv UniversityCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical CenterAbstract Background Characteristics of hepatitis B (HBV) and delta (HDV) coinfection in various geographical regions, including Israel, remain unclear. Here we studied HDV seroprevalence in Israel, assessed HDV/HBV viral loads, circulating genotypes and hepatitis delta antigen (HDAg) conservation. Methods Serological anti HDV IgG results from 8969 HBsAg positive individuals tested in 2010-2015 were retrospectively analyzed to determine HDV seroprevalence. In a cohort of HBV/HDV coinfected (n=58) and HBV monoinfected (n=27) patients, quantitative real-time PCR (qRT-PCR) and sequencing were performed to determine viral loads, genotypes and hepatitis delta antigen (HDAg) protein sequence. Results 6.5% (587/8969) of the HBsAg positive patients were positive for anti HDV antibodies. HDV viral load was >2 log copies/ml higher than HBV viral load in most of the coinfected patients with detectable HDV RNA (86%, 50/58). HDV genotype 1 was identified in all patients, most of whom did not express HBV. While 66.6% (4/6) of the HBV/HDV co-expressing patients carried HBV-D2 only 18.5% (5/27) of the HBV monoinfections had HBV-D2 (p=0.03). Higher genetic variability in the HDAg protein sequence was associated with higher HDV viral load. Conclusions The overall significant prevalence of HDV (6.5%) mandates HDV RNA testing for all coinfected patients. Patients positive for HDV RNA (characterized by low HBV DNA blood levels) carried HDV genotype 1. Taken together, the significant HDV seroprevalence and the lack of effective anti-HDV therapy, necessitates strict clinical surveillance especially in patients with higher HDV viral loads and increased viral evolution.http://link.springer.com/article/10.1186/s12879-018-3008-xHepatitis delta (HDV)HDV genotypeSeroprevalenceHepatitis B (HBV)HBV genotypeHBV/HDV viral load |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rachel Shirazi Daniela Ram Aviya Rakovsky Efrat Bucris Yael Gozlan Yaniv Lustig Pninit Shaked-Mishan Orit Picard Yonat Shemer-Avni Haim Ben-Zvi Ora Halutz Yoav Lurie Ella Veizman Matthias Carlebach Marius Braun Michal Cohen- Naftaly Amir Shlomai Rifaat Safadi Ella Mendelson Ella H. Sklan Ziv Ben-Ari Orna Mor |
spellingShingle |
Rachel Shirazi Daniela Ram Aviya Rakovsky Efrat Bucris Yael Gozlan Yaniv Lustig Pninit Shaked-Mishan Orit Picard Yonat Shemer-Avni Haim Ben-Zvi Ora Halutz Yoav Lurie Ella Veizman Matthias Carlebach Marius Braun Michal Cohen- Naftaly Amir Shlomai Rifaat Safadi Ella Mendelson Ella H. Sklan Ziv Ben-Ari Orna Mor Characterization of hepatitis B and delta coinfection in Israel BMC Infectious Diseases Hepatitis delta (HDV) HDV genotype Seroprevalence Hepatitis B (HBV) HBV genotype HBV/HDV viral load |
author_facet |
Rachel Shirazi Daniela Ram Aviya Rakovsky Efrat Bucris Yael Gozlan Yaniv Lustig Pninit Shaked-Mishan Orit Picard Yonat Shemer-Avni Haim Ben-Zvi Ora Halutz Yoav Lurie Ella Veizman Matthias Carlebach Marius Braun Michal Cohen- Naftaly Amir Shlomai Rifaat Safadi Ella Mendelson Ella H. Sklan Ziv Ben-Ari Orna Mor |
author_sort |
Rachel Shirazi |
title |
Characterization of hepatitis B and delta coinfection in Israel |
title_short |
Characterization of hepatitis B and delta coinfection in Israel |
title_full |
Characterization of hepatitis B and delta coinfection in Israel |
title_fullStr |
Characterization of hepatitis B and delta coinfection in Israel |
title_full_unstemmed |
Characterization of hepatitis B and delta coinfection in Israel |
title_sort |
characterization of hepatitis b and delta coinfection in israel |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2018-02-01 |
description |
Abstract Background Characteristics of hepatitis B (HBV) and delta (HDV) coinfection in various geographical regions, including Israel, remain unclear. Here we studied HDV seroprevalence in Israel, assessed HDV/HBV viral loads, circulating genotypes and hepatitis delta antigen (HDAg) conservation. Methods Serological anti HDV IgG results from 8969 HBsAg positive individuals tested in 2010-2015 were retrospectively analyzed to determine HDV seroprevalence. In a cohort of HBV/HDV coinfected (n=58) and HBV monoinfected (n=27) patients, quantitative real-time PCR (qRT-PCR) and sequencing were performed to determine viral loads, genotypes and hepatitis delta antigen (HDAg) protein sequence. Results 6.5% (587/8969) of the HBsAg positive patients were positive for anti HDV antibodies. HDV viral load was >2 log copies/ml higher than HBV viral load in most of the coinfected patients with detectable HDV RNA (86%, 50/58). HDV genotype 1 was identified in all patients, most of whom did not express HBV. While 66.6% (4/6) of the HBV/HDV co-expressing patients carried HBV-D2 only 18.5% (5/27) of the HBV monoinfections had HBV-D2 (p=0.03). Higher genetic variability in the HDAg protein sequence was associated with higher HDV viral load. Conclusions The overall significant prevalence of HDV (6.5%) mandates HDV RNA testing for all coinfected patients. Patients positive for HDV RNA (characterized by low HBV DNA blood levels) carried HDV genotype 1. Taken together, the significant HDV seroprevalence and the lack of effective anti-HDV therapy, necessitates strict clinical surveillance especially in patients with higher HDV viral loads and increased viral evolution. |
topic |
Hepatitis delta (HDV) HDV genotype Seroprevalence Hepatitis B (HBV) HBV genotype HBV/HDV viral load |
url |
http://link.springer.com/article/10.1186/s12879-018-3008-x |
work_keys_str_mv |
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