Reprogramming the antigen specificity of B cells using genome-editing technologies

Reprogramming the antigen specificity of B cells using genome-editing technologies

We have developed a method to introduce novel paratopes into the human antibody repertoire by modifying the immunoglobulin (Ig) genes of mature B cells directly using genome editing technologies. We used CRISPR-Cas9 in a homology directed repair strategy, to replace the heavy chain (HC) variable reg...

Full description

Bibliographic Details
Main Authors: James E Voss, Alicia Gonzalez-Martin, Raiees Andrabi, Roberta P Fuller, Ben Murrell, Laura E McCoy, Katelyn Porter, Deli Huang, Wenjuan Li, Devin Sok, Khoa Le, Bryan Briney, Morgan Chateau, Geoffrey Rogers, Lars Hangartner, Ann J Feeney, David Nemazee, Paula Cannon, Dennis R Burton
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-01-01
Series:eLife
Subjects:
CAR-B
B cell
engineering
HIV
bnAb
vaccine
Online Access:https://elifesciences.org/articles/42995

Internet

https://elifesciences.org/articles/42995

Similar Items