BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions
Atrophic non-unions are a major clinical problem. Mineral coated microparticles (MCM) are electrolyte-coated hydroxyapatite particles that have been shown in vitro to bind growth factors electrostatically and enable a tuneable sustained release. Herein, we studied whether MCM can be used in vivo to...
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AO Research Institute Davos
2017-01-01
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doaj-68a4b6bc737b4851ab375c2a94423c572020-11-24T23:19:48Zeng AO Research Institute DavosEuropean Cells & Materials1473-22622017-01-013311210.22203/eCM.v033a01BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unionsM OrthNJ KruseBJ BraunC ScheuerJH HolsteinA Khalil X YuWL MurphyT Pohlemann MW LaschkeMD MengerAtrophic non-unions are a major clinical problem. Mineral coated microparticles (MCM) are electrolyte-coated hydroxyapatite particles that have been shown in vitro to bind growth factors electrostatically and enable a tuneable sustained release. Herein, we studied whether MCM can be used in vivo to apply Bone Morphogenetic Protein-2 (BMP-2) to improve bone repair of atrophic non-unions. For this purpose, atrophic non-unions were induced in femurs of CD-1 mice (n = 48). Animals either received BMP-2-coated MCM (MCM + BMP; n = 16), uncoated MCM (MCM; n = 16) or no MCM (NONE; n = 16). Bone healing was evaluated 2 and 10 weeks postoperatively by micro-computed tomographic (µCT), biomechanical, histomorphometric and immunohistochemical analyses. µCT revealed more bone volume with more highly mineralised bone in MCM + BMP femurs. Femurs of MCM + BMP animals showed a significantly higher bending stiffness compared to other groups. Histomorphometry further demonstrated that the callus of MCM + BMP femurs was larger and contained more bone and less fibrous tissue. After 10 weeks, 7 of 8 MCM + BMP femurs presented with complete osseous bridging, whereas NONE femurs exhibited a non-union rate of 100 %. Of interest, immunohistochemistry could not detect macrophages within the callus, indicating a good biocompatibility of MCM. In conclusion, the local application of BMP-2-coated MCM improved bone healing in a challenging murine non-union model and, thus, should be of clinical interest in the treatment of non-unions.http://www.ecmjournal.org/papers/vol033/pdf/v033a01.pdfNon-unionmineral coated microparticlesBMP-2bone healingbiocompatibilitymice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M Orth NJ Kruse BJ Braun C Scheuer JH Holstein A Khalil X Yu WL Murphy T Pohlemann MW Laschke MD Menger |
spellingShingle |
M Orth NJ Kruse BJ Braun C Scheuer JH Holstein A Khalil X Yu WL Murphy T Pohlemann MW Laschke MD Menger BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions European Cells & Materials Non-union mineral coated microparticles BMP-2 bone healing biocompatibility mice |
author_facet |
M Orth NJ Kruse BJ Braun C Scheuer JH Holstein A Khalil X Yu WL Murphy T Pohlemann MW Laschke MD Menger |
author_sort |
M Orth |
title |
BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions |
title_short |
BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions |
title_full |
BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions |
title_fullStr |
BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions |
title_full_unstemmed |
BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions |
title_sort |
bmp-2-coated mineral coated microparticles improve bone repair in atrophic non-unions |
publisher |
AO Research Institute Davos |
series |
European Cells & Materials |
issn |
1473-2262 |
publishDate |
2017-01-01 |
description |
Atrophic non-unions are a major clinical problem. Mineral coated microparticles (MCM) are electrolyte-coated hydroxyapatite particles that have been shown in vitro to bind growth factors electrostatically and enable a tuneable sustained release. Herein, we studied whether MCM can be used in vivo to apply Bone Morphogenetic Protein-2 (BMP-2) to improve bone repair of atrophic non-unions. For this purpose, atrophic non-unions were induced in femurs of CD-1 mice (n = 48). Animals either received BMP-2-coated MCM (MCM + BMP; n = 16), uncoated MCM (MCM; n = 16) or no MCM (NONE; n = 16). Bone healing was evaluated 2 and 10 weeks postoperatively by micro-computed tomographic (µCT), biomechanical, histomorphometric and immunohistochemical analyses. µCT revealed more bone volume with more highly mineralised bone in MCM + BMP femurs. Femurs of MCM + BMP animals showed a significantly higher bending stiffness compared to other groups. Histomorphometry further demonstrated that the callus of MCM + BMP femurs was larger and contained more bone and less fibrous tissue. After 10 weeks, 7 of 8 MCM + BMP femurs presented with complete osseous bridging, whereas NONE femurs exhibited a non-union rate of 100 %. Of interest, immunohistochemistry could not detect macrophages within the callus, indicating a good biocompatibility of MCM. In conclusion, the local application of BMP-2-coated MCM improved bone healing in a challenging murine non-union model and, thus, should be of clinical interest in the treatment of non-unions. |
topic |
Non-union mineral coated microparticles BMP-2 bone healing biocompatibility mice |
url |
http://www.ecmjournal.org/papers/vol033/pdf/v033a01.pdf |
work_keys_str_mv |
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