Molecular Epidemiology of Myroides odoratimimus in Nosocomial Catheter-Related Infection at a General Hospital in China

Shuang Yang, Qian Liu, Zhen Shen, Hua Wang, Lei He Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaCorrespondence: Lei He; Hua WangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine...

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Main Authors: Yang S, Liu Q, Shen Z, Wang H, He L
Format: Article
Language:English
Published: Dove Medical Press 2020-06-01
Series:Infection and Drug Resistance
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Online Access:https://www.dovepress.com/molecular-epidemiology-of-myroides-odoratimimus-in-nosocomial-catheter-peer-reviewed-article-IDR
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spelling doaj-68e0e9ff28954a7d88b538f8682bb2c42020-11-25T02:45:16ZengDove Medical PressInfection and Drug Resistance1178-69732020-06-01Volume 131981199354802Molecular Epidemiology of Myroides odoratimimus in Nosocomial Catheter-Related Infection at a General Hospital in ChinaYang SLiu QShen ZWang HHe LShuang Yang, Qian Liu, Zhen Shen, Hua Wang, Lei He Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaCorrespondence: Lei He; Hua WangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, No. 160 Pujian Road, Pudong New District, Shanghai, People’s Republic of ChinaEmail leihe@shsmu.edu.cn; renjijywh@shsmu.edu.cnPurpose: Catheter-related infection (CRI) is one of the most frequent causes of hospitalizations for immunocompromised patients. A major challenge is the increased prevalence of Myroides odoratimimus. The purpose of the present study was to evaluate the clinical features and molecular characteristics of M. odoratimimus collected from a general hospital in Shanghai, China.Patients and Methods: From July 2015 to August 2016, a total of 22 isolates of M. odoratimimus were collected from inpatients respectively from the biliary and pancreatic surgery (6/22) and the urology department (16/22). Clonal relatedness among the isolates was assessed using pulsed-field gel electrophoresis (PFGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Moreover, the antimicrobial susceptibility tests were carried out using the Clinical and Laboratory Standards Institute (CLSI) broth microdilution method. The presence of antibiotic resistance genes was screened using the polymerase chain reaction (PCR) assay. Additionally, protein structure prediction was analyzed using PSIPRED and RaptorX.Results: PFGE differentiated these isolates into six possibly related clones from two different departments obtained during a distinct period, indicating clonal dissemination in the two departments. We compared the dendrograms of M. odoratimimus isolates obtained by MALDI-TOF MS with those obtained by PFGE and found that the coincidence rate between them was only 68.2%. All the M. odoratimimus isolates were highly resistant to most available antibiotics, including carbapenems. Furthermore, chromosome-encoded β-lactamases MUS-1 was confirmed by PCR in 6 of 22 Myroides odoratimimus isolates. Herein, we also reported a novel variant of blaMUS- 1 in the remaining 16 isolates, which encodes MUS-3 protein at position 60 (Valine to Alanine), differing from the structure of MUS-1.Conclusion: The opportunistic and extensively antibiotic-resistant Myroides odoratimimus has a small range of epidemics in these two different departments. Clinicians should be aware that M. odoratimimus may induce a severe nosocomial outbreak of catheter-related infections, particularly in immunocompromised patients.Keywords: Flavobacterium, Myroides odoratimimus, MUS-1, nosocomial, outbreakhttps://www.dovepress.com/molecular-epidemiology-of-myroides-odoratimimus-in-nosocomial-catheter-peer-reviewed-article-IDRflavobacteriummyroides odoratimimusmus-1nosocomialoutbreak
collection DOAJ
language English
format Article
sources DOAJ
author Yang S
Liu Q
Shen Z
Wang H
He L
spellingShingle Yang S
Liu Q
Shen Z
Wang H
He L
Molecular Epidemiology of Myroides odoratimimus in Nosocomial Catheter-Related Infection at a General Hospital in China
Infection and Drug Resistance
flavobacterium
myroides odoratimimus
mus-1
nosocomial
outbreak
author_facet Yang S
Liu Q
Shen Z
Wang H
He L
author_sort Yang S
title Molecular Epidemiology of Myroides odoratimimus in Nosocomial Catheter-Related Infection at a General Hospital in China
title_short Molecular Epidemiology of Myroides odoratimimus in Nosocomial Catheter-Related Infection at a General Hospital in China
title_full Molecular Epidemiology of Myroides odoratimimus in Nosocomial Catheter-Related Infection at a General Hospital in China
title_fullStr Molecular Epidemiology of Myroides odoratimimus in Nosocomial Catheter-Related Infection at a General Hospital in China
title_full_unstemmed Molecular Epidemiology of Myroides odoratimimus in Nosocomial Catheter-Related Infection at a General Hospital in China
title_sort molecular epidemiology of myroides odoratimimus in nosocomial catheter-related infection at a general hospital in china
publisher Dove Medical Press
series Infection and Drug Resistance
issn 1178-6973
publishDate 2020-06-01
description Shuang Yang, Qian Liu, Zhen Shen, Hua Wang, Lei He Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaCorrespondence: Lei He; Hua WangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, No. 160 Pujian Road, Pudong New District, Shanghai, People’s Republic of ChinaEmail leihe@shsmu.edu.cn; renjijywh@shsmu.edu.cnPurpose: Catheter-related infection (CRI) is one of the most frequent causes of hospitalizations for immunocompromised patients. A major challenge is the increased prevalence of Myroides odoratimimus. The purpose of the present study was to evaluate the clinical features and molecular characteristics of M. odoratimimus collected from a general hospital in Shanghai, China.Patients and Methods: From July 2015 to August 2016, a total of 22 isolates of M. odoratimimus were collected from inpatients respectively from the biliary and pancreatic surgery (6/22) and the urology department (16/22). Clonal relatedness among the isolates was assessed using pulsed-field gel electrophoresis (PFGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Moreover, the antimicrobial susceptibility tests were carried out using the Clinical and Laboratory Standards Institute (CLSI) broth microdilution method. The presence of antibiotic resistance genes was screened using the polymerase chain reaction (PCR) assay. Additionally, protein structure prediction was analyzed using PSIPRED and RaptorX.Results: PFGE differentiated these isolates into six possibly related clones from two different departments obtained during a distinct period, indicating clonal dissemination in the two departments. We compared the dendrograms of M. odoratimimus isolates obtained by MALDI-TOF MS with those obtained by PFGE and found that the coincidence rate between them was only 68.2%. All the M. odoratimimus isolates were highly resistant to most available antibiotics, including carbapenems. Furthermore, chromosome-encoded β-lactamases MUS-1 was confirmed by PCR in 6 of 22 Myroides odoratimimus isolates. Herein, we also reported a novel variant of blaMUS- 1 in the remaining 16 isolates, which encodes MUS-3 protein at position 60 (Valine to Alanine), differing from the structure of MUS-1.Conclusion: The opportunistic and extensively antibiotic-resistant Myroides odoratimimus has a small range of epidemics in these two different departments. Clinicians should be aware that M. odoratimimus may induce a severe nosocomial outbreak of catheter-related infections, particularly in immunocompromised patients.Keywords: Flavobacterium, Myroides odoratimimus, MUS-1, nosocomial, outbreak
topic flavobacterium
myroides odoratimimus
mus-1
nosocomial
outbreak
url https://www.dovepress.com/molecular-epidemiology-of-myroides-odoratimimus-in-nosocomial-catheter-peer-reviewed-article-IDR
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