Metabolic and Redox Signaling of the Nucleoredoxin-Like-1 Gene for the Treatment of Genetic Retinal Diseases
The loss of cone photoreceptor function in retinitis pigmentosa (RP) severely impacts the central and daily vision and quality of life of patients affected by this disease. The loss of cones follows the degeneration of rods, in a manner independent of the causing mutations in numerous genes associat...
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doaj-6bb5a2de65dd43bd9b198907f43939b42020-11-25T00:42:31ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01215162510.3390/ijms21051625ijms21051625Metabolic and Redox Signaling of the Nucleoredoxin-Like-1 Gene for the Treatment of Genetic Retinal DiseasesEmmanuelle Clérin0Myriam Marussig1José-Alain Sahel2Thierry Léveillard3Department of Genetics, Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, FranceSparingVision, 55 rue de Lyon, 75012 Paris, FranceDepartment of Genetics, Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, FranceDepartment of Genetics, Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, FranceThe loss of cone photoreceptor function in retinitis pigmentosa (RP) severely impacts the central and daily vision and quality of life of patients affected by this disease. The loss of cones follows the degeneration of rods, in a manner independent of the causing mutations in numerous genes associated with RP. We have explored this phenomenon and proposed that the loss of rods triggers a reduction in the expression of rod-derived cone viability factor (RdCVF) encoded by the nucleoredoxin-like 1 (<i>NXNL1</i>) gene which interrupts the metabolic and redox signaling between rods and cones. After providing scientific evidence supporting this mechanism, we propose a way to restore this lost signaling and prevent the cone vision loss in animal models of RP. We also explain how we could restore this signaling to prevent cone vision loss in animal models of the disease and how we plan to apply this therapeutic strategy by the administration of both products of <i>NXNL1</i> encoding the trophic factor RdCVF and the thioredoxin enzyme RdCVFL using an adeno-associated viral vector. We describe in detail all the steps of this translational program, from the design of the drug, its production, biological validation, and analytical and preclinical qualification required for a future clinical trial that would, if successful, provide a treatment for this incurable disease.https://www.mdpi.com/1422-0067/21/5/1625retinitis pigmentosacone photoreceptorcentral visionrod-derived cone viability factoraerobic glycolysisthioredoxin signalinggene therapyadeno-associated viral vectorchemical manufacturingclinical trial |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emmanuelle Clérin Myriam Marussig José-Alain Sahel Thierry Léveillard |
spellingShingle |
Emmanuelle Clérin Myriam Marussig José-Alain Sahel Thierry Léveillard Metabolic and Redox Signaling of the Nucleoredoxin-Like-1 Gene for the Treatment of Genetic Retinal Diseases International Journal of Molecular Sciences retinitis pigmentosa cone photoreceptor central vision rod-derived cone viability factor aerobic glycolysis thioredoxin signaling gene therapy adeno-associated viral vector chemical manufacturing clinical trial |
author_facet |
Emmanuelle Clérin Myriam Marussig José-Alain Sahel Thierry Léveillard |
author_sort |
Emmanuelle Clérin |
title |
Metabolic and Redox Signaling of the Nucleoredoxin-Like-1 Gene for the Treatment of Genetic Retinal Diseases |
title_short |
Metabolic and Redox Signaling of the Nucleoredoxin-Like-1 Gene for the Treatment of Genetic Retinal Diseases |
title_full |
Metabolic and Redox Signaling of the Nucleoredoxin-Like-1 Gene for the Treatment of Genetic Retinal Diseases |
title_fullStr |
Metabolic and Redox Signaling of the Nucleoredoxin-Like-1 Gene for the Treatment of Genetic Retinal Diseases |
title_full_unstemmed |
Metabolic and Redox Signaling of the Nucleoredoxin-Like-1 Gene for the Treatment of Genetic Retinal Diseases |
title_sort |
metabolic and redox signaling of the nucleoredoxin-like-1 gene for the treatment of genetic retinal diseases |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-02-01 |
description |
The loss of cone photoreceptor function in retinitis pigmentosa (RP) severely impacts the central and daily vision and quality of life of patients affected by this disease. The loss of cones follows the degeneration of rods, in a manner independent of the causing mutations in numerous genes associated with RP. We have explored this phenomenon and proposed that the loss of rods triggers a reduction in the expression of rod-derived cone viability factor (RdCVF) encoded by the nucleoredoxin-like 1 (<i>NXNL1</i>) gene which interrupts the metabolic and redox signaling between rods and cones. After providing scientific evidence supporting this mechanism, we propose a way to restore this lost signaling and prevent the cone vision loss in animal models of RP. We also explain how we could restore this signaling to prevent cone vision loss in animal models of the disease and how we plan to apply this therapeutic strategy by the administration of both products of <i>NXNL1</i> encoding the trophic factor RdCVF and the thioredoxin enzyme RdCVFL using an adeno-associated viral vector. We describe in detail all the steps of this translational program, from the design of the drug, its production, biological validation, and analytical and preclinical qualification required for a future clinical trial that would, if successful, provide a treatment for this incurable disease. |
topic |
retinitis pigmentosa cone photoreceptor central vision rod-derived cone viability factor aerobic glycolysis thioredoxin signaling gene therapy adeno-associated viral vector chemical manufacturing clinical trial |
url |
https://www.mdpi.com/1422-0067/21/5/1625 |
work_keys_str_mv |
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