Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems
Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operation...
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doaj-6c7dbb2f52ae423aa7dd024aeedb3d1e2020-11-24T22:07:23ZengMDPI AGAntibodies2073-44682019-07-01834010.3390/antib8030040antib8030040Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase SystemsThomas Kruse0Axel Schmidt1Markus Kampmann2Jochen Strube3Institute for Separation and Process Technology, Clausthal University of Technology, Leibnizstraße 15, 38678 Clausthal-Zellerfeld, GermanyInstitute for Separation and Process Technology, Clausthal University of Technology, Leibnizstraße 15, 38678 Clausthal-Zellerfeld, GermanySartorius Stedim Biotech GmbH, August Spindler Straße 11, 37079 Göttingen, GermanyInstitute for Separation and Process Technology, Clausthal University of Technology, Leibnizstraße 15, 38678 Clausthal-Zellerfeld, GermanyTherapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operations in the downstream process. As an alternative approach to the conventional downstream process, a selective mAb extraction via an aqueous two-phase system (ATPS) directly from the cultivation broth of a mAb producing industrial relevant chinese hamster ovary (CHO) cell line was investigated. An efficient purification of the mAb was accomplished by the ATPS composition. The phase separation was realized by a newly developed membrane based phase separator. Moreover, a complete cell removal was integrated into this process by the used membrane. A selectivity between both phases was achieved by membrane modification. Yields up to 93% in the light phase and removal of process related impurities were obtained after aqueous two-phase extraction (ATPE). Phase separation performance as well as contact angles on the membrane were characterized for different ATPS. ATPE directly from the cultivation broth in combination with the new membrane based phase separation led to a mAb yield of 78% with a simultaneous reduction of deoxyribonucleic acid (DNA) and host cell protein (HCP) load.https://www.mdpi.com/2073-4468/8/3/40aqueous two-phase extractionphase separationdownstreamclarificationmembrane |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thomas Kruse Axel Schmidt Markus Kampmann Jochen Strube |
spellingShingle |
Thomas Kruse Axel Schmidt Markus Kampmann Jochen Strube Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems Antibodies aqueous two-phase extraction phase separation downstream clarification membrane |
author_facet |
Thomas Kruse Axel Schmidt Markus Kampmann Jochen Strube |
author_sort |
Thomas Kruse |
title |
Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems |
title_short |
Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems |
title_full |
Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems |
title_fullStr |
Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems |
title_full_unstemmed |
Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems |
title_sort |
integrated clarification and purification of monoclonal antibodies by membrane based separation of aqueous two-phase systems |
publisher |
MDPI AG |
series |
Antibodies |
issn |
2073-4468 |
publishDate |
2019-07-01 |
description |
Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operations in the downstream process. As an alternative approach to the conventional downstream process, a selective mAb extraction via an aqueous two-phase system (ATPS) directly from the cultivation broth of a mAb producing industrial relevant chinese hamster ovary (CHO) cell line was investigated. An efficient purification of the mAb was accomplished by the ATPS composition. The phase separation was realized by a newly developed membrane based phase separator. Moreover, a complete cell removal was integrated into this process by the used membrane. A selectivity between both phases was achieved by membrane modification. Yields up to 93% in the light phase and removal of process related impurities were obtained after aqueous two-phase extraction (ATPE). Phase separation performance as well as contact angles on the membrane were characterized for different ATPS. ATPE directly from the cultivation broth in combination with the new membrane based phase separation led to a mAb yield of 78% with a simultaneous reduction of deoxyribonucleic acid (DNA) and host cell protein (HCP) load. |
topic |
aqueous two-phase extraction phase separation downstream clarification membrane |
url |
https://www.mdpi.com/2073-4468/8/3/40 |
work_keys_str_mv |
AT thomaskruse integratedclarificationandpurificationofmonoclonalantibodiesbymembranebasedseparationofaqueoustwophasesystems AT axelschmidt integratedclarificationandpurificationofmonoclonalantibodiesbymembranebasedseparationofaqueoustwophasesystems AT markuskampmann integratedclarificationandpurificationofmonoclonalantibodiesbymembranebasedseparationofaqueoustwophasesystems AT jochenstrube integratedclarificationandpurificationofmonoclonalantibodiesbymembranebasedseparationofaqueoustwophasesystems |
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