Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar Rats
Epidemiological and clinical studies indicate a close relationship between obesity and asthma. Here, we determined the impact of diet-induced obesity on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA as well as IL-1β protein level and pathological changes in male Wistar rat’s lung after sen...
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Tehran University of Medical Sciences
2016-06-01
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doaj-6de896fa9fb646e68e08fea5d84e8c502020-11-25T04:11:46ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492016-06-01153616Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar RatsMohammad Reza Aslani0Rana Keyhanmanesh1Amir Mehdi Khamaneh2Mohammad Ali Ebrahimi Saadatlou3Mehran Mesgari Abbasi4Mohammad Reza Alipour5Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranSchool of advanced medical sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranTuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Epidemiological and clinical studies indicate a close relationship between obesity and asthma. Here, we determined the impact of diet-induced obesity on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA as well as IL-1β protein level and pathological changes in male Wistar rat’s lung after sensitization with ovalbumin (OVA). Twenty male Wistar rats divided into four groups, control with normal diet (C+ND), OVA-sensitized with normal diet (S+ND), control with high-fat diet (C+HFD), and OVA-sensitized with high-fat diet (S+HFD). All rats fed for 12 weeks with standard pellets or high-fat diet while sensitization and challenging with OVA or saline were done for groups in the last month. In the end of intervention, lung was isolated and tested for the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA with real time-PCR method, and pathological changes were determined. Diet-induced obesity groups showed increased weight, obesity indexes and lipid profiles The expression levels of IL-1β mRNA in OVA-sensitization groups (S+ND and S+HFD) showed a significant increase compared with other groups. Also in S+HFD group, expression level of IRAK-1 and TRAF-6 mRNA were markedly higher than other groups (p<0.001). The pathological changes were marked in sensitized groups compared to non-sensitized groups; with marked increase in obese sensitized rat. The results showed that high fat diet caused overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA as well as IL-1β protein in an experimental model of asthma. Our results suggest that obese-asthmatic conditions may lead to the local production and activation of pro-inflammatory agents. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/357AsthmaIL-1βIRAK-1LungObesityTRAF-6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammad Reza Aslani Rana Keyhanmanesh Amir Mehdi Khamaneh Mohammad Ali Ebrahimi Saadatlou Mehran Mesgari Abbasi Mohammad Reza Alipour |
spellingShingle |
Mohammad Reza Aslani Rana Keyhanmanesh Amir Mehdi Khamaneh Mohammad Ali Ebrahimi Saadatlou Mehran Mesgari Abbasi Mohammad Reza Alipour Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar Rats Iranian Journal of Allergy, Asthma and Immunology Asthma IL-1β IRAK-1 Lung Obesity TRAF-6 |
author_facet |
Mohammad Reza Aslani Rana Keyhanmanesh Amir Mehdi Khamaneh Mohammad Ali Ebrahimi Saadatlou Mehran Mesgari Abbasi Mohammad Reza Alipour |
author_sort |
Mohammad Reza Aslani |
title |
Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar Rats |
title_short |
Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar Rats |
title_full |
Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar Rats |
title_fullStr |
Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar Rats |
title_full_unstemmed |
Lung Altered Expression of IL-1β mRNA and Its Signaling Pathway Molecules in Obese-asthmatic Male Wistar Rats |
title_sort |
lung altered expression of il-1β mrna and its signaling pathway molecules in obese-asthmatic male wistar rats |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Allergy, Asthma and Immunology |
issn |
1735-1502 1735-5249 |
publishDate |
2016-06-01 |
description |
Epidemiological and clinical studies indicate a close relationship between obesity and asthma. Here, we determined the impact of diet-induced obesity on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA as well as IL-1β protein level and pathological changes in male Wistar rat’s lung after sensitization with ovalbumin (OVA). Twenty male Wistar rats divided into four groups, control with normal diet (C+ND), OVA-sensitized with normal diet (S+ND), control with high-fat diet (C+HFD), and OVA-sensitized with high-fat diet (S+HFD). All rats fed for 12 weeks with standard pellets or high-fat diet while sensitization and challenging with OVA or saline were done for groups in the last month. In the end of intervention, lung was isolated and tested for the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA with real time-PCR method, and pathological changes were determined. Diet-induced obesity groups showed increased weight, obesity indexes and lipid profiles The expression levels of IL-1β mRNA in OVA-sensitization groups (S+ND and S+HFD) showed a significant increase compared with other groups. Also in S+HFD group, expression level of IRAK-1 and TRAF-6 mRNA were markedly higher than other groups (p<0.001). The pathological changes were marked in sensitized groups compared to non-sensitized groups; with marked increase in obese sensitized rat. The results showed that high fat diet caused overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA as well as IL-1β protein in an experimental model of asthma. Our results suggest that obese-asthmatic conditions may lead to the local production and activation of pro-inflammatory agents.
|
topic |
Asthma IL-1β IRAK-1 Lung Obesity TRAF-6 |
url |
https://ijaai.tums.ac.ir/index.php/ijaai/article/view/357 |
work_keys_str_mv |
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