Proteomics study of human cord blood reticulocyte-derived exosomes
Abstract Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human...
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doaj-6e212bd6253c48669cdaf24f5c9de7822020-12-08T04:38:46ZengNature Publishing GroupScientific Reports2045-23222018-09-018111110.1038/s41598-018-32386-2Proteomics study of human cord blood reticulocyte-derived exosomesMíriam Díaz-Varela0Armando de Menezes-Neto1Daniel Perez-Zsolt2Ana Gámez-Valero3Joan Seguí-Barber4Nuria Izquierdo-Useros5Javier Martinez-Picado6Carmen Fernández-Becerra7Hernando A. del Portillo8ISGlobal, Hospital Clínic - Universitat de BarcelonaISGlobal, Hospital Clínic - Universitat de BarcelonaIrsiCaixa AIDS Research InstituteISGlobal, Hospital Clínic - Universitat de BarcelonaISGlobal, Hospital Clínic - Universitat de BarcelonaIrsiCaixa AIDS Research InstituteIrsiCaixa AIDS Research InstituteISGlobal, Hospital Clínic - Universitat de BarcelonaISGlobal, Hospital Clínic - Universitat de BarcelonaAbstract Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis.https://doi.org/10.1038/s41598-018-32386-2Human Reticulocyte-derivedExtracellular VesiclesOver-represention GO AnalysisTransferrin ReceptorExosome Biogenesis |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Míriam Díaz-Varela Armando de Menezes-Neto Daniel Perez-Zsolt Ana Gámez-Valero Joan Seguí-Barber Nuria Izquierdo-Useros Javier Martinez-Picado Carmen Fernández-Becerra Hernando A. del Portillo |
spellingShingle |
Míriam Díaz-Varela Armando de Menezes-Neto Daniel Perez-Zsolt Ana Gámez-Valero Joan Seguí-Barber Nuria Izquierdo-Useros Javier Martinez-Picado Carmen Fernández-Becerra Hernando A. del Portillo Proteomics study of human cord blood reticulocyte-derived exosomes Scientific Reports Human Reticulocyte-derived Extracellular Vesicles Over-represention GO Analysis Transferrin Receptor Exosome Biogenesis |
author_facet |
Míriam Díaz-Varela Armando de Menezes-Neto Daniel Perez-Zsolt Ana Gámez-Valero Joan Seguí-Barber Nuria Izquierdo-Useros Javier Martinez-Picado Carmen Fernández-Becerra Hernando A. del Portillo |
author_sort |
Míriam Díaz-Varela |
title |
Proteomics study of human cord blood reticulocyte-derived exosomes |
title_short |
Proteomics study of human cord blood reticulocyte-derived exosomes |
title_full |
Proteomics study of human cord blood reticulocyte-derived exosomes |
title_fullStr |
Proteomics study of human cord blood reticulocyte-derived exosomes |
title_full_unstemmed |
Proteomics study of human cord blood reticulocyte-derived exosomes |
title_sort |
proteomics study of human cord blood reticulocyte-derived exosomes |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-09-01 |
description |
Abstract Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis. |
topic |
Human Reticulocyte-derived Extracellular Vesicles Over-represention GO Analysis Transferrin Receptor Exosome Biogenesis |
url |
https://doi.org/10.1038/s41598-018-32386-2 |
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