Validation of endogenous reference genes in rat cerebral cortex for RT-qPCR analyses in developmental toxicity studies
Relative gene expression data obtained from quantitative RT-PCR (RT-qPCR) experiments are dependent on appropriate normalization to represent true values. It is common to use constitutively expressed endogenous reference genes (RGs) for normalization, but for this strategy to be valid the RGs must b...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
PeerJ Inc.
2019-06-01
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Series: | PeerJ |
Subjects: | |
Online Access: | https://peerj.com/articles/7181.pdf |