Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability
Microemulsion-based organogels (MBGs) were effectively employed for the immobilization of four commonly used lipases. During the asymmetric hydrolysis of ketoprofen vinyl ester at 30 °C for 24 h, lipase from Rhizomucor miehei and Mucor javanicus immobilized in microemulsion-based organogels (RML MBG...
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doaj-6fe3f9f08b03433097783cbb575282382020-11-24T21:18:17ZengElsevierBiotechnology Reports2215-017X2015-09-017C1810.1016/j.btre.2015.04.003Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusabilityWei-Wei Zhang0Jun-Qi Jia1Na Wang2Cheng-Li Hu3Sheng-Yong Yang4Xiao-Qi Yu5Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR ChinaKey Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR ChinaKey Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR ChinaKey Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR ChinaState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610064, PR ChinaKey Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR ChinaMicroemulsion-based organogels (MBGs) were effectively employed for the immobilization of four commonly used lipases. During the asymmetric hydrolysis of ketoprofen vinyl ester at 30 °C for 24 h, lipase from Rhizomucor miehei and Mucor javanicus immobilized in microemulsion-based organogels (RML MBGs and MJL MBGs) maintained good enantioselectivities (eep were 86.2% and 99.2%, respectively), and their activities increased 12.8-fold and 7.8-fold, respectively, compared with their free forms. They gave higher yields compared with other lipase MBGs and exhibited better enantioselectivity than commercial immobilized lipases. Immobilization considerably increased the tolerance to organic solvents and high temperature. Both MJL MBGs and RML MBGs showed excellent reusability during 30 cycles of repeated 24 h reactions at 30 °C (over 40 days). The system maintained yields of greater than 50%, while the ees values of RML MBGs and MJL MBGs remained nearly constant at 95% and 88%, respectively.http://www.sciencedirect.com/science/article/pii/S2215017X15000223Enzyme BiocatalysisLipaseMicroemulsion-based organogelsImmobilizationKinetic resolutionStability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei-Wei Zhang Jun-Qi Jia Na Wang Cheng-Li Hu Sheng-Yong Yang Xiao-Qi Yu |
spellingShingle |
Wei-Wei Zhang Jun-Qi Jia Na Wang Cheng-Li Hu Sheng-Yong Yang Xiao-Qi Yu Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability Biotechnology Reports Enzyme Biocatalysis Lipase Microemulsion-based organogels Immobilization Kinetic resolution Stability |
author_facet |
Wei-Wei Zhang Jun-Qi Jia Na Wang Cheng-Li Hu Sheng-Yong Yang Xiao-Qi Yu |
author_sort |
Wei-Wei Zhang |
title |
Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability |
title_short |
Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability |
title_full |
Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability |
title_fullStr |
Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability |
title_full_unstemmed |
Improved activity of lipase immobilized in microemulsion-based organogels for (R, S)-ketoprofen ester resolution: Long-term stability and reusability |
title_sort |
improved activity of lipase immobilized in microemulsion-based organogels for (r, s)-ketoprofen ester resolution: long-term stability and reusability |
publisher |
Elsevier |
series |
Biotechnology Reports |
issn |
2215-017X |
publishDate |
2015-09-01 |
description |
Microemulsion-based organogels (MBGs) were effectively employed for the immobilization of four commonly used lipases. During the asymmetric hydrolysis of ketoprofen vinyl ester at 30 °C for 24 h, lipase from Rhizomucor miehei and Mucor javanicus immobilized in microemulsion-based organogels (RML MBGs and MJL MBGs) maintained good enantioselectivities (eep were 86.2% and 99.2%, respectively), and their activities increased 12.8-fold and 7.8-fold, respectively, compared with their free forms. They gave higher yields compared with other lipase MBGs and exhibited better enantioselectivity than commercial immobilized lipases. Immobilization considerably increased the tolerance to organic solvents and high temperature. Both MJL MBGs and RML MBGs showed excellent reusability during 30 cycles of repeated 24 h reactions at 30 °C (over 40 days). The system maintained yields of greater than 50%, while the ees values of RML MBGs and MJL MBGs remained nearly constant at 95% and 88%, respectively. |
topic |
Enzyme Biocatalysis Lipase Microemulsion-based organogels Immobilization Kinetic resolution Stability |
url |
http://www.sciencedirect.com/science/article/pii/S2215017X15000223 |
work_keys_str_mv |
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