Label-Free Infrared Spectral Histology of Skin Tissue Part I: Impact of Lumican on Extracellular Matrix Integrity
Proteoglycans (PG) play an important role in maintaining the extracellular matrix (ECM) integrity. Lumican, a small leucine rich PG, is one such actor capable of regulating such properties. In this study, the integrity of the dermis of lumican-deleted Lum–/– vs. wild-type mice was investigated by co...
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doaj-705a3fe75921405c901d753f161d554a2020-11-25T03:02:48ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-05-01810.3389/fcell.2020.00320527194Label-Free Infrared Spectral Histology of Skin Tissue Part I: Impact of Lumican on Extracellular Matrix IntegrityLise Nannan0Lise Nannan1Valérie Untereiner2Isabelle Proult3Isabelle Proult4Camille Boulagnon-Rombi5Camille Boulagnon-Rombi6Camille Boulagnon-Rombi7Charlie Colin-Pierre8Charlie Colin-Pierre9Charlie Colin-Pierre10Ganesh D. Sockalingum11Stéphane Brézillon12Stéphane Brézillon13Université de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, Reims, FranceCNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, FranceUniversité de Reims Champagne-Ardenne, Cellular and Tissue Imaging Platform, Reims, FranceUniversité de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, Reims, FranceCNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, FranceUniversité de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, Reims, FranceCNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, FranceLaboratoire de Pathologie, Centre Hospitalier Universitaire de Reims, Reims, FranceUniversité de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, Reims, FranceCNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, FranceBASF Beauty Care Solutions France SAS, Pulnoy, FranceUniversité de Reims Champagne-Ardenne, BioSpecT-BioSpectroscopie Translationnelle, EA7506, UFR de Pharmacie, Reims, FranceUniversité de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, Reims, FranceCNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire, Reims, FranceProteoglycans (PG) play an important role in maintaining the extracellular matrix (ECM) integrity. Lumican, a small leucine rich PG, is one such actor capable of regulating such properties. In this study, the integrity of the dermis of lumican-deleted Lum–/– vs. wild-type mice was investigated by conventional histology and by infrared spectral histology (IRSH). Infrared spectroscopy is a non-invasive, rapid, label-free and sensitive technique that allows to probe molecular vibrations of biomolecules present in a tissue. Our IRSH results obtained on control (WT, n = 3) and Lum–/– (n = 3) mice showed that different histological structures were identified by using K-means clustering and validated by hematoxylin eosin saffron (HES) staining. Furthermore, an important increase of the dermis thickness was observed in Lum–/– compared to WT mice. In terms of structural information, analysis of the spectral images also revealed an intra-group homogeneity and inter-group heterogeneity. In addition, type I collagen contribution was evaluated by HES and picrosirius red staining as well as with IRSH. Both techniques showed a strong remodeling of the ECM in Lum–/– mice due to the looseness of collagen fibers in the increased dermis space. These results confirmed the impact of lumican on the ECM integrity. The loss of collagen fibers organization due to the absence of lumican can potentially increase the accessibility of anti-cancer drugs to the tumor. These results are qualitatively interesting and would need further structural characterization of type I collagen fibers in terms of size, organization, and orientation.https://www.frontiersin.org/article/10.3389/fcell.2020.00320/fulllumicanextracellular matrixskin remodelinginfrared imaginghistology |
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English |
format |
Article |
sources |
DOAJ |
author |
Lise Nannan Lise Nannan Valérie Untereiner Isabelle Proult Isabelle Proult Camille Boulagnon-Rombi Camille Boulagnon-Rombi Camille Boulagnon-Rombi Charlie Colin-Pierre Charlie Colin-Pierre Charlie Colin-Pierre Ganesh D. Sockalingum Stéphane Brézillon Stéphane Brézillon |
spellingShingle |
Lise Nannan Lise Nannan Valérie Untereiner Isabelle Proult Isabelle Proult Camille Boulagnon-Rombi Camille Boulagnon-Rombi Camille Boulagnon-Rombi Charlie Colin-Pierre Charlie Colin-Pierre Charlie Colin-Pierre Ganesh D. Sockalingum Stéphane Brézillon Stéphane Brézillon Label-Free Infrared Spectral Histology of Skin Tissue Part I: Impact of Lumican on Extracellular Matrix Integrity Frontiers in Cell and Developmental Biology lumican extracellular matrix skin remodeling infrared imaging histology |
author_facet |
Lise Nannan Lise Nannan Valérie Untereiner Isabelle Proult Isabelle Proult Camille Boulagnon-Rombi Camille Boulagnon-Rombi Camille Boulagnon-Rombi Charlie Colin-Pierre Charlie Colin-Pierre Charlie Colin-Pierre Ganesh D. Sockalingum Stéphane Brézillon Stéphane Brézillon |
author_sort |
Lise Nannan |
title |
Label-Free Infrared Spectral Histology of Skin Tissue Part I: Impact of Lumican on Extracellular Matrix Integrity |
title_short |
Label-Free Infrared Spectral Histology of Skin Tissue Part I: Impact of Lumican on Extracellular Matrix Integrity |
title_full |
Label-Free Infrared Spectral Histology of Skin Tissue Part I: Impact of Lumican on Extracellular Matrix Integrity |
title_fullStr |
Label-Free Infrared Spectral Histology of Skin Tissue Part I: Impact of Lumican on Extracellular Matrix Integrity |
title_full_unstemmed |
Label-Free Infrared Spectral Histology of Skin Tissue Part I: Impact of Lumican on Extracellular Matrix Integrity |
title_sort |
label-free infrared spectral histology of skin tissue part i: impact of lumican on extracellular matrix integrity |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2020-05-01 |
description |
Proteoglycans (PG) play an important role in maintaining the extracellular matrix (ECM) integrity. Lumican, a small leucine rich PG, is one such actor capable of regulating such properties. In this study, the integrity of the dermis of lumican-deleted Lum–/– vs. wild-type mice was investigated by conventional histology and by infrared spectral histology (IRSH). Infrared spectroscopy is a non-invasive, rapid, label-free and sensitive technique that allows to probe molecular vibrations of biomolecules present in a tissue. Our IRSH results obtained on control (WT, n = 3) and Lum–/– (n = 3) mice showed that different histological structures were identified by using K-means clustering and validated by hematoxylin eosin saffron (HES) staining. Furthermore, an important increase of the dermis thickness was observed in Lum–/– compared to WT mice. In terms of structural information, analysis of the spectral images also revealed an intra-group homogeneity and inter-group heterogeneity. In addition, type I collagen contribution was evaluated by HES and picrosirius red staining as well as with IRSH. Both techniques showed a strong remodeling of the ECM in Lum–/– mice due to the looseness of collagen fibers in the increased dermis space. These results confirmed the impact of lumican on the ECM integrity. The loss of collagen fibers organization due to the absence of lumican can potentially increase the accessibility of anti-cancer drugs to the tumor. These results are qualitatively interesting and would need further structural characterization of type I collagen fibers in terms of size, organization, and orientation. |
topic |
lumican extracellular matrix skin remodeling infrared imaging histology |
url |
https://www.frontiersin.org/article/10.3389/fcell.2020.00320/full |
work_keys_str_mv |
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