SHED, PRF, and Chitosan as Three-Dimensional of Tissue Engineering for Dental Pulp Regeneration

• Main Authors: Kelvin Alfan Nola Anggrarista, Pamela Handy Cecilia, Ayu Anggraini Broto Nagoro, Tania Saskianti, Meircurius Dwi Condro Surboyo
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2021-01-01
• Series: Dental Hypotheses
• Subjects: chitosan; dental pulp; prf; shed; tissue engineering
• Online Access: http://www.dentalhypotheses.com/article.asp?issn=2155-8213;year=2021;volume=12;issue=1;spage=43;epage=46;aulast=Anggrarista
• ISSN: 2155-8213

The gold standard for pulpitis irreversible treatment is root canal treatment. However, it caused the loss of tooth vitality. To restore tooth vitality, materials that have regenerative ability in the pulp is needed. The exfoliated deciduous teeth stem cells (SHED) not only expressed specific markers for mesenchymal stem cells (MSC) but also induced the odontoblastic differentiation, and stimulating the formation of endothelium and fibroblast. The combination SHED with platelet rich fibrin (PRF) and chitosan were able to facilitate and increase the migration, proliferation and odontoblastic differentiation of dental pulp cells. Based on that fact, the combination of SHED, PRF and chitosan as three-dimensional tissue engineering is promising as new modality to pulp regeneration in the clinical setting. The purpose of this review is describing the potential combination of SHED, PRF, and chitosan scaffold as three-dimensional tissue engineering for pulp regeneration.