Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus
Propolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis sampl...
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2013-01-01
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doaj-72135f2fec1844a291c9884580f995192020-11-24T21:06:15ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-44052013-01-0144257758510.1590/S1517-83822013000200038S1517-83822013000200038Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinusLeticia Barrientos0Christian L. Herrera1Gloria Montenegro2Ximena Ortega3Jorge Veloz4Marysol Alvear5Alejandro Cuevas6Nicolás Saavedra7Luis A. Salazar8Universidad de La FronteraUniversidad de La FronteraPontificia Universidad Católica de ChilePontificia Universidad Católica de ChileUniversidad de La FronteraUniversidad de La FronteraUniversidad de La FronteraUniversidad de La FronteraUniversidad de La FronteraPropolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The minimum inhibitory concentration (MIC) was determined on S. mutans and S. sobrinus. All propolis samples were dominated by structures from native plant species. The characterization by HPLC/MS, evidenced the presence of quercetin, myricetin, kaempferol, rutine, pinocembrin, coumaric acid, caffeic acid and caffeic acid phenethyl ester, that have already been described in these propolis with conventional HPLC. Although all propolis samples inhibited the mutans streptococci growth, it was observed a wide spectrum of action (MIC 0.90 to 8.22 µgmL-1). Given that results it becomes increasingly evident the need of standardization procedures, where we combine both the determination of botanical and the chemical characterization of the extracts. Research conducted to date, describes a promising effectiveness of propolis in the prevention of caries and other diseases of the oral cavity, making it necessary to develop studies to identify and understand the therapeutic targets or mechanisms of molecular action of the various compounds present on them.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200038&lng=en&tlng=enpropolisbotanical characterizationchemical characterizationStreptococcus mutansStreptococcus sobrinus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leticia Barrientos Christian L. Herrera Gloria Montenegro Ximena Ortega Jorge Veloz Marysol Alvear Alejandro Cuevas Nicolás Saavedra Luis A. Salazar |
spellingShingle |
Leticia Barrientos Christian L. Herrera Gloria Montenegro Ximena Ortega Jorge Veloz Marysol Alvear Alejandro Cuevas Nicolás Saavedra Luis A. Salazar Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus Brazilian Journal of Microbiology propolis botanical characterization chemical characterization Streptococcus mutans Streptococcus sobrinus |
author_facet |
Leticia Barrientos Christian L. Herrera Gloria Montenegro Ximena Ortega Jorge Veloz Marysol Alvear Alejandro Cuevas Nicolás Saavedra Luis A. Salazar |
author_sort |
Leticia Barrientos |
title |
Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus |
title_short |
Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus |
title_full |
Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus |
title_fullStr |
Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus |
title_full_unstemmed |
Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus |
title_sort |
chemical and botanical characterization of chilean propolis and biological activity on cariogenic bacteria streptococcus mutans and streptococcus sobrinus |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
publishDate |
2013-01-01 |
description |
Propolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The minimum inhibitory concentration (MIC) was determined on S. mutans and S. sobrinus. All propolis samples were dominated by structures from native plant species. The characterization by HPLC/MS, evidenced the presence of quercetin, myricetin, kaempferol, rutine, pinocembrin, coumaric acid, caffeic acid and caffeic acid phenethyl ester, that have already been described in these propolis with conventional HPLC. Although all propolis samples inhibited the mutans streptococci growth, it was observed a wide spectrum of action (MIC 0.90 to 8.22 µgmL-1). Given that results it becomes increasingly evident the need of standardization procedures, where we combine both the determination of botanical and the chemical characterization of the extracts. Research conducted to date, describes a promising effectiveness of propolis in the prevention of caries and other diseases of the oral cavity, making it necessary to develop studies to identify and understand the therapeutic targets or mechanisms of molecular action of the various compounds present on them. |
topic |
propolis botanical characterization chemical characterization Streptococcus mutans Streptococcus sobrinus |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200038&lng=en&tlng=en |
work_keys_str_mv |
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