High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning
Summary: Two-photon functional imaging using genetically encoded calcium indicators (GECIs) is one prominent tool to map neural activity. Under optimized experimental conditions, GECIs detect single action potentials in individual cells with high accuracy. However, using current approaches, these op...
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doaj-72319e7b2cd04b86a9c68caebd23c5532020-11-25T03:09:21ZengElsevierCell Reports2211-12472020-02-0130825672580.e6High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line ScanningMarco Brondi0Monica Moroni1Dania Vecchia2Manuel Molano-Mazón3Stefano Panzeri4Tommaso Fellin5Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, Genova, Italy; Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, ItalyNeural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, Italy; Neural Computation Laboratory, Center for Neuroscience and Cognitive Systems @UniTn, Istituto Italiano di Tecnologia, Rovereto, Italy; Center for Mind and Brain Sciences (CIMeC), University of Trento, Trento, ItalyOptical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, Genova, Italy; Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, ItalyNeural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, Italy; Neural Computation Laboratory, Center for Neuroscience and Cognitive Systems @UniTn, Istituto Italiano di Tecnologia, Rovereto, ItalyNeural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, Italy; Neural Computation Laboratory, Center for Neuroscience and Cognitive Systems @UniTn, Istituto Italiano di Tecnologia, Rovereto, ItalyOptical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, Genova, Italy; Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, Italy; Corresponding authorSummary: Two-photon functional imaging using genetically encoded calcium indicators (GECIs) is one prominent tool to map neural activity. Under optimized experimental conditions, GECIs detect single action potentials in individual cells with high accuracy. However, using current approaches, these optimized conditions are never met when imaging large ensembles of neurons. Here, we developed a method that substantially increases the signal-to-noise ratio (SNR) of population imaging of GECIs by using galvanometric mirrors and fast smart line scan (SLS) trajectories. We validated our approach in anesthetized and awake mice on deep and dense GCaMP6 staining in the mouse barrel cortex during spontaneous and sensory-evoked activity. Compared to raster population imaging, SLS led to increased SNR, higher probability of detecting calcium events, and more precise identification of functional neuronal ensembles. SLS provides a cheap and easily implementable tool for high-accuracy population imaging of neural GCaMP6 signals by using galvanometric-based two-photon microscopes. : Using galvanometric mirrors and fast smart line scan trajectories, Brondi et al. present a method to significantly increase the signal-to-noise ratio in population GCaMP6s imaging. The method is validated in anesthetized and awake mice, and it leads to more precise identification of functional neuronal ensembles. Keywords: two-photon imaging, GCaMP6, barrel cortex, neuronal ensembles, spatiotemporal neural responseshttp://www.sciencedirect.com/science/article/pii/S2211124720301492 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marco Brondi Monica Moroni Dania Vecchia Manuel Molano-Mazón Stefano Panzeri Tommaso Fellin |
spellingShingle |
Marco Brondi Monica Moroni Dania Vecchia Manuel Molano-Mazón Stefano Panzeri Tommaso Fellin High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning Cell Reports |
author_facet |
Marco Brondi Monica Moroni Dania Vecchia Manuel Molano-Mazón Stefano Panzeri Tommaso Fellin |
author_sort |
Marco Brondi |
title |
High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning |
title_short |
High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning |
title_full |
High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning |
title_fullStr |
High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning |
title_full_unstemmed |
High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning |
title_sort |
high-accuracy detection of neuronal ensemble activity in two-photon functional microscopy using smart line scanning |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2020-02-01 |
description |
Summary: Two-photon functional imaging using genetically encoded calcium indicators (GECIs) is one prominent tool to map neural activity. Under optimized experimental conditions, GECIs detect single action potentials in individual cells with high accuracy. However, using current approaches, these optimized conditions are never met when imaging large ensembles of neurons. Here, we developed a method that substantially increases the signal-to-noise ratio (SNR) of population imaging of GECIs by using galvanometric mirrors and fast smart line scan (SLS) trajectories. We validated our approach in anesthetized and awake mice on deep and dense GCaMP6 staining in the mouse barrel cortex during spontaneous and sensory-evoked activity. Compared to raster population imaging, SLS led to increased SNR, higher probability of detecting calcium events, and more precise identification of functional neuronal ensembles. SLS provides a cheap and easily implementable tool for high-accuracy population imaging of neural GCaMP6 signals by using galvanometric-based two-photon microscopes. : Using galvanometric mirrors and fast smart line scan trajectories, Brondi et al. present a method to significantly increase the signal-to-noise ratio in population GCaMP6s imaging. The method is validated in anesthetized and awake mice, and it leads to more precise identification of functional neuronal ensembles. Keywords: two-photon imaging, GCaMP6, barrel cortex, neuronal ensembles, spatiotemporal neural responses |
url |
http://www.sciencedirect.com/science/article/pii/S2211124720301492 |
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