Effects of Orexin B on Swine Granulosa and Endothelial Cells
In addition to the well-known central modulatory role of orexins, we recently demonstrated a peripheral involvement in swine granulosa cells for orexin A and in adipose tissue for orexin B (OXB). The aim of present research was to verify immunolocalization of OXB and its potential role in modulating...
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doaj-72521df819e048aa89091d8597a9a7212021-07-01T00:28:08ZengMDPI AGAnimals2076-26152021-06-01111812181210.3390/ani11061812Effects of Orexin B on Swine Granulosa and Endothelial CellsFrancesca Grasselli0Simona Bussolati1Stefano Grolli2Rosanna Di Lecce3Cecilia Dall’Aglio4Giuseppina Basini5Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, ItalyDipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, ItalyDipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, ItalyDipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, ItalyDipartimento di Medicina Veterinaria, Università degli Studi di Perugia, Via San Costanzo 4, 06126 Perugia, ItalyDipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, ItalyIn addition to the well-known central modulatory role of orexins, we recently demonstrated a peripheral involvement in swine granulosa cells for orexin A and in adipose tissue for orexin B (OXB). The aim of present research was to verify immunolocalization of OXB and its potential role in modulating the main features of swine granulosa cells. In particular, we explored the effects on granulosa cell proliferation (through the incorporation of bromodeoxyuridine), cell metabolic activity (as indirect evaluation by the assessment of ATP), steroidogenic activity (by immunoenzymatic examination) and redox status (evaluating the production of superoxide anion by means of the WST test, production of nitric oxide through the use of the Griess test and the non-enzymatic reducing power by FRAP test). Our data point out that OXB does not modify granulosa cell growth, steroidogenesis and superoxide anion generation. On the contrary, the peptide stimulates (<i>p</i> < 0.05) nitric oxide output and non-enzymatic reducing power. Since new vessel growth is crucial for ovarian follicle development, a further aim of this study was to explore the expression of prepro-orexin and the effects of OXB on swine aortic endothelial cells. We found that the peptide is ineffective in modulating cell growth, while it inhibits redox status parameters. In addition, we demonstrated a stimulatory effect on angiogenesis evaluated in fibrin gel angiogenesis assay. Taken together, OXB appears to be potentially involved in the modulation of redox status in granulosa and endothelial cells and we could argue an involvement of the peptide in the follicular angiogenic events.https://www.mdpi.com/2076-2615/11/6/1812ovarian follicleoxidative stressestradiol 17 βprogesteroneangiogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Francesca Grasselli Simona Bussolati Stefano Grolli Rosanna Di Lecce Cecilia Dall’Aglio Giuseppina Basini |
spellingShingle |
Francesca Grasselli Simona Bussolati Stefano Grolli Rosanna Di Lecce Cecilia Dall’Aglio Giuseppina Basini Effects of Orexin B on Swine Granulosa and Endothelial Cells Animals ovarian follicle oxidative stress estradiol 17 β progesterone angiogenesis |
author_facet |
Francesca Grasselli Simona Bussolati Stefano Grolli Rosanna Di Lecce Cecilia Dall’Aglio Giuseppina Basini |
author_sort |
Francesca Grasselli |
title |
Effects of Orexin B on Swine Granulosa and Endothelial Cells |
title_short |
Effects of Orexin B on Swine Granulosa and Endothelial Cells |
title_full |
Effects of Orexin B on Swine Granulosa and Endothelial Cells |
title_fullStr |
Effects of Orexin B on Swine Granulosa and Endothelial Cells |
title_full_unstemmed |
Effects of Orexin B on Swine Granulosa and Endothelial Cells |
title_sort |
effects of orexin b on swine granulosa and endothelial cells |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2021-06-01 |
description |
In addition to the well-known central modulatory role of orexins, we recently demonstrated a peripheral involvement in swine granulosa cells for orexin A and in adipose tissue for orexin B (OXB). The aim of present research was to verify immunolocalization of OXB and its potential role in modulating the main features of swine granulosa cells. In particular, we explored the effects on granulosa cell proliferation (through the incorporation of bromodeoxyuridine), cell metabolic activity (as indirect evaluation by the assessment of ATP), steroidogenic activity (by immunoenzymatic examination) and redox status (evaluating the production of superoxide anion by means of the WST test, production of nitric oxide through the use of the Griess test and the non-enzymatic reducing power by FRAP test). Our data point out that OXB does not modify granulosa cell growth, steroidogenesis and superoxide anion generation. On the contrary, the peptide stimulates (<i>p</i> < 0.05) nitric oxide output and non-enzymatic reducing power. Since new vessel growth is crucial for ovarian follicle development, a further aim of this study was to explore the expression of prepro-orexin and the effects of OXB on swine aortic endothelial cells. We found that the peptide is ineffective in modulating cell growth, while it inhibits redox status parameters. In addition, we demonstrated a stimulatory effect on angiogenesis evaluated in fibrin gel angiogenesis assay. Taken together, OXB appears to be potentially involved in the modulation of redox status in granulosa and endothelial cells and we could argue an involvement of the peptide in the follicular angiogenic events. |
topic |
ovarian follicle oxidative stress estradiol 17 β progesterone angiogenesis |
url |
https://www.mdpi.com/2076-2615/11/6/1812 |
work_keys_str_mv |
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