Preclinical safety assessment of Angelica acutiloba using a 13-week repeated dose oral toxicity study in rats
Abstract Angelica acutiloba (AA), a Japanese species of Danggui, has been used worldwide as a traditional herbal medicine with several bioactivities including anti-diabetic, anti-allergic, anti-inflammatory, anti-tumor, and anti-obesity. However, there is lack of toxicological data available to eval...
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doaj-73069e1151cc44f1b2f58b045d6d2a802020-11-25T03:08:48ZengBMCLaboratory Animal Research2233-76602017-12-0133322323010.5625/lar.2017.33.3.223Preclinical safety assessment of Angelica acutiloba using a 13-week repeated dose oral toxicity study in ratsJun-Won Yun0Euna Kwon1Seung-Hyun Kim2Ji-Ran You3Yun-Soon Kim4In Ae Park5Hyeon Hoe Kim6Jeong-Hwan Che7Byeong-Cheol Kang8Department of Biotechnology, The Catholic University of KoreaDepartment of Experimental Animal Research, Biomedical Research Institute, Seoul National University HospitalDepartment of Experimental Animal Research, Biomedical Research Institute, Seoul National University HospitalDepartment of Experimental Animal Research, Biomedical Research Institute, Seoul National University HospitalDepartment of Experimental Animal Research, Biomedical Research Institute, Seoul National University HospitalDepartment of Pathology, Seoul National University College of MedicineDepartment of Urology, Seoul National University College of MedicineBiomedical Center for Animal Resource and Development, Seoul National University College of MedicineDepartment of Experimental Animal Research, Biomedical Research Institute, Seoul National University HospitalAbstract Angelica acutiloba (AA), a Japanese species of Danggui, has been used worldwide as a traditional herbal medicine with several bioactivities including anti-diabetic, anti-allergic, anti-inflammatory, anti-tumor, and anti-obesity. However, there is lack of toxicological data available to evaluate potential long-term toxicity and the no-observed-adverse-effect level (NOAEL) of AA extract in accordance with the test guidelines published by the Organization for Economic Cooperation and Development. In the 14-day repeat-dose toxicity study, no adverse effects on mortality, body weight change, clinical signs, and organ weights was found following repeat oral administration to rats for 14 days (125, 250, 500, 1000, and 2000 mg/kg body weight), leading that 2000 mg/kg is the highest recommended dose of AA extract for the 13-week repeat-dose oral toxicity study. In the 13-week repeat-dose oral toxicity study, the AA extract was orally administered to groups of rats for 13 weeks (125, 250, 500, 1000, and 2000 mg/kg body weight) to compare between control and AA extract groups. The administration of AA extract did not produce mortality or remarkable clinical signs during this 13-week study. And, the data revealed that there were no significant differences in food/water consumption, body weight, hematological parameters, clinical chemistry parameters, gross macroscopic findings, organ weight and histopathology in comparison to the control group. On the basis of these results, the subchronic NOAEL of the AA extract was more than 2000 mg/kg/day when tested in rats. And, the AA extract is considered safe to use orally as a traditional herbal medicine.http://link.springer.com/article/10.5625/lar.2017.33.3.223Angelica acutilobatraditional medicinetoxicitysubchronic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun-Won Yun Euna Kwon Seung-Hyun Kim Ji-Ran You Yun-Soon Kim In Ae Park Hyeon Hoe Kim Jeong-Hwan Che Byeong-Cheol Kang |
spellingShingle |
Jun-Won Yun Euna Kwon Seung-Hyun Kim Ji-Ran You Yun-Soon Kim In Ae Park Hyeon Hoe Kim Jeong-Hwan Che Byeong-Cheol Kang Preclinical safety assessment of Angelica acutiloba using a 13-week repeated dose oral toxicity study in rats Laboratory Animal Research Angelica acutiloba traditional medicine toxicity subchronic |
author_facet |
Jun-Won Yun Euna Kwon Seung-Hyun Kim Ji-Ran You Yun-Soon Kim In Ae Park Hyeon Hoe Kim Jeong-Hwan Che Byeong-Cheol Kang |
author_sort |
Jun-Won Yun |
title |
Preclinical safety assessment of Angelica acutiloba using a 13-week repeated dose oral toxicity study in rats |
title_short |
Preclinical safety assessment of Angelica acutiloba using a 13-week repeated dose oral toxicity study in rats |
title_full |
Preclinical safety assessment of Angelica acutiloba using a 13-week repeated dose oral toxicity study in rats |
title_fullStr |
Preclinical safety assessment of Angelica acutiloba using a 13-week repeated dose oral toxicity study in rats |
title_full_unstemmed |
Preclinical safety assessment of Angelica acutiloba using a 13-week repeated dose oral toxicity study in rats |
title_sort |
preclinical safety assessment of angelica acutiloba using a 13-week repeated dose oral toxicity study in rats |
publisher |
BMC |
series |
Laboratory Animal Research |
issn |
2233-7660 |
publishDate |
2017-12-01 |
description |
Abstract Angelica acutiloba (AA), a Japanese species of Danggui, has been used worldwide as a traditional herbal medicine with several bioactivities including anti-diabetic, anti-allergic, anti-inflammatory, anti-tumor, and anti-obesity. However, there is lack of toxicological data available to evaluate potential long-term toxicity and the no-observed-adverse-effect level (NOAEL) of AA extract in accordance with the test guidelines published by the Organization for Economic Cooperation and Development. In the 14-day repeat-dose toxicity study, no adverse effects on mortality, body weight change, clinical signs, and organ weights was found following repeat oral administration to rats for 14 days (125, 250, 500, 1000, and 2000 mg/kg body weight), leading that 2000 mg/kg is the highest recommended dose of AA extract for the 13-week repeat-dose oral toxicity study. In the 13-week repeat-dose oral toxicity study, the AA extract was orally administered to groups of rats for 13 weeks (125, 250, 500, 1000, and 2000 mg/kg body weight) to compare between control and AA extract groups. The administration of AA extract did not produce mortality or remarkable clinical signs during this 13-week study. And, the data revealed that there were no significant differences in food/water consumption, body weight, hematological parameters, clinical chemistry parameters, gross macroscopic findings, organ weight and histopathology in comparison to the control group. On the basis of these results, the subchronic NOAEL of the AA extract was more than 2000 mg/kg/day when tested in rats. And, the AA extract is considered safe to use orally as a traditional herbal medicine. |
topic |
Angelica acutiloba traditional medicine toxicity subchronic |
url |
http://link.springer.com/article/10.5625/lar.2017.33.3.223 |
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