Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neurons

Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neurons

Chotosan (CTS), a traditional herbal formula called Kampo medicine, was shown to be effective in the treatment of vascular dementia in a clinical study, and exerted protective effects against transient cerebral ischemia-induced cognitive impairment in mice. In the present study, we investigated the...

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Main Authors: Sachie Sasaki-Hamada, Azusa Suzuki, Emi Sanai, Kinzo Matsumoto, Jun-Ichiro Oka
Format: Article
Language:English
Published: Elsevier 2017-11-01
Series:Journal of Pharmacological Sciences
Subjects:
Chotosan
Neuroprotective effects
GluN2B
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861317301780
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spelling doaj-7344c1f8f5c94e8ea9539eb14e7183fd2020-11-25T00:36:00ZengElsevierJournal of Pharmacological Sciences1347-86132017-11-01135313413710.1016/j.jphs.2017.10.009Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neuronsSachie Sasaki-Hamada0Azusa Suzuki1Emi Sanai2Kinzo Matsumoto3Jun-Ichiro Oka4Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, JapanLaboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, JapanLaboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, JapanDivision of Medicinal Pharmacology, Institute of Natural Medicine, University of Toyama, JapanLaboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, JapanChotosan (CTS), a traditional herbal formula called Kampo medicine, was shown to be effective in the treatment of vascular dementia in a clinical study, and exerted protective effects against transient cerebral ischemia-induced cognitive impairment in mice. In the present study, we investigated the neuroprotective effects of CTS using primary cultured rat cortical neurons. CTS (250–1000 μg/mL) inhibited neuronal death induced by 100 μM glutamate. This glutamate-induced neuronal death was blocked by a GluN2B-, but not GluN2A-containing NMDA receptor antagonist. Therefore, the neuroprotective effects of CTS were related to an inhibition of GluN2B-containing NMDA receptor-mediated responses.http://www.sciencedirect.com/science/article/pii/S1347861317301780ChotosanNeuroprotective effectsGluN2B
collection DOAJ
language English
format Article
sources DOAJ
author Sachie Sasaki-Hamada
Azusa Suzuki
Emi Sanai
Kinzo Matsumoto
Jun-Ichiro Oka
spellingShingle Sachie Sasaki-Hamada
Azusa Suzuki
Emi Sanai
Kinzo Matsumoto
Jun-Ichiro Oka
Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neurons
Journal of Pharmacological Sciences
Chotosan
Neuroprotective effects
GluN2B
author_facet Sachie Sasaki-Hamada
Azusa Suzuki
Emi Sanai
Kinzo Matsumoto
Jun-Ichiro Oka
author_sort Sachie Sasaki-Hamada
title Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neurons
title_short Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neurons
title_full Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neurons
title_fullStr Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neurons
title_full_unstemmed Neuroprotection by chotosan, a Kampo formula, against glutamate excitotoxicity involves the inhibition of GluN2B-, but not GluN2A-containing NMDA receptor-mediated responses in primary cultured cortical neurons
title_sort neuroprotection by chotosan, a kampo formula, against glutamate excitotoxicity involves the inhibition of glun2b-, but not glun2a-containing nmda receptor-mediated responses in primary cultured cortical neurons
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2017-11-01
description Chotosan (CTS), a traditional herbal formula called Kampo medicine, was shown to be effective in the treatment of vascular dementia in a clinical study, and exerted protective effects against transient cerebral ischemia-induced cognitive impairment in mice. In the present study, we investigated the neuroprotective effects of CTS using primary cultured rat cortical neurons. CTS (250–1000 μg/mL) inhibited neuronal death induced by 100 μM glutamate. This glutamate-induced neuronal death was blocked by a GluN2B-, but not GluN2A-containing NMDA receptor antagonist. Therefore, the neuroprotective effects of CTS were related to an inhibition of GluN2B-containing NMDA receptor-mediated responses.
topic Chotosan
Neuroprotective effects
GluN2B
url http://www.sciencedirect.com/science/article/pii/S1347861317301780
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