Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS
Diverse peanut varieties are widely cultivated in China. However, few studies have investigated the effects of germination on the phenolic profiles and antioxidant activities of specific Chinese peanut cultivars. Therefore, this study was conducted to evaluate the effects of germination on total phe...
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doaj-737e37139a524cedaa75f6cec18565e82020-11-25T00:02:55ZengMDPI AGAntioxidants2076-39212019-02-01824710.3390/antiox8020047antiox8020047Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MSQiong-Qiong Yang0Lin Cheng1Zhi-Yuan Long2Hua-Bin Li3Anil Gunaratne4Ren-You Gan5Harold Corke6Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Resources & Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangdong Engineering Technology Research Center of Nutrition Translation, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaFaculty of Agricultural Sciences, Sabaragamuwa University of Sri Lanka, Belihuloya P.O. Box 02, Sri LankaDepartment of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDiverse peanut varieties are widely cultivated in China. However, few studies have investigated the effects of germination on the phenolic profiles and antioxidant activities of specific Chinese peanut cultivars. Therefore, this study was conducted to evaluate the effects of germination on total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, and phenolic profiles of seven peanut cultivars in China. The TPC, TFC, and antioxidant activities were determined by spectrophotometry, while phenolic profiles were analyzed by using ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS). The results found that germination significantly increased TPC, TFC, and antioxidant activity. Antioxidant activity was found to be closely related to TPC in germinated peanut extracts, which indicates that phenolics are the main contributors of antioxidants in germinated peanuts. In addition, germination induced significant changes in polyphenolic profiles. In the analyzed samples, 36 phenolic compounds were identified in which most were flavonoids. Overall, these findings highlight that germinated peanuts can be a good natural source of natural antioxidants for human consumption and functional food development.https://www.mdpi.com/2076-3921/8/2/47groundnutgerminationpolyphenolsflavonoidsmass analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiong-Qiong Yang Lin Cheng Zhi-Yuan Long Hua-Bin Li Anil Gunaratne Ren-You Gan Harold Corke |
spellingShingle |
Qiong-Qiong Yang Lin Cheng Zhi-Yuan Long Hua-Bin Li Anil Gunaratne Ren-You Gan Harold Corke Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS Antioxidants groundnut germination polyphenols flavonoids mass analysis |
author_facet |
Qiong-Qiong Yang Lin Cheng Zhi-Yuan Long Hua-Bin Li Anil Gunaratne Ren-You Gan Harold Corke |
author_sort |
Qiong-Qiong Yang |
title |
Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS |
title_short |
Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS |
title_full |
Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS |
title_fullStr |
Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS |
title_full_unstemmed |
Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS |
title_sort |
comparison of the phenolic profiles of soaked and germinated peanut cultivars via uplc-qtof-ms |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2019-02-01 |
description |
Diverse peanut varieties are widely cultivated in China. However, few studies have investigated the effects of germination on the phenolic profiles and antioxidant activities of specific Chinese peanut cultivars. Therefore, this study was conducted to evaluate the effects of germination on total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, and phenolic profiles of seven peanut cultivars in China. The TPC, TFC, and antioxidant activities were determined by spectrophotometry, while phenolic profiles were analyzed by using ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS). The results found that germination significantly increased TPC, TFC, and antioxidant activity. Antioxidant activity was found to be closely related to TPC in germinated peanut extracts, which indicates that phenolics are the main contributors of antioxidants in germinated peanuts. In addition, germination induced significant changes in polyphenolic profiles. In the analyzed samples, 36 phenolic compounds were identified in which most were flavonoids. Overall, these findings highlight that germinated peanuts can be a good natural source of natural antioxidants for human consumption and functional food development. |
topic |
groundnut germination polyphenols flavonoids mass analysis |
url |
https://www.mdpi.com/2076-3921/8/2/47 |
work_keys_str_mv |
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