Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper
ABSTRACT Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization of Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 strains were identified as Xanthomonas euvesicatoria...
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doaj-748b31f8f5754c98b5d13b6551df7d082020-11-24T21:39:43ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-440549suppl 124625910.1016/j.bjm.2017.08.011S1517-83822018000500246Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepperTaca VanchevaMariya StoyanovaElena Tasheva-TerzievaNevena BogatzevskaPenka MonchevaABSTRACT Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization of Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 strains were identified as Xanthomonas euvesicatoria and 30 as Xanthomonas vesicatoria using species-specific primers and polymerase chain reaction followed by restriction fragment length polymorphism analysis. To assess the genetic diversity of the strains, two methods (Random Amplified Polymorphic DNA and Repetitive Element Palindromic-Polymerase Chain Reaction) were applied. Discriminatory index was calculated and analysis of molecular variance was carried out.Combined random amplified polymorphic DNA analysis of the X. euvesicatoria strains with primers CUGEA-4 and CUGEA-6 had greater discriminative power (0.60) than repetitive element palindromic-polymerase chain reaction with ERIC and BOX A1R primers, which makes this method applicable for strain diversity evaluation. Discrimination among the X. vesicatoria strains was achieved by the use of ERIC primers and only for the Bulgarian strains. The results demonstrated that X. euvesicatoria was more diverse than X. vesicatoria and heterogeneity was observed mainly in the Bulgarian populations. According to the analysis of molecular variance, genetic variations in X. euvesicatoria were observed among and within populations from different regions, while the differences between the two countries were minor. Following the principal coordinates analysis, a relation between the climatic conditions of the regions and a genetic distance of the populations may be suggested.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500246&lng=en&tlng=enBacterial spotRFLPRAPDREP-PCRHeterogeneity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Taca Vancheva Mariya Stoyanova Elena Tasheva-Terzieva Nevena Bogatzevska Penka Moncheva |
spellingShingle |
Taca Vancheva Mariya Stoyanova Elena Tasheva-Terzieva Nevena Bogatzevska Penka Moncheva Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper Brazilian Journal of Microbiology Bacterial spot RFLP RAPD REP-PCR Heterogeneity |
author_facet |
Taca Vancheva Mariya Stoyanova Elena Tasheva-Terzieva Nevena Bogatzevska Penka Moncheva |
author_sort |
Taca Vancheva |
title |
Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper |
title_short |
Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper |
title_full |
Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper |
title_fullStr |
Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper |
title_full_unstemmed |
Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper |
title_sort |
molecular methods for diversity assessment among xanthomonads of bulgarian and macedonian pepper |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
description |
ABSTRACT Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization of Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 strains were identified as Xanthomonas euvesicatoria and 30 as Xanthomonas vesicatoria using species-specific primers and polymerase chain reaction followed by restriction fragment length polymorphism analysis. To assess the genetic diversity of the strains, two methods (Random Amplified Polymorphic DNA and Repetitive Element Palindromic-Polymerase Chain Reaction) were applied. Discriminatory index was calculated and analysis of molecular variance was carried out.Combined random amplified polymorphic DNA analysis of the X. euvesicatoria strains with primers CUGEA-4 and CUGEA-6 had greater discriminative power (0.60) than repetitive element palindromic-polymerase chain reaction with ERIC and BOX A1R primers, which makes this method applicable for strain diversity evaluation. Discrimination among the X. vesicatoria strains was achieved by the use of ERIC primers and only for the Bulgarian strains. The results demonstrated that X. euvesicatoria was more diverse than X. vesicatoria and heterogeneity was observed mainly in the Bulgarian populations. According to the analysis of molecular variance, genetic variations in X. euvesicatoria were observed among and within populations from different regions, while the differences between the two countries were minor. Following the principal coordinates analysis, a relation between the climatic conditions of the regions and a genetic distance of the populations may be suggested. |
topic |
Bacterial spot RFLP RAPD REP-PCR Heterogeneity |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500246&lng=en&tlng=en |
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