Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice

Background and Objective: Brain is not able to produce new neurons by neurogenesis after maturity. Neurogenesis after the maturity was reported in Hippocampus and subventricular areas in the brain. Rosa canina L has various vitamins and other valuable compounds such as polyphenols, carotenoid, carbo...

Full description

Bibliographic Details
Main Authors: Nastaran Amintaheri, Maryam Tehranipour, Saeedeh Zafar Balanezhad
Format: Article
Language:fas
Published: Golestan University of Medical Sciences 2018-03-01
Series:مجله دانشگاه علوم پزشکی گرگان
Subjects:
Online Access:http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1028&slc_lang=en&sid=1
id doaj-755c33d39f2145a690005183a9b92963
record_format Article
spelling doaj-755c33d39f2145a690005183a9b929632020-11-24T21:11:54ZfasGolestan University of Medical Sciences مجله دانشگاه علوم پزشکی گرگان1562-47652008-40802018-03-012015863Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in miceNastaran Amintaheri0Maryam Tehranipour1Saeedeh Zafar Balanezhad2 MSc in Animal Biology, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Associate Professor, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. maryam_tehranipour@mshdiau.ac.ir Assistant Professor, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Background and Objective: Brain is not able to produce new neurons by neurogenesis after maturity. Neurogenesis after the maturity was reported in Hippocampus and subventricular areas in the brain. Rosa canina L has various vitamins and other valuable compounds such as polyphenols, carotenoid, carbohydrates and fatty acids. This study was conducted to evaluate the effect of the alcoholic extract of the fruit of Rosa canina L plant on neuronal density of the hippocampus in animal model. Methods: In this experimental study 24 adult male mice were randomly allocated into 4 groups including: control and three treatent groups. Animals in treatment groups 1, 2 and 3 were received the alcoholic extract with extract with a dose of 25, 50, 75 mg/kg/bw intraperitoneally (IP), for 21 day continuously with an invertal of 24 hours, respectively. Animals in control group were received normal saline injection. One month after the first injection, the animals were anesthetized and brain gently was out of the skull. After processing, seven-micron serial sections were stained with blue toluidine and erythrosine. Different regions of the hippocampus were photographed and neuronal density was evaluated by stereological methods and was compared with control group. Results: The mean neuronal density of CA1 area of hippocampus in control and the treated group with a dose of 25, 50, 75 mg/kg/bw was 55±2, 70±3, 65±3 and 61±2, respectively. Neuronal density significantly increased in treatment group with dosage of 25 mg/kg/bw in compared to control group (P<0.05). The mean neuronal density of CA2 and CA3 area of hippocampus in treated group with a dose of 25, 50, 75 mg/kg/bw was not significant in compared to controls. Conclusion: This study showed that the alcoholic extract of the fruit of Rosa canina L plant with dosage of 25 mg/kg/bw increase neurons of the mice hippocampus.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1028&slc_lang=en&sid=1HippocampusNeuronal densityRosa canina LAlcoholic extract
collection DOAJ
language fas
format Article
sources DOAJ
author Nastaran Amintaheri
Maryam Tehranipour
Saeedeh Zafar Balanezhad
spellingShingle Nastaran Amintaheri
Maryam Tehranipour
Saeedeh Zafar Balanezhad
Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice
مجله دانشگاه علوم پزشکی گرگان
Hippocampus
Neuronal density
Rosa canina L
Alcoholic extract
author_facet Nastaran Amintaheri
Maryam Tehranipour
Saeedeh Zafar Balanezhad
author_sort Nastaran Amintaheri
title Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice
title_short Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice
title_full Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice
title_fullStr Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice
title_full_unstemmed Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice
title_sort effect of alcoholic extracts of rosa canina l fruit on hippocampus neuronal density in mice
publisher Golestan University of Medical Sciences
series مجله دانشگاه علوم پزشکی گرگان
issn 1562-4765
2008-4080
publishDate 2018-03-01
description Background and Objective: Brain is not able to produce new neurons by neurogenesis after maturity. Neurogenesis after the maturity was reported in Hippocampus and subventricular areas in the brain. Rosa canina L has various vitamins and other valuable compounds such as polyphenols, carotenoid, carbohydrates and fatty acids. This study was conducted to evaluate the effect of the alcoholic extract of the fruit of Rosa canina L plant on neuronal density of the hippocampus in animal model. Methods: In this experimental study 24 adult male mice were randomly allocated into 4 groups including: control and three treatent groups. Animals in treatment groups 1, 2 and 3 were received the alcoholic extract with extract with a dose of 25, 50, 75 mg/kg/bw intraperitoneally (IP), for 21 day continuously with an invertal of 24 hours, respectively. Animals in control group were received normal saline injection. One month after the first injection, the animals were anesthetized and brain gently was out of the skull. After processing, seven-micron serial sections were stained with blue toluidine and erythrosine. Different regions of the hippocampus were photographed and neuronal density was evaluated by stereological methods and was compared with control group. Results: The mean neuronal density of CA1 area of hippocampus in control and the treated group with a dose of 25, 50, 75 mg/kg/bw was 55±2, 70±3, 65±3 and 61±2, respectively. Neuronal density significantly increased in treatment group with dosage of 25 mg/kg/bw in compared to control group (P<0.05). The mean neuronal density of CA2 and CA3 area of hippocampus in treated group with a dose of 25, 50, 75 mg/kg/bw was not significant in compared to controls. Conclusion: This study showed that the alcoholic extract of the fruit of Rosa canina L plant with dosage of 25 mg/kg/bw increase neurons of the mice hippocampus.
topic Hippocampus
Neuronal density
Rosa canina L
Alcoholic extract
url http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1028&slc_lang=en&sid=1
work_keys_str_mv AT nastaranamintaheri effectofalcoholicextractsofrosacaninalfruitonhippocampusneuronaldensityinmice
AT maryamtehranipour effectofalcoholicextractsofrosacaninalfruitonhippocampusneuronaldensityinmice
AT saeedehzafarbalanezhad effectofalcoholicextractsofrosacaninalfruitonhippocampusneuronaldensityinmice
_version_ 1716752245694922752