An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on t...

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Main Authors: Francesca Costantini, Roald M. Tiggelaar, Riccardo Salvio, Marco Nardecchia, Stefan Schlautmann, Cesare Manetti, Han J. G. E. Gardeniers, Giampiero de Cesare, Domenico Caputo, Augusto Nascetti
Format: Article
Language:English
Published: MDPI AG 2017-12-01
Series:Biosensors
Subjects:
Online Access:https://www.mdpi.com/2079-6374/7/4/58
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spelling doaj-75742ba94bf542fbbf0e43d7b3746c082020-11-24T21:53:03ZengMDPI AGBiosensors2079-63742017-12-01745810.3390/bios7040058bios7040058An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical AssayFrancesca Costantini0Roald M. Tiggelaar1Riccardo Salvio2Marco Nardecchia3Stefan Schlautmann4Cesare Manetti5Han J. G. E. Gardeniers6Giampiero de Cesare7Domenico Caputo8Augusto Nascetti9School of Aerospace Engineering, Sapienza University of Rome, via Salaria n. 851/881, 00138 Rome, ItalyMesoscale Chemical Systems, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The NetherlandsDepartment of Chemistry, Sapienza University of Rome, p.le Aldo Moro n.5, 00185 Rome, ItalySchool of Aerospace Engineering, Sapienza University of Rome, via Salaria n. 851/881, 00138 Rome, ItalyMesoscale Chemical Systems, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The NetherlandsDepartment of Environmental Biology, Sapienza University of Rome, p.le Aldo Moro n.5, 00185 Rome ItalyMesoscale Chemical Systems, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The NetherlandsDepartment of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Via Eudossiana, 18, 00184 Rome, ItalyDepartment of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Via Eudossiana, 18, 00184 Rome, ItalySchool of Aerospace Engineering, Sapienza University of Rome, via Salaria n. 851/881, 00138 Rome, ItalyA lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent.https://www.mdpi.com/2079-6374/7/4/58microfluidic channelsurface functionalizationamorphous silicon photosensorschemiluminescent based assaylab-on-chip
collection DOAJ
language English
format Article
sources DOAJ
author Francesca Costantini
Roald M. Tiggelaar
Riccardo Salvio
Marco Nardecchia
Stefan Schlautmann
Cesare Manetti
Han J. G. E. Gardeniers
Giampiero de Cesare
Domenico Caputo
Augusto Nascetti
spellingShingle Francesca Costantini
Roald M. Tiggelaar
Riccardo Salvio
Marco Nardecchia
Stefan Schlautmann
Cesare Manetti
Han J. G. E. Gardeniers
Giampiero de Cesare
Domenico Caputo
Augusto Nascetti
An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
Biosensors
microfluidic channel
surface functionalization
amorphous silicon photosensors
chemiluminescent based assay
lab-on-chip
author_facet Francesca Costantini
Roald M. Tiggelaar
Riccardo Salvio
Marco Nardecchia
Stefan Schlautmann
Cesare Manetti
Han J. G. E. Gardeniers
Giampiero de Cesare
Domenico Caputo
Augusto Nascetti
author_sort Francesca Costantini
title An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_short An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_full An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_fullStr An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_full_unstemmed An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_sort all-glass microfluidic network with integrated amorphous silicon photosensors for on-chip monitoring of enzymatic biochemical assay
publisher MDPI AG
series Biosensors
issn 2079-6374
publishDate 2017-12-01
description A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent.
topic microfluidic channel
surface functionalization
amorphous silicon photosensors
chemiluminescent based assay
lab-on-chip
url https://www.mdpi.com/2079-6374/7/4/58
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