Macro-, Micro- and Nano-Roughness of Carbon-Based Interface with the Living Cells: Towards a Versatile Bio-Sensing Platform
Integration of living cells with nonbiological surfaces (substrates) of sensors, scaffolds, and implants implies severe restrictions on the interface quality and properties, which broadly cover all elements of the interaction between the living and artificial systems (materials, surface modification...
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doaj-7574b09d24e64300ab5ad7fb6e955f762020-11-25T03:23:06ZengMDPI AGSensors1424-82202020-09-01205028502810.3390/s20185028Macro-, Micro- and Nano-Roughness of Carbon-Based Interface with the Living Cells: Towards a Versatile Bio-Sensing PlatformLena Golubewa0Hamza Rehman1Tatsiana Kulahava2Renata Karpicz3Marian Baah4Tommy Kaplas5Ali Shah6Sergei Malykhin7Alexander Obraztsov8Danielis Rutkauskas9Marija Jankunec10Ieva Matulaitienė11Algirdas Selskis12Andrei Denisov13Yuri Svirko14Polina Kuzhir15Center for Physical Sciences and Technology, Sauletekio Ave. 3, LT-10257 Vilnius, LithuaniaInstitute of Photonics, University of Eastern Finland, Yliopistokatu 2, FI-80100 Joensuu, FinlandInstitute for Nuclear Problems, Belarusian State University, Bobruiskaya 11, 220030 Minsk, BelarusCenter for Physical Sciences and Technology, Sauletekio Ave. 3, LT-10257 Vilnius, LithuaniaInstitute of Photonics, University of Eastern Finland, Yliopistokatu 2, FI-80100 Joensuu, FinlandInstitute of Photonics, University of Eastern Finland, Yliopistokatu 2, FI-80100 Joensuu, FinlandDepartment of Micro and Nanosciences, Aalto University, FI-00076 Espoo, P.O. Box 13500, FinlandInstitute of Photonics, University of Eastern Finland, Yliopistokatu 2, FI-80100 Joensuu, FinlandInstitute of Photonics, University of Eastern Finland, Yliopistokatu 2, FI-80100 Joensuu, FinlandCenter for Physical Sciences and Technology, Sauletekio Ave. 3, LT-10257 Vilnius, LithuaniaInstitute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, LithuaniaCenter for Physical Sciences and Technology, Sauletekio Ave. 3, LT-10257 Vilnius, LithuaniaCenter for Physical Sciences and Technology, Sauletekio Ave. 3, LT-10257 Vilnius, LithuaniaDepartment of Biophysics, Belarusian State University, Nezavisimosti Ave. 4, 220030 Minsk, BelarusInstitute of Photonics, University of Eastern Finland, Yliopistokatu 2, FI-80100 Joensuu, FinlandInstitute for Nuclear Problems, Belarusian State University, Bobruiskaya 11, 220030 Minsk, BelarusIntegration of living cells with nonbiological surfaces (substrates) of sensors, scaffolds, and implants implies severe restrictions on the interface quality and properties, which broadly cover all elements of the interaction between the living and artificial systems (materials, surface modifications, drug-eluting coatings, etc.). Substrate materials must support cellular viability, preserve sterility, and at the same time allow real-time analysis and control of cellular activity. We have compared new substrates based on graphene and pyrolytic carbon (PyC) for the cultivation of living cells. These are PyC films of nanometer thickness deposited on SiO<sub>2</sub> and black silicon and graphene nanowall films composed of graphene flakes oriented perpendicular to the Si substrate. The structure, morphology, and interface properties of these substrates are analyzed in terms of their biocompatibility. The PyC demonstrates interface biocompatibility, promising for controlling cell proliferation and directional intercellular contact formation while as-grown graphene walls possess high hydrophobicity and poor biocompatibility. By performing experiments with C6 glioma cells we discovered that PyC is a cell-friendly coating that can be used without poly-<span style="font-variant: small-caps;">l</span>-lysine or other biopolymers for controlling cell adhesion. Thus, the opportunity to easily control the physical/chemical properties and nanotopography makes the PyC films a perfect candidate for the development of biosensors and 3D bioscaffolds.https://www.mdpi.com/1424-8220/20/18/5028pyrolytic carbongraphenegraphene nanowallsblack siliconglioma cellsbiocompatibility |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lena Golubewa Hamza Rehman Tatsiana Kulahava Renata Karpicz Marian Baah Tommy Kaplas Ali Shah Sergei Malykhin Alexander Obraztsov Danielis Rutkauskas Marija Jankunec Ieva Matulaitienė Algirdas Selskis Andrei Denisov Yuri Svirko Polina Kuzhir |
spellingShingle |
Lena Golubewa Hamza Rehman Tatsiana Kulahava Renata Karpicz Marian Baah Tommy Kaplas Ali Shah Sergei Malykhin Alexander Obraztsov Danielis Rutkauskas Marija Jankunec Ieva Matulaitienė Algirdas Selskis Andrei Denisov Yuri Svirko Polina Kuzhir Macro-, Micro- and Nano-Roughness of Carbon-Based Interface with the Living Cells: Towards a Versatile Bio-Sensing Platform Sensors pyrolytic carbon graphene graphene nanowalls black silicon glioma cells biocompatibility |
author_facet |
Lena Golubewa Hamza Rehman Tatsiana Kulahava Renata Karpicz Marian Baah Tommy Kaplas Ali Shah Sergei Malykhin Alexander Obraztsov Danielis Rutkauskas Marija Jankunec Ieva Matulaitienė Algirdas Selskis Andrei Denisov Yuri Svirko Polina Kuzhir |
author_sort |
Lena Golubewa |
title |
Macro-, Micro- and Nano-Roughness of Carbon-Based Interface with the Living Cells: Towards a Versatile Bio-Sensing Platform |
title_short |
Macro-, Micro- and Nano-Roughness of Carbon-Based Interface with the Living Cells: Towards a Versatile Bio-Sensing Platform |
title_full |
Macro-, Micro- and Nano-Roughness of Carbon-Based Interface with the Living Cells: Towards a Versatile Bio-Sensing Platform |
title_fullStr |
Macro-, Micro- and Nano-Roughness of Carbon-Based Interface with the Living Cells: Towards a Versatile Bio-Sensing Platform |
title_full_unstemmed |
Macro-, Micro- and Nano-Roughness of Carbon-Based Interface with the Living Cells: Towards a Versatile Bio-Sensing Platform |
title_sort |
macro-, micro- and nano-roughness of carbon-based interface with the living cells: towards a versatile bio-sensing platform |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2020-09-01 |
description |
Integration of living cells with nonbiological surfaces (substrates) of sensors, scaffolds, and implants implies severe restrictions on the interface quality and properties, which broadly cover all elements of the interaction between the living and artificial systems (materials, surface modifications, drug-eluting coatings, etc.). Substrate materials must support cellular viability, preserve sterility, and at the same time allow real-time analysis and control of cellular activity. We have compared new substrates based on graphene and pyrolytic carbon (PyC) for the cultivation of living cells. These are PyC films of nanometer thickness deposited on SiO<sub>2</sub> and black silicon and graphene nanowall films composed of graphene flakes oriented perpendicular to the Si substrate. The structure, morphology, and interface properties of these substrates are analyzed in terms of their biocompatibility. The PyC demonstrates interface biocompatibility, promising for controlling cell proliferation and directional intercellular contact formation while as-grown graphene walls possess high hydrophobicity and poor biocompatibility. By performing experiments with C6 glioma cells we discovered that PyC is a cell-friendly coating that can be used without poly-<span style="font-variant: small-caps;">l</span>-lysine or other biopolymers for controlling cell adhesion. Thus, the opportunity to easily control the physical/chemical properties and nanotopography makes the PyC films a perfect candidate for the development of biosensors and 3D bioscaffolds. |
topic |
pyrolytic carbon graphene graphene nanowalls black silicon glioma cells biocompatibility |
url |
https://www.mdpi.com/1424-8220/20/18/5028 |
work_keys_str_mv |
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