Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells

• Main Authors: Da-Yeon Lee, Yoon-Seok Chun, Jong-Kyu Kim, Jeong-Ok Lee, Young-Joon Lee, Sae-Kwang Ku, Soon-Mi Shim
• Format: Article
• Language: English
• Published: MDPI AG 2021-03-01
• Series: Antioxidants
• Subjects: spray dry powder containing 40% curcumin (CM-SD); antioxidant; anti-inflammatory; oxidative stress; Nrf2; C2C12 myoblast cells
• Online Access: https://www.mdpi.com/2076-3921/10/3/476

The purpose of the current study was to investigate antioxidant and anti-inflammatory effects of spray dry powder containing 40% curcumin (CM-SD) in C2C12 myoblast cells. CM-SD increased DPPH radical scavenging activity in a dose-dependent manner, and up to 30 μg/mL of CM-SD did not express cytotoxicity in C2C12 cells. Exposure to hydrogen peroxide (H₂O₂) drastically decreased the viability of C2C12 cells, but pre-treatment of CM-SD significantly increased the cell viability (<i>p</i> < 0.01). CM-SD significantly transactivated the nuclear factor erythroid-2-related factor 2 (Nrf2)-dependent luciferase activity in a dose-dependent manner and enhanced the levels of heme oxygenase (HO)-1, glutamate cysteine ligase catalytic subunit (GCLC), and NAD(P)H-dependent quinone oxidoreductase (NQO)-1. CM-SD also significantly reduced reactive oxygen species (ROS) production and lipid peroxidation and restored glutathione (GSH) depletion in H₂O₂-treated C2C12 cells. Moreover, CM-SD significantly reduced lipopolysaccharides (LPS)-mediated interleukin (IL)-6 production in the conditioned medium. Results from the current study suggest that CM-SD could be a useful candidate against oxidative stress and inflammation-related muscle disorders.