From 2D to 3D: Promising Advances in Imaging Lung Structure

From 2D to 3D: Promising Advances in Imaging Lung Structure

The delicate structure of murine lungs poses many challenges for acquiring high-quality images that truly represent the living lung. Here, we describe several optimized procedures for obtaining and imaging murine lung tissue. Compared to traditional paraffin cross-section and optimal cutting tempera...

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Bibliographic Details
Main Authors: Timothy Klouda, David Condon, Yuan Hao, Wen Tian, Maria Lvova, Ananya Chakraborty, Mark R. Nicolls, Xiaobo Zhou, Benjamin A. Raby, Ke Yuan
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-07-01
Series:Frontiers in Medicine
Subjects:
lung structure
vibratome
precision cut lung slices
optical clearing
confocal
STED
Online Access:https://www.frontiersin.org/article/10.3389/fmed.2020.00343/full

Internet

https://www.frontiersin.org/article/10.3389/fmed.2020.00343/full

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