Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form

Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form

Abstract A new transaminase (VbTA) was identified from the genome of the halotolerant marine bacterium Virgibacillus 21D. Following heterologous expression in Escherichia coli, it was located entirely in the insoluble fraction. After a single mutation, identified via sequence homology analyses, the...

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Bibliographic Details
Main Authors: Benedetta Guidi, Matteo Planchestainer, Martina Letizia Contente, Tommaso Laurenzi, Ivano Eberini, Louise J. Gourlay, Diego Romano, Francesca Paradisi, Francesco Molinari
Format: Article
Language:English
Published: Nature Publishing Group 2018-11-01
Series:Scientific Reports
Subjects:
Virgibacillus
Mutation T215F
Hydration Free Energy
Deep Hypersaline Anoxic Basins
Acceptor Amino
Online Access:https://doi.org/10.1038/s41598-018-34434-3

Internet

https://doi.org/10.1038/s41598-018-34434-3

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