NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions.

Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of ampl...

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Bibliographic Details
Main Authors: Andrey Kechin, Viktoria Borobova, Ulyana Boyarskikh, Evgeniy Khrapov, Sergey Subbotin, Maxim Filipenko
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-12-01
Series:PLoS Computational Biology
Online Access:https://doi.org/10.1371/journal.pcbi.1008468