Inhibitory Potential Of Lactobacillus Plantarum on Escherichia Coli

• Main Authors: Laura MITREA, Lavinia Florina CĂLINOIU, Gabriela PRECUP, Maria BINDEA, Bogdan RUSU, Monica TRIF, Lorand Jozsef FERENCZI, Bianca Eugenia ŞTEFĂNESCU, Dan Cristian VODNAR
• Format: Article
• Language: English
• Published: AcademicPres 2017-11-01
• Series: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology
• Subjects: E. coli, inhibitory potential, L. plantarum, pathogenic strains, probiotics
• Online Access: http://journals.usamvcluj.ro/index.php/fst/article/view/12767
• ISSN: 2344-2344; 2344-5300

An alternative therapy to diarrhoeal episodes induced by E. coli is represented by probiotic strains (e.g Lactobacillus), which are able to maintain the gut micro-flora in optimal parameters. This work highlights the inhibitory potential of probiotics (L. plantarum ATCC 8014) over the enteropathogenic bacteria like E. coli ATCC 25922. For this study we co-incubated L. plantarum with E. coli in MRS broth at different concentrations (109, 108 CFU/mL – L. plantarum with 105, 104 CFU/mL – E. coli, 4 groups) for 24, 48 and 72h at 37oC. After co-incubation, E. coli colonies developed on Levine media were counted towards the control. E. coli colonies decreased after 48 and 72h of co-incubation with L. plantarum. Also, was noticed that L. plantarum (108 CFU/mL) completely inhibits E. coli (104 CFU/mL) after 72h of co-incubation. This work proves the inhibitory potential of probiotic strains (L. plantarum ATCC 8014) against enteropathogenic bacteria (E. coli ATCC 25922).