Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations Analysis
The large-conductance voltage- and Ca<inline-formula><math display="inline"><semantics><msup><mrow></mrow><mrow><mn>2</mn><mo>+</mo></mrow></msup></semantics></math></inline-formula>-activated &...
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doaj-7d13a0df01de49908e5f891233b264a32020-11-25T02:45:35ZengMDPI AGCells2073-44092020-10-0192305230510.3390/cells9102305Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations AnalysisAgata Wawrzkiewicz-Jałowiecka0Paulina Trybek1Przemysław Borys2Beata Dworakowska3Łukasz Machura4Piotr Bednarczyk5Department of Physical Chemistry and Technology of Polymers, Silesian University of Technology, 44-100 Gliwice, PolandFaculty of Science and Technology, University of Silesia in Katowice, 41-500 Chorzow, PolandDepartment of Physical Chemistry and Technology of Polymers, Silesian University of Technology, 44-100 Gliwice, PolandInstitute of Biology, Department of Physics and Biophysics, Warsaw University of Life Sciences—SGGW, 02-787 Warszawa, PolandInstitute of Physics, Faculty of Science and Technology, University of Silesia in Katowice, 41-500 Chorzow, PolandInstitute of Biology, Department of Physics and Biophysics, Warsaw University of Life Sciences—SGGW, 02-787 Warszawa, PolandThe large-conductance voltage- and Ca<inline-formula><math display="inline"><semantics><msup><mrow></mrow><mrow><mn>2</mn><mo>+</mo></mrow></msup></semantics></math></inline-formula>-activated <inline-formula><math display="inline"><semantics><msup><mi>K</mi><mo>+</mo></msup></semantics></math></inline-formula> channels (BK) are encoded in humans by the Kcnma1 gene. Nevertheless, BK channel isoforms in different locations can exhibit functional heterogeneity mainly due to the alternative splicing during the Kcnma1 gene transcription. Here, we would like to examine the existence of dynamic diversity of BK channels from the inner mitochondrial and cellular membrane from human glioblastoma (U-87 MG). Not only the standard characteristics of the spontaneous switching between the functional states of the channel is discussed, but we put a special emphasis on the presence and strength of correlations within the signal describing the single-channel activity. The considered short- and long-range memory effects are here analyzed as they can be interpreted in terms of the complexity of the switching mechanism between stable conformational states of the channel. We calculate the dependencies of mean dwell-times of (conducting/non-conducting) states on the duration of the previous state, Hurst exponents by the rescaled range R/S method and detrended fluctuation analysis (DFA), and use the multifractal extension of the DFA (MFDFA) for the series describing single-channel activity. The obtained results unraveled statistically significant diversity in gating machinery between the mitochondrial and cellular BK channels.https://www.mdpi.com/2073-4409/9/10/2305glioblastomaBK channels in plasma membranemitochondrial BK (mitoBK) channelspatch-clamphurst exponentdetrended fluctuation analysis (DFA) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Agata Wawrzkiewicz-Jałowiecka Paulina Trybek Przemysław Borys Beata Dworakowska Łukasz Machura Piotr Bednarczyk |
spellingShingle |
Agata Wawrzkiewicz-Jałowiecka Paulina Trybek Przemysław Borys Beata Dworakowska Łukasz Machura Piotr Bednarczyk Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations Analysis Cells glioblastoma BK channels in plasma membrane mitochondrial BK (mitoBK) channels patch-clamp hurst exponent detrended fluctuation analysis (DFA) |
author_facet |
Agata Wawrzkiewicz-Jałowiecka Paulina Trybek Przemysław Borys Beata Dworakowska Łukasz Machura Piotr Bednarczyk |
author_sort |
Agata Wawrzkiewicz-Jałowiecka |
title |
Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations Analysis |
title_short |
Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations Analysis |
title_full |
Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations Analysis |
title_fullStr |
Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations Analysis |
title_full_unstemmed |
Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations Analysis |
title_sort |
differences in gating dynamics of bk channels in cellular and mitochondrial membranes from human glioblastoma cells unraveled by short- and long-range correlations analysis |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2020-10-01 |
description |
The large-conductance voltage- and Ca<inline-formula><math display="inline"><semantics><msup><mrow></mrow><mrow><mn>2</mn><mo>+</mo></mrow></msup></semantics></math></inline-formula>-activated <inline-formula><math display="inline"><semantics><msup><mi>K</mi><mo>+</mo></msup></semantics></math></inline-formula> channels (BK) are encoded in humans by the Kcnma1 gene. Nevertheless, BK channel isoforms in different locations can exhibit functional heterogeneity mainly due to the alternative splicing during the Kcnma1 gene transcription. Here, we would like to examine the existence of dynamic diversity of BK channels from the inner mitochondrial and cellular membrane from human glioblastoma (U-87 MG). Not only the standard characteristics of the spontaneous switching between the functional states of the channel is discussed, but we put a special emphasis on the presence and strength of correlations within the signal describing the single-channel activity. The considered short- and long-range memory effects are here analyzed as they can be interpreted in terms of the complexity of the switching mechanism between stable conformational states of the channel. We calculate the dependencies of mean dwell-times of (conducting/non-conducting) states on the duration of the previous state, Hurst exponents by the rescaled range R/S method and detrended fluctuation analysis (DFA), and use the multifractal extension of the DFA (MFDFA) for the series describing single-channel activity. The obtained results unraveled statistically significant diversity in gating machinery between the mitochondrial and cellular BK channels. |
topic |
glioblastoma BK channels in plasma membrane mitochondrial BK (mitoBK) channels patch-clamp hurst exponent detrended fluctuation analysis (DFA) |
url |
https://www.mdpi.com/2073-4409/9/10/2305 |
work_keys_str_mv |
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