Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk
A highly sensitive aptasensor for aflatoxin M1 (AFM1) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method and exhibited broad and...
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doaj-7dbc581af5f749f3bb69a2852a9a01352020-11-24T20:48:00ZengMDPI AGToxins2072-66512017-10-0191031810.3390/toxins9100318toxins9100318Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in MilkHui Li0Daibin Yang1Peiwu Li2Qi Zhang3Wen Zhang4Xiaoxia Ding5Jin Mao6Jing Wu7Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaA highly sensitive aptasensor for aflatoxin M1 (AFM1) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method and exhibited broad and strong absorption in the whole ultraviolet-visible (UV-Vis) range. The strong coordination interaction between nitrogen functional groups of the AFM1 aptamer and PdNPs brought FAM and PdNPs in close proximity, which resulted in the fluorescence quenching of FAM to a maximum extent of 95%. The non-specific fluorescence quenching caused by PdNPs towards fluorescein was negligible. After the introduction of AFM1 into the FAM-AFM1 aptamer-PdNPs FRET system, the AFM1 aptamer preferentially combined with AFM1 accompanied by conformational change, which greatly weakened the coordination interaction between the AFM1 aptamer and PdNPs. Thus, fluorescence recovery of FAM was observed and a linear relationship between the fluorescence recovery and the concentration of AFM1 was obtained in the range of 5–150 pg/mL in aqueous buffer with the detection limit of 1.5 pg/mL. AFM1 detection was also realized in milk samples with a linear detection range from 6 pg/mL to 150 pg/mL. The highly sensitive FRET aptasensor with simple configuration shows promising prospect in detecting a variety of food contaminants.https://www.mdpi.com/2072-6651/9/10/318aflatoxin M1palladium nanoparticlesaptasensorfluorescence resonance energy transfer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui Li Daibin Yang Peiwu Li Qi Zhang Wen Zhang Xiaoxia Ding Jin Mao Jing Wu |
spellingShingle |
Hui Li Daibin Yang Peiwu Li Qi Zhang Wen Zhang Xiaoxia Ding Jin Mao Jing Wu Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk Toxins aflatoxin M1 palladium nanoparticles aptasensor fluorescence resonance energy transfer |
author_facet |
Hui Li Daibin Yang Peiwu Li Qi Zhang Wen Zhang Xiaoxia Ding Jin Mao Jing Wu |
author_sort |
Hui Li |
title |
Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk |
title_short |
Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk |
title_full |
Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk |
title_fullStr |
Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk |
title_full_unstemmed |
Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk |
title_sort |
palladium nanoparticles-based fluorescence resonance energy transfer aptasensor for highly sensitive detection of aflatoxin m1 in milk |
publisher |
MDPI AG |
series |
Toxins |
issn |
2072-6651 |
publishDate |
2017-10-01 |
description |
A highly sensitive aptasensor for aflatoxin M1 (AFM1) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method and exhibited broad and strong absorption in the whole ultraviolet-visible (UV-Vis) range. The strong coordination interaction between nitrogen functional groups of the AFM1 aptamer and PdNPs brought FAM and PdNPs in close proximity, which resulted in the fluorescence quenching of FAM to a maximum extent of 95%. The non-specific fluorescence quenching caused by PdNPs towards fluorescein was negligible. After the introduction of AFM1 into the FAM-AFM1 aptamer-PdNPs FRET system, the AFM1 aptamer preferentially combined with AFM1 accompanied by conformational change, which greatly weakened the coordination interaction between the AFM1 aptamer and PdNPs. Thus, fluorescence recovery of FAM was observed and a linear relationship between the fluorescence recovery and the concentration of AFM1 was obtained in the range of 5–150 pg/mL in aqueous buffer with the detection limit of 1.5 pg/mL. AFM1 detection was also realized in milk samples with a linear detection range from 6 pg/mL to 150 pg/mL. The highly sensitive FRET aptasensor with simple configuration shows promising prospect in detecting a variety of food contaminants. |
topic |
aflatoxin M1 palladium nanoparticles aptasensor fluorescence resonance energy transfer |
url |
https://www.mdpi.com/2072-6651/9/10/318 |
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