Anti-Diabetic Activity and Metabolic Changes Induced by Andrographis paniculata Plant Extract in Obese Diabetic Rats
Andrographis paniculata is an annual herb and widely cultivated in Southeast Asian countries for its medicinal use. In recent investigations, A. paniculata was found to be effective against Type 1 diabetes mellitus (Type 1 DM). Here, we used a non-genetic out-bred Sprague-Dawley rat model to test th...
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doaj-7e11362cccd84f4c873c5344e0a11dac2020-11-24T21:04:24ZengMDPI AGMolecules1420-30492016-08-01218102610.3390/molecules21081026molecules21081026Anti-Diabetic Activity and Metabolic Changes Induced by Andrographis paniculata Plant Extract in Obese Diabetic RatsMuhammad Tayyab Akhtar0Mohamad Syakir Bin Mohd Sarib1Intan Safinar Ismail2Faridah Abas3Amin Ismail4Nordin Hj Lajis5Khozirah Shaari6Laboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, Serdang 43400, MalaysiaLaboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, Serdang 43400, MalaysiaLaboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, Serdang 43400, MalaysiaLaboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, Serdang 43400, MalaysiaFaculty of Medicine and Health Sciences, University Putra Malaysia, Serdang 43400, MalaysiaLaboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, Serdang 43400, MalaysiaLaboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, Serdang 43400, MalaysiaAndrographis paniculata is an annual herb and widely cultivated in Southeast Asian countries for its medicinal use. In recent investigations, A. paniculata was found to be effective against Type 1 diabetes mellitus (Type 1 DM). Here, we used a non-genetic out-bred Sprague-Dawley rat model to test the antidiabetic activity of A. paniculata against Type 2 diabetes mellitus (Type 2 DM). Proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy in combination with multivariate data analyses was used to evaluate the A. paniculata and metformin induced metabolic effects on the obese and obese–diabetic (obdb) rat models. Compared to the normal rats, high levels of creatinine, lactate, and allantoin were found in the urine of obese rats, whereas, obese-diabetic rats were marked by high glucose, choline and taurine levels, and low lactate, formate, creatinine, citrate, 2-oxoglutarate, succinate, dimethylamine, acetoacetate, acetate, allantoin and hippurate levels. Treatment of A. paniculata leaf water extract was found to be quite effective in restoring the disturbed metabolic profile of obdb rats back towards normal conditions. Thisstudy shows the anti-diabetic potential of A. paniculata plant extract and strengthens the idea of using this plant against the diabetes. Further classical genetic methods and state of the art molecular techniques could provide insights into the molecular mechanisms involved in the pathogenesis of diabetes mellitus and anti-diabetic effects of A. paniculata water extract.http://www.mdpi.com/1420-3049/21/8/1026Andrographis paniculatametabolomicsdiabetesobese-diabetic rattype 2 DMNMR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Tayyab Akhtar Mohamad Syakir Bin Mohd Sarib Intan Safinar Ismail Faridah Abas Amin Ismail Nordin Hj Lajis Khozirah Shaari |
spellingShingle |
Muhammad Tayyab Akhtar Mohamad Syakir Bin Mohd Sarib Intan Safinar Ismail Faridah Abas Amin Ismail Nordin Hj Lajis Khozirah Shaari Anti-Diabetic Activity and Metabolic Changes Induced by Andrographis paniculata Plant Extract in Obese Diabetic Rats Molecules Andrographis paniculata metabolomics diabetes obese-diabetic rat type 2 DM NMR |
author_facet |
Muhammad Tayyab Akhtar Mohamad Syakir Bin Mohd Sarib Intan Safinar Ismail Faridah Abas Amin Ismail Nordin Hj Lajis Khozirah Shaari |
author_sort |
Muhammad Tayyab Akhtar |
title |
Anti-Diabetic Activity and Metabolic Changes Induced by Andrographis paniculata Plant Extract in Obese Diabetic Rats |
title_short |
Anti-Diabetic Activity and Metabolic Changes Induced by Andrographis paniculata Plant Extract in Obese Diabetic Rats |
title_full |
Anti-Diabetic Activity and Metabolic Changes Induced by Andrographis paniculata Plant Extract in Obese Diabetic Rats |
title_fullStr |
Anti-Diabetic Activity and Metabolic Changes Induced by Andrographis paniculata Plant Extract in Obese Diabetic Rats |
title_full_unstemmed |
Anti-Diabetic Activity and Metabolic Changes Induced by Andrographis paniculata Plant Extract in Obese Diabetic Rats |
title_sort |
anti-diabetic activity and metabolic changes induced by andrographis paniculata plant extract in obese diabetic rats |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2016-08-01 |
description |
Andrographis paniculata is an annual herb and widely cultivated in Southeast Asian countries for its medicinal use. In recent investigations, A. paniculata was found to be effective against Type 1 diabetes mellitus (Type 1 DM). Here, we used a non-genetic out-bred Sprague-Dawley rat model to test the antidiabetic activity of A. paniculata against Type 2 diabetes mellitus (Type 2 DM). Proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy in combination with multivariate data analyses was used to evaluate the A. paniculata and metformin induced metabolic effects on the obese and obese–diabetic (obdb) rat models. Compared to the normal rats, high levels of creatinine, lactate, and allantoin were found in the urine of obese rats, whereas, obese-diabetic rats were marked by high glucose, choline and taurine levels, and low lactate, formate, creatinine, citrate, 2-oxoglutarate, succinate, dimethylamine, acetoacetate, acetate, allantoin and hippurate levels. Treatment of A. paniculata leaf water extract was found to be quite effective in restoring the disturbed metabolic profile of obdb rats back towards normal conditions. Thisstudy shows the anti-diabetic potential of A. paniculata plant extract and strengthens the idea of using this plant against the diabetes. Further classical genetic methods and state of the art molecular techniques could provide insights into the molecular mechanisms involved in the pathogenesis of diabetes mellitus and anti-diabetic effects of A. paniculata water extract. |
topic |
Andrographis paniculata metabolomics diabetes obese-diabetic rat type 2 DM NMR |
url |
http://www.mdpi.com/1420-3049/21/8/1026 |
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