Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae.
BACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. p...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4019481?pdf=render |
id |
doaj-7eb5cff651b84e65bb6baae60c9ac09c |
---|---|
record_format |
Article |
spelling |
doaj-7eb5cff651b84e65bb6baae60c9ac09c2020-11-25T02:12:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9628810.1371/journal.pone.0096288Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae.Vijaya Bharathi SrinivasanBharat Bhushan SinghNitesh PriyadarshiNeeraj Kumar ChauhanGovindan RajamohanBACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. pneumoniae bacillus, apparently less is known about their physiological relevance. PRINCIPAL FINDINGS: Insertional inactivation of kpnGH resulting in increased susceptibility to antibiotics such as azithromycin, ceftazidime, ciprofloxacin, ertapenem, erythromycin, gentamicin, imipenem, ticarcillin, norfloxacin, polymyxin-B, piperacillin, spectinomycin, tobramycin and streptomycin, including dyes and detergents such as ethidium bromide, acriflavine, deoxycholate, sodium dodecyl sulphate, and disinfectants benzalkonium chloride, chlorhexidine and triclosan signifies the wide substrate specificity of the transporter in K. pneumoniae. Growth inactivation and direct fluorimetric efflux assays provide evidence that kpnGH mediates antimicrobial resistance by active extrusion in K. pneumoniae. The kpnGH isogenic mutant displayed decreased tolerance to cell envelope stressors emphasizing its added role in K. pneumoniae physiology. CONCLUSIONS AND SIGNIFICANCE: The MFS efflux pump KpnGH involves in crucial physiological functions besides being an intrinsic resistance determinant in K. pneumoniae.http://europepmc.org/articles/PMC4019481?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vijaya Bharathi Srinivasan Bharat Bhushan Singh Nitesh Priyadarshi Neeraj Kumar Chauhan Govindan Rajamohan |
spellingShingle |
Vijaya Bharathi Srinivasan Bharat Bhushan Singh Nitesh Priyadarshi Neeraj Kumar Chauhan Govindan Rajamohan Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae. PLoS ONE |
author_facet |
Vijaya Bharathi Srinivasan Bharat Bhushan Singh Nitesh Priyadarshi Neeraj Kumar Chauhan Govindan Rajamohan |
author_sort |
Vijaya Bharathi Srinivasan |
title |
Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae. |
title_short |
Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae. |
title_full |
Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae. |
title_fullStr |
Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae. |
title_full_unstemmed |
Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae. |
title_sort |
role of novel multidrug efflux pump involved in drug resistance in klebsiella pneumoniae. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
BACKGROUND: Multidrug resistant Klebsiella pneumoniae have caused major therapeutic problems worldwide due to the emergence of the extended-spectrum β-lactamase producing strains. Although there are >10 major facilitator super family (MFS) efflux pumps annotated in the genome sequence of the K. pneumoniae bacillus, apparently less is known about their physiological relevance. PRINCIPAL FINDINGS: Insertional inactivation of kpnGH resulting in increased susceptibility to antibiotics such as azithromycin, ceftazidime, ciprofloxacin, ertapenem, erythromycin, gentamicin, imipenem, ticarcillin, norfloxacin, polymyxin-B, piperacillin, spectinomycin, tobramycin and streptomycin, including dyes and detergents such as ethidium bromide, acriflavine, deoxycholate, sodium dodecyl sulphate, and disinfectants benzalkonium chloride, chlorhexidine and triclosan signifies the wide substrate specificity of the transporter in K. pneumoniae. Growth inactivation and direct fluorimetric efflux assays provide evidence that kpnGH mediates antimicrobial resistance by active extrusion in K. pneumoniae. The kpnGH isogenic mutant displayed decreased tolerance to cell envelope stressors emphasizing its added role in K. pneumoniae physiology. CONCLUSIONS AND SIGNIFICANCE: The MFS efflux pump KpnGH involves in crucial physiological functions besides being an intrinsic resistance determinant in K. pneumoniae. |
url |
http://europepmc.org/articles/PMC4019481?pdf=render |
work_keys_str_mv |
AT vijayabharathisrinivasan roleofnovelmultidrugeffluxpumpinvolvedindrugresistanceinklebsiellapneumoniae AT bharatbhushansingh roleofnovelmultidrugeffluxpumpinvolvedindrugresistanceinklebsiellapneumoniae AT niteshpriyadarshi roleofnovelmultidrugeffluxpumpinvolvedindrugresistanceinklebsiellapneumoniae AT neerajkumarchauhan roleofnovelmultidrugeffluxpumpinvolvedindrugresistanceinklebsiellapneumoniae AT govindanrajamohan roleofnovelmultidrugeffluxpumpinvolvedindrugresistanceinklebsiellapneumoniae |
_version_ |
1724910630489554944 |