VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES

Presently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed, because sufficient amounts of DNAof some spec...

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Main Authors:	A. G. Bogomolov, K. S. Zadesenets, T. V. Karamysheva, N. L. Podkolodnyi, N. B. Rubtsov
Format:	Article
Language:	English
Published:	Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2014-12-01
Series:	Vavilovskij Žurnal Genetiki i Selekcii
Subjects:	opisthorchis felineus metorchis xanthosomus homo sapiens meiotic chromosomes interspersed repetitive dna chromosome-specific dna sequences fluorescence in situ hybridization (fish) chromosomal in situ suppression hybridization (ciss-hybridization)
Online Access:	https://vavilov.elpub.ru/jour/article/view/51

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spelling	doaj-7ebd0fe27638483c906cf10de4529efb2021-09-11T08:41:13ZengInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesVavilovskij Žurnal Genetiki i Selekcii2500-04622500-32592014-12-0116234835735VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMESA. G. Bogomolov0K. S. Zadesenets1T. V. Karamysheva2N. L. Podkolodnyi3N. B. Rubtsov4Institute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, Russia Institute of Computational Mathematics and Mathematical Geophysics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, RussiaPresently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed, because sufficient amounts of DNAof some species are not available. This article presents a new approach, which allows visualization of a signal from chromosome-specific DNAsequences by means of computer-assisted analysis of FISH images.https://vavilov.elpub.ru/jour/article/view/51opisthorchis felineusmetorchis xanthosomushomo sapiensmeiotic chromosomesinterspersed repetitive dnachromosome-specific dna sequencesfluorescence in situ hybridization (fish)chromosomal in situ suppression hybridization (ciss-hybridization)
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	A. G. Bogomolov K. S. Zadesenets T. V. Karamysheva N. L. Podkolodnyi N. B. Rubtsov
spellingShingle	A. G. Bogomolov K. S. Zadesenets T. V. Karamysheva N. L. Podkolodnyi N. B. Rubtsov VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES Vavilovskij Žurnal Genetiki i Selekcii opisthorchis felineus metorchis xanthosomus homo sapiens meiotic chromosomes interspersed repetitive dna chromosome-specific dna sequences fluorescence in situ hybridization (fish) chromosomal in situ suppression hybridization (ciss-hybridization)
author_facet	A. G. Bogomolov K. S. Zadesenets T. V. Karamysheva N. L. Podkolodnyi N. B. Rubtsov
author_sort	A. G. Bogomolov
title	VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES
title_short	VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES
title_full	VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES
title_fullStr	VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES
title_full_unstemmed	VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES
title_sort	visualization of chromosome-specific dna sequences by fluorescence in situ hybridization of microdissection dna probes with metaphase chromosomes
publisher	Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
series	Vavilovskij Žurnal Genetiki i Selekcii
issn	2500-0462 2500-3259
publishDate	2014-12-01
description	Presently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed, because sufficient amounts of DNAof some species are not available. This article presents a new approach, which allows visualization of a signal from chromosome-specific DNAsequences by means of computer-assisted analysis of FISH images.
topic	opisthorchis felineus metorchis xanthosomus homo sapiens meiotic chromosomes interspersed repetitive dna chromosome-specific dna sequences fluorescence in situ hybridization (fish) chromosomal in situ suppression hybridization (ciss-hybridization)
url	https://vavilov.elpub.ru/jour/article/view/51
work_keys_str_mv	AT agbogomolov visualizationofchromosomespecificdnasequencesbyfluorescenceinsituhybridizationofmicrodissectiondnaprobeswithmetaphasechromosomes AT kszadesenets visualizationofchromosomespecificdnasequencesbyfluorescenceinsituhybridizationofmicrodissectiondnaprobeswithmetaphasechromosomes AT tvkaramysheva visualizationofchromosomespecificdnasequencesbyfluorescenceinsituhybridizationofmicrodissectiondnaprobeswithmetaphasechromosomes AT nlpodkolodnyi visualizationofchromosomespecificdnasequencesbyfluorescenceinsituhybridizationofmicrodissectiondnaprobeswithmetaphasechromosomes AT nbrubtsov visualizationofchromosomespecificdnasequencesbyfluorescenceinsituhybridizationofmicrodissectiondnaprobeswithmetaphasechromosomes
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VISUALIZATION OF CHROMOSOME-SPECIFIC DNA SEQUENCES BY FLUORESCENCE IN SITU HYBRIDIZATION OF MICRODISSECTION DNA PROBES WITH METAPHASE CHROMOSOMES

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