N-acetylglucosamine-mediated expression of nagA and nagB in Streptococcus pneumoniae
In this study, we have explored the transcriptomic response of Streptococcus pneumoniae D39 to N-acetylglucosamine (NAG). Transcriptome comparison of S. pneumoniae D39 wild-type grown in chemically defined medium (CDM) in the presence of 0.5% NAG to that grown in the presence of 0.5% glucose reveale...
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2016-11-01
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doaj-7edb9cb1237a4e54918ea66782bb424a2020-11-24T23:52:08ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882016-11-01610.3389/fcimb.2016.00158185740N-acetylglucosamine-mediated expression of nagA and nagB in Streptococcus pneumoniaeMuhammad Afzal0Muhammad Afzal1Sulman Shafeeq2Irfan Manzoor3Irfan Manzoor4Birgitta Henriques-Normark5Oscar P. Kuipers6University of GroningenGovernment College UniversityKarolinska InstitutetUniversity of GroningenGovernment College UniversityKarolinska InstitutetUniversity of GroningenIn this study, we have explored the transcriptomic response of Streptococcus pneumoniae D39 to N-acetylglucosamine (NAG). Transcriptome comparison of S. pneumoniae D39 wild-type grown in chemically defined medium (CDM) in the presence of 0.5% NAG to that grown in the presence of 0.5% glucose revealed elevated expression of many genes/ operons, including nagA, nagB, manLMN, and nanP. We have further confirmed the NAG-dependent expression of nagA, nagB, manLMN, and nanP by β-galactosidase assays. nagA, nagB and glmS are putatively regulated by a transcriptional regulator NagR. We predicted the operator site of NagR (dre site) in PnagA, PnagB and PglmS, which was further confirmed by mutating the predicted dre site in the respective promoters (nagA, nagB and glmS). Growth comparison of ΔnagA, ΔnagB and ΔglmS with the D39 wild-type demonstrates that nagA and nagB are essential for S. pneumoniae D39 to grow in the presence of NAG as a sole carbon source. Furthermore, deletion of ccpA shows that CcpA has no effect on the expression of nagA, nagB and glmS in the presence of NAG in S. pneumoniae.http://journal.frontiersin.org/Journal/10.3389/fcimb.2016.00158/fullNAGN-acetylglucosamineGLMsCCPANagANagB |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Afzal Muhammad Afzal Sulman Shafeeq Irfan Manzoor Irfan Manzoor Birgitta Henriques-Normark Oscar P. Kuipers |
spellingShingle |
Muhammad Afzal Muhammad Afzal Sulman Shafeeq Irfan Manzoor Irfan Manzoor Birgitta Henriques-Normark Oscar P. Kuipers N-acetylglucosamine-mediated expression of nagA and nagB in Streptococcus pneumoniae Frontiers in Cellular and Infection Microbiology NAG N-acetylglucosamine GLMs CCPA NagA NagB |
author_facet |
Muhammad Afzal Muhammad Afzal Sulman Shafeeq Irfan Manzoor Irfan Manzoor Birgitta Henriques-Normark Oscar P. Kuipers |
author_sort |
Muhammad Afzal |
title |
N-acetylglucosamine-mediated expression of nagA and nagB in Streptococcus pneumoniae |
title_short |
N-acetylglucosamine-mediated expression of nagA and nagB in Streptococcus pneumoniae |
title_full |
N-acetylglucosamine-mediated expression of nagA and nagB in Streptococcus pneumoniae |
title_fullStr |
N-acetylglucosamine-mediated expression of nagA and nagB in Streptococcus pneumoniae |
title_full_unstemmed |
N-acetylglucosamine-mediated expression of nagA and nagB in Streptococcus pneumoniae |
title_sort |
n-acetylglucosamine-mediated expression of naga and nagb in streptococcus pneumoniae |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2016-11-01 |
description |
In this study, we have explored the transcriptomic response of Streptococcus pneumoniae D39 to N-acetylglucosamine (NAG). Transcriptome comparison of S. pneumoniae D39 wild-type grown in chemically defined medium (CDM) in the presence of 0.5% NAG to that grown in the presence of 0.5% glucose revealed elevated expression of many genes/ operons, including nagA, nagB, manLMN, and nanP. We have further confirmed the NAG-dependent expression of nagA, nagB, manLMN, and nanP by β-galactosidase assays. nagA, nagB and glmS are putatively regulated by a transcriptional regulator NagR. We predicted the operator site of NagR (dre site) in PnagA, PnagB and PglmS, which was further confirmed by mutating the predicted dre site in the respective promoters (nagA, nagB and glmS). Growth comparison of ΔnagA, ΔnagB and ΔglmS with the D39 wild-type demonstrates that nagA and nagB are essential for S. pneumoniae D39 to grow in the presence of NAG as a sole carbon source. Furthermore, deletion of ccpA shows that CcpA has no effect on the expression of nagA, nagB and glmS in the presence of NAG in S. pneumoniae. |
topic |
NAG N-acetylglucosamine GLMs CCPA NagA NagB |
url |
http://journal.frontiersin.org/Journal/10.3389/fcimb.2016.00158/full |
work_keys_str_mv |
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