Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens

Chronothanatology has always been a challenge in forensic sciences. Therefore, the importance of a multidisciplinary approach for the characterization of matrices (organs, tissues, or fluids) that respond linearly to the postmortem interval (PMI) is emerging increasingly. The vitreous humor is parti...

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Main Authors: Roberta Risoluti, Giuseppina Gullifa, Vittorio Fineschi, Paola Frati, Stefano Materazzi
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Diagnostics
Subjects:
Online Access:https://www.mdpi.com/2075-4418/11/1/121
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spelling doaj-7f1d5a2dcbce4994b17357647735542c2021-01-15T00:01:10ZengMDPI AGDiagnostics2075-44182021-01-011112112110.3390/diagnostics11010121Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated SpecimensRoberta Risoluti0Giuseppina Gullifa1Vittorio Fineschi2Paola Frati3Stefano Materazzi4Department of Chemistry, “Sapienza” University of Rome, p.le A.Moro 5, 00185 Rome, ItalyDepartment of Chemistry, “Sapienza” University of Rome, p.le A.Moro 5, 00185 Rome, ItalyDepartment of Anatomical, Histological, Forensic Medicine and Orthopaedic Sciences, “Sapienza” University of Rome, p.le A.Moro 5, 00185 Rome, ItalyDepartment of Anatomical, Histological, Forensic Medicine and Orthopaedic Sciences, “Sapienza” University of Rome, p.le A.Moro 5, 00185 Rome, ItalyDepartment of Chemistry, “Sapienza” University of Rome, p.le A.Moro 5, 00185 Rome, ItalyChronothanatology has always been a challenge in forensic sciences. Therefore, the importance of a multidisciplinary approach for the characterization of matrices (organs, tissues, or fluids) that respond linearly to the postmortem interval (PMI) is emerging increasingly. The vitreous humor is particularly suitable for studies aimed at assessing time-related modifications because it is topographically isolated and well-protected. In this work, a novel approach based on thermogravimetry and chemometrics was used to estimate the time since death in the vitreous humor and to collect a databank of samples derived from postmortem examinations after medico–legal evaluation. In this study, contaminated and uncontaminated specimens with tissue fragments were included in order to develop a classification model to predict time of death based on partial least squares discriminant analysis (PLS-DA) that was as robust as possible. Results demonstrate the possibility to correctly predict the PMI even in contaminated samples, with an accuracy not lower than 70%. In addition, the correlation coefficient of the measured versus predicted outcomes was found to be 0.9978, confirming the ability of the model to extend its feasibility even to such situations involving contaminated vitreous humor.https://www.mdpi.com/2075-4418/11/1/121vitreous humorpostmortem intervalthermogravimetrychemometricsforensic pathology
collection DOAJ
language English
format Article
sources DOAJ
author Roberta Risoluti
Giuseppina Gullifa
Vittorio Fineschi
Paola Frati
Stefano Materazzi
spellingShingle Roberta Risoluti
Giuseppina Gullifa
Vittorio Fineschi
Paola Frati
Stefano Materazzi
Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens
Diagnostics
vitreous humor
postmortem interval
thermogravimetry
chemometrics
forensic pathology
author_facet Roberta Risoluti
Giuseppina Gullifa
Vittorio Fineschi
Paola Frati
Stefano Materazzi
author_sort Roberta Risoluti
title Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens
title_short Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens
title_full Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens
title_fullStr Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens
title_full_unstemmed Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens
title_sort application of innovative tga/chemometric approach for forensic purposes: the estimation of the time since death in contaminated specimens
publisher MDPI AG
series Diagnostics
issn 2075-4418
publishDate 2021-01-01
description Chronothanatology has always been a challenge in forensic sciences. Therefore, the importance of a multidisciplinary approach for the characterization of matrices (organs, tissues, or fluids) that respond linearly to the postmortem interval (PMI) is emerging increasingly. The vitreous humor is particularly suitable for studies aimed at assessing time-related modifications because it is topographically isolated and well-protected. In this work, a novel approach based on thermogravimetry and chemometrics was used to estimate the time since death in the vitreous humor and to collect a databank of samples derived from postmortem examinations after medico–legal evaluation. In this study, contaminated and uncontaminated specimens with tissue fragments were included in order to develop a classification model to predict time of death based on partial least squares discriminant analysis (PLS-DA) that was as robust as possible. Results demonstrate the possibility to correctly predict the PMI even in contaminated samples, with an accuracy not lower than 70%. In addition, the correlation coefficient of the measured versus predicted outcomes was found to be 0.9978, confirming the ability of the model to extend its feasibility even to such situations involving contaminated vitreous humor.
topic vitreous humor
postmortem interval
thermogravimetry
chemometrics
forensic pathology
url https://www.mdpi.com/2075-4418/11/1/121
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