Build Your Own Microscope: Step-By-Step Guide for Building a Prism-Based TIRF Microscope

Build Your Own Microscope: Step-By-Step Guide for Building a Prism-Based TIRF Microscope

Prism-based total internal reflection fluorescence (pTIRF) microscopy is one of the most widely used techniques for the single molecule analysis of a vast range of samples including biomolecules, nanostructures, and cells, to name a few. It allows for excitation of surface bound molecules/particles/...

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Bibliographic Details
Main Authors: Dalton R. Gibbs, Anisa Kaur, Anoja Megalathan, Kumar Sapkota, Soma Dhakal
Format: Article
Language:English
Published: MDPI AG 2018-11-01
Series:Methods and Protocols
Subjects:
fluorescence microscope
pTIRF
single-molecule FRET
single-molecule detection
Online Access:https://www.mdpi.com/2409-9279/1/4/40

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https://www.mdpi.com/2409-9279/1/4/40

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