L-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarction
Brief introduction: Myocardial infarction (MI) is a common manifestation of certain cardiac diseases where oxidative stress and fibrosis aggravate the condition markedly. Main objective of the study: Investigation of L-carnitine’s cardioprotective roles and mechanism of action in a rat model of MI....
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doaj-80af481e33f7452eb95a8cbf18f826d22021-10-11T04:14:45ZengElsevierBiomedicine & Pharmacotherapy0753-33222021-11-01143112139L-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarctionTushar Emran0Nowreen Islam Chowdhury1Manoneeta Sarker2Asim Kumar Bepari3Murad Hossain4G.M. Sayedur Rahman5Hasan Mahmud Reza6Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Bashundhara R/A, Dhaka 1229, BangladeshDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Bashundhara R/A, Dhaka 1229, BangladeshDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Bashundhara R/A, Dhaka 1229, BangladeshDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Bashundhara R/A, Dhaka 1229, BangladeshDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Bashundhara R/A, Dhaka 1229, BangladeshDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Bashundhara R/A, Dhaka 1229, BangladeshCorresponding author.; Department of Pharmaceutical Sciences, School of Health and Life Sciences, North South University, Bashundhara R/A, Dhaka 1229, BangladeshBrief introduction: Myocardial infarction (MI) is a common manifestation of certain cardiac diseases where oxidative stress and fibrosis aggravate the condition markedly. Main objective of the study: Investigation of L-carnitine’s cardioprotective roles and mechanism of action in a rat model of MI. Methods: To develop a MI animal model, Isoproterenol (ISO) was administered in male Long Evans rats where animals were divided into five groups (six rats/group). The oxidative stress and antioxidant enzyme activities were determined by different biochemical tests. The real-time PCR was performed to determine the expression of TNF-α and Il-1β. Histopathological observations by hematoxylin-eosin and Masson trichrome were made to observe the tissue damage and fibrosis in heart and kidney. Significant findings from the study: The ISO-treated rats showed increased levels of troponin I and lipid peroxidation and lower antioxidant enzyme activity in heart and kidney tissues. The levels of TNF-α and IL-1β were also increased in ISO-rats. Co-administration of L-carnitine with ISO reversed all these parameters. The elevated levels of uric acid and creatinine kinase and ALP, AST and ALT activities in ISO-rats were also significantly reduced by L-carnitine administration. L-carnitine markedly decreased the infiltration of inflammatory cells and improved the tissue architecture in heart and kidney. Control animals did not show any appreciable response upon L-carnitine administration. Relevant contribution to knowledge: These results suggest that L-carnitine plays a defensive role against cardiac and renal damage in ISO-treated MI rat model via suppressing oxidative stress and increasing antioxidant enzyme functions through inhibition of TNF-α and IL-1β.http://www.sciencedirect.com/science/article/pii/S0753332221009239IsoproterenolL-carnitineOxidative stressInflammationCardiac fibrosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tushar Emran Nowreen Islam Chowdhury Manoneeta Sarker Asim Kumar Bepari Murad Hossain G.M. Sayedur Rahman Hasan Mahmud Reza |
spellingShingle |
Tushar Emran Nowreen Islam Chowdhury Manoneeta Sarker Asim Kumar Bepari Murad Hossain G.M. Sayedur Rahman Hasan Mahmud Reza L-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarction Biomedicine & Pharmacotherapy Isoproterenol L-carnitine Oxidative stress Inflammation Cardiac fibrosis |
author_facet |
Tushar Emran Nowreen Islam Chowdhury Manoneeta Sarker Asim Kumar Bepari Murad Hossain G.M. Sayedur Rahman Hasan Mahmud Reza |
author_sort |
Tushar Emran |
title |
L-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarction |
title_short |
L-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarction |
title_full |
L-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarction |
title_fullStr |
L-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarction |
title_full_unstemmed |
L-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarction |
title_sort |
l-carnitine protects cardiac damage by reducing oxidative stress and inflammatory response via inhibition of tumor necrosis factor-alpha and interleukin-1beta against isoproterenol-induced myocardial infarction |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2021-11-01 |
description |
Brief introduction: Myocardial infarction (MI) is a common manifestation of certain cardiac diseases where oxidative stress and fibrosis aggravate the condition markedly. Main objective of the study: Investigation of L-carnitine’s cardioprotective roles and mechanism of action in a rat model of MI. Methods: To develop a MI animal model, Isoproterenol (ISO) was administered in male Long Evans rats where animals were divided into five groups (six rats/group). The oxidative stress and antioxidant enzyme activities were determined by different biochemical tests. The real-time PCR was performed to determine the expression of TNF-α and Il-1β. Histopathological observations by hematoxylin-eosin and Masson trichrome were made to observe the tissue damage and fibrosis in heart and kidney. Significant findings from the study: The ISO-treated rats showed increased levels of troponin I and lipid peroxidation and lower antioxidant enzyme activity in heart and kidney tissues. The levels of TNF-α and IL-1β were also increased in ISO-rats. Co-administration of L-carnitine with ISO reversed all these parameters. The elevated levels of uric acid and creatinine kinase and ALP, AST and ALT activities in ISO-rats were also significantly reduced by L-carnitine administration. L-carnitine markedly decreased the infiltration of inflammatory cells and improved the tissue architecture in heart and kidney. Control animals did not show any appreciable response upon L-carnitine administration. Relevant contribution to knowledge: These results suggest that L-carnitine plays a defensive role against cardiac and renal damage in ISO-treated MI rat model via suppressing oxidative stress and increasing antioxidant enzyme functions through inhibition of TNF-α and IL-1β. |
topic |
Isoproterenol L-carnitine Oxidative stress Inflammation Cardiac fibrosis |
url |
http://www.sciencedirect.com/science/article/pii/S0753332221009239 |
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