Evaluation of OPEN zinc finger nucleases for direct gene targeting of the ROSA26 locus in mouse embryos.

Evaluation of OPEN zinc finger nucleases for direct gene targeting of the ROSA26 locus in mouse embryos.

Zinc finger nucleases (ZFNs) enable precise genome modification in a variety of organisms and cell types. Commercial ZFNs were reported to enhance gene targeting directly in mouse zygotes, whereas similar approaches using publicly available resources have not yet been described. Here we report preci...

Full description

Bibliographic Details
Main Authors: Mario Hermann, Morgan L Maeder, Kyle Rector, Joseph Ruiz, Burkhard Becher, Kurt Bürki, Cyd Khayter, Adriano Aguzzi, J Keith Joung, Thorsten Buch, Pawel Pelczar
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3435328?pdf=render

Internet

http://europepmc.org/articles/PMC3435328?pdf=render

Similar Items