Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus Spores
Chitosan nanoparticles (CS) and chitosan/pepper tree (Schinus molle) essential oil (CS-EO) bionanocomposites were synthesized by nanoprecipitation method and the in vitro antifungal activity against Aspergillus parasiticus spores was evaluated. The shape and size were evaluated by scanning electron...
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2016-01-01
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Series: | Journal of Nanomaterials |
Online Access: | http://dx.doi.org/10.1155/2016/6060137 |
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doaj-824a9139975249329fe5ebff1704a0d12020-11-24T23:17:04ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292016-01-01201610.1155/2016/60601376060137Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus SporesAna Guadalupe Luque-Alcaraz0Mario Onofre Cortez-Rocha1Carlos Arturo Velázquez-Contreras2Ana Lilian Acosta-Silva3Hisila del Carmen Santacruz-Ortega4Armando Burgos-Hernández5Waldo Manuel Argüelles-Monal6Maribel Plascencia-Jatomea7Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, MexicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, MexicoDepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, MexicoDepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, MexicoDepartamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, MexicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C., 85480 Guaymas, SON, MexicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Bulevar Luis Encinas y Rosales s/n, Colonia Centro, 83000 Hermosillo, SON, MexicoChitosan nanoparticles (CS) and chitosan/pepper tree (Schinus molle) essential oil (CS-EO) bionanocomposites were synthesized by nanoprecipitation method and the in vitro antifungal activity against Aspergillus parasiticus spores was evaluated. The shape and size were evaluated by scanning electron microscopy (SEM) and dynamic light scattering (DLS). The surface charge was determined by assessing the zeta potential and the inclusion of essential oil in bionanocomposites using Fourier transform infrared spectroscopy (FT-IR). The effect on cell viability of the fungus was evaluated using the XTT technique and morphometric analysis by image processing. SEM and DLS analysis indicated that spherical particles with larger diameters for CS-EO biocomposites were observed. Zeta potential values were higher (+11.1 ± 1.60 mV) for CS nanoparticles. Results suggest a chemical interaction between chitosan and pepper tree essential oil. The highest concentration of CS-EO complex caused a larger (40–50%) decrease in A. parasiticus viability. The inclusion of pepper tree oil in CS nanoparticles is a feasible alternative to obtain antifungal biocomposites, where the activity that each compound presents individually is strengthened.http://dx.doi.org/10.1155/2016/6060137 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Guadalupe Luque-Alcaraz Mario Onofre Cortez-Rocha Carlos Arturo Velázquez-Contreras Ana Lilian Acosta-Silva Hisila del Carmen Santacruz-Ortega Armando Burgos-Hernández Waldo Manuel Argüelles-Monal Maribel Plascencia-Jatomea |
spellingShingle |
Ana Guadalupe Luque-Alcaraz Mario Onofre Cortez-Rocha Carlos Arturo Velázquez-Contreras Ana Lilian Acosta-Silva Hisila del Carmen Santacruz-Ortega Armando Burgos-Hernández Waldo Manuel Argüelles-Monal Maribel Plascencia-Jatomea Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus Spores Journal of Nanomaterials |
author_facet |
Ana Guadalupe Luque-Alcaraz Mario Onofre Cortez-Rocha Carlos Arturo Velázquez-Contreras Ana Lilian Acosta-Silva Hisila del Carmen Santacruz-Ortega Armando Burgos-Hernández Waldo Manuel Argüelles-Monal Maribel Plascencia-Jatomea |
author_sort |
Ana Guadalupe Luque-Alcaraz |
title |
Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus Spores |
title_short |
Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus Spores |
title_full |
Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus Spores |
title_fullStr |
Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus Spores |
title_full_unstemmed |
Enhanced Antifungal Effect of Chitosan/Pepper Tree (Schinus molle) Essential Oil Bionanocomposites on the Viability of Aspergillus parasiticus Spores |
title_sort |
enhanced antifungal effect of chitosan/pepper tree (schinus molle) essential oil bionanocomposites on the viability of aspergillus parasiticus spores |
publisher |
Hindawi Limited |
series |
Journal of Nanomaterials |
issn |
1687-4110 1687-4129 |
publishDate |
2016-01-01 |
description |
Chitosan nanoparticles (CS) and chitosan/pepper tree (Schinus molle) essential oil (CS-EO) bionanocomposites were synthesized by nanoprecipitation method and the in vitro antifungal activity against Aspergillus parasiticus spores was evaluated. The shape and size were evaluated by scanning electron microscopy (SEM) and dynamic light scattering (DLS). The surface charge was determined by assessing the zeta potential and the inclusion of essential oil in bionanocomposites using Fourier transform infrared spectroscopy (FT-IR). The effect on cell viability of the fungus was evaluated using the XTT technique and morphometric analysis by image processing. SEM and DLS analysis indicated that spherical particles with larger diameters for CS-EO biocomposites were observed. Zeta potential values were higher (+11.1 ± 1.60 mV) for CS nanoparticles. Results suggest a chemical interaction between chitosan and pepper tree essential oil. The highest concentration of CS-EO complex caused a larger (40–50%) decrease in A. parasiticus viability. The inclusion of pepper tree oil in CS nanoparticles is a feasible alternative to obtain antifungal biocomposites, where the activity that each compound presents individually is strengthened. |
url |
http://dx.doi.org/10.1155/2016/6060137 |
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