Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing
Single pot green synthesis of silver nanoparticles (AgNPs) was successfully carried out using medicinal plant extract of Acalypha wilkesiana via bottom-up approach. Five imperative operational parameters (pH, contact time, concentration, volume ratio and temperature) pivotal to the synthesis of silv...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2019-10-01
|
Series: | Heliyon |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019361778 |
id |
doaj-831d65f1dea94a4c841bb319d267cd87 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adewumi Oluwasogo Dada Folahan Amoo Adekola Fehintoluwa Elizabeth Dada Adunola Tabitha Adelani-Akande Micheal Oluwasesan Bello Chidiogo Rita Okonkwo Adejumoke Abosede Inyinbor Abimbola Peter Oluyori Adeniyi Olayanju Kolawole Oluseyi Ajanaku Charles Oluwaseun Adetunji |
spellingShingle |
Adewumi Oluwasogo Dada Folahan Amoo Adekola Fehintoluwa Elizabeth Dada Adunola Tabitha Adelani-Akande Micheal Oluwasesan Bello Chidiogo Rita Okonkwo Adejumoke Abosede Inyinbor Abimbola Peter Oluyori Adeniyi Olayanju Kolawole Oluseyi Ajanaku Charles Oluwaseun Adetunji Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing Heliyon Analytical chemistry Biotechnology Materials chemistry Organic chemistry Pharmaceutical chemistry Pharmaceutical science |
author_facet |
Adewumi Oluwasogo Dada Folahan Amoo Adekola Fehintoluwa Elizabeth Dada Adunola Tabitha Adelani-Akande Micheal Oluwasesan Bello Chidiogo Rita Okonkwo Adejumoke Abosede Inyinbor Abimbola Peter Oluyori Adeniyi Olayanju Kolawole Oluseyi Ajanaku Charles Oluwaseun Adetunji |
author_sort |
Adewumi Oluwasogo Dada |
title |
Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing |
title_short |
Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing |
title_full |
Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing |
title_fullStr |
Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing |
title_full_unstemmed |
Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing |
title_sort |
silver nanoparticle synthesis by acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2019-10-01 |
description |
Single pot green synthesis of silver nanoparticles (AgNPs) was successfully carried out using medicinal plant extract of Acalypha wilkesiana via bottom-up approach. Five imperative operational parameters (pH, contact time, concentration, volume ratio and temperature) pivotal to the synthesis of silver nanoparticles were investigated. The study showed pH 9, 90 min contact time, 0.001 M Ag+ concentration, volume ratio 1:9 (extract: Ag+ solution), and temperature between 90 – 100 °C were important for the synthesis of Acalypha wilkesiana silver nanoparticles (AW-AgNPs). Phytochemical screening confirmed the presence of saponins, flavonoids, phenols and triterpenes for A. wilkesiana. These phytomolecules served as both capping and stabilizing agent in the green synthesis of silver nanoparticles. AW-AgNPs was characterized by UV-Vis Spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy and Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX). The surface Plasmon resonance (SPR) was observed at 450 nm which is a characteristic absorbance region of AW-AgNPs formation as a result of the collective oscillation of free electron of silver nanoparticles. FTIR Spectroscopy confirmed the presence of functional groups responsible for bioreduction of Ag+. SEM and TEM results confirmed a well dispersed AW-AgNPs of spherical shape. EDX shows the elemental distribution and confirmed AgNPs with a characteristic intense peak at 3.0 keV. AW-AgNPs showed significant inhibition against selected Gram negative and Gram positive prevailing bacteria. AW-AgNPs can therefore be recommended as potential antimicrobial and therapeutic agent against multidrug resistant pathogens. |
topic |
Analytical chemistry Biotechnology Materials chemistry Organic chemistry Pharmaceutical chemistry Pharmaceutical science |
url |
http://www.sciencedirect.com/science/article/pii/S2405844019361778 |
work_keys_str_mv |
AT adewumioluwasogodada silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT folahanamooadekola silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT fehintoluwaelizabethdada silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT adunolatabithaadelaniakande silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT michealoluwasesanbello silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT chidiogoritaokonkwo silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT adejumokeabosedeinyinbor silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT abimbolapeteroluyori silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT adeniyiolayanju silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT kolawoleoluseyiajanaku silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting AT charlesoluwaseunadetunji silvernanoparticlesynthesisbyacalyphawilkesianaextractphytochemicalscreeningcharacterizationinfluenceofoperationalparametersandpreliminaryantibacterialtesting |
_version_ |
1724719060891992064 |
spelling |
doaj-831d65f1dea94a4c841bb319d267cd872020-11-25T02:54:55ZengElsevierHeliyon2405-84402019-10-01510e02517Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testingAdewumi Oluwasogo Dada0Folahan Amoo Adekola1Fehintoluwa Elizabeth Dada2Adunola Tabitha Adelani-Akande3Micheal Oluwasesan Bello4Chidiogo Rita Okonkwo5Adejumoke Abosede Inyinbor6Abimbola Peter Oluyori7Adeniyi Olayanju8Kolawole Oluseyi Ajanaku9Charles Oluwaseun Adetunji10Industrial Chemistry Programme, Nanotechnology Laboratory, Department of Physical Sciences, (Science and Technology Research Cluster), College of Pure and Applied Sciences, Landmark University, P.M.B.1001, Omu-Aran, Kwara State, Nigeria; Corresponding author.Department of Industrial Chemistry, University of Ilorin, P.M.B 1515, Unilorin, NigeriaSchool of Postgraduate Studies, Landmark University, P.M.B.1001, Omu-Aran, Kwara State, NigeriaDepartment of Biological Sciences, Microbiology Programme, Landmark University, Omu-Aran, NigeriaNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Oxford, MS, USA; Department of Applied Chemistry, Federal University Dutsin-Ma, Katsina State, NigeriaIndustrial Chemistry Programme, Nanotechnology Laboratory, Department of Physical Sciences, (Science and Technology Research Cluster), College of Pure and Applied Sciences, Landmark University, P.M.B.1001, Omu-Aran, Kwara State, NigeriaIndustrial Chemistry Programme, Nanotechnology Laboratory, Department of Physical Sciences, (Science and Technology Research Cluster), College of Pure and Applied Sciences, Landmark University, P.M.B.1001, Omu-Aran, Kwara State, NigeriaIndustrial Chemistry Programme, Nanotechnology Laboratory, Department of Physical Sciences, (Science and Technology Research Cluster), College of Pure and Applied Sciences, Landmark University, P.M.B.1001, Omu-Aran, Kwara State, NigeriaDepartment of Agricultural and Biosystems Engineering, College of Engineering, Landmark University, P.M.B.1001, Omu-Aran, Kwara State, NigeriaDepartment of Chemistry, Covenant University, P.M.B. 1023, Ota, Ogun State, NigeriaJawaharlal Nehru University, School of Life Sciences, Laboratory of Genetic Manipulation for Stress Alleviation and Value Addition in Plants, New Delhi, 110067, IndiaSingle pot green synthesis of silver nanoparticles (AgNPs) was successfully carried out using medicinal plant extract of Acalypha wilkesiana via bottom-up approach. Five imperative operational parameters (pH, contact time, concentration, volume ratio and temperature) pivotal to the synthesis of silver nanoparticles were investigated. The study showed pH 9, 90 min contact time, 0.001 M Ag+ concentration, volume ratio 1:9 (extract: Ag+ solution), and temperature between 90 – 100 °C were important for the synthesis of Acalypha wilkesiana silver nanoparticles (AW-AgNPs). Phytochemical screening confirmed the presence of saponins, flavonoids, phenols and triterpenes for A. wilkesiana. These phytomolecules served as both capping and stabilizing agent in the green synthesis of silver nanoparticles. AW-AgNPs was characterized by UV-Vis Spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy and Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX). The surface Plasmon resonance (SPR) was observed at 450 nm which is a characteristic absorbance region of AW-AgNPs formation as a result of the collective oscillation of free electron of silver nanoparticles. FTIR Spectroscopy confirmed the presence of functional groups responsible for bioreduction of Ag+. SEM and TEM results confirmed a well dispersed AW-AgNPs of spherical shape. EDX shows the elemental distribution and confirmed AgNPs with a characteristic intense peak at 3.0 keV. AW-AgNPs showed significant inhibition against selected Gram negative and Gram positive prevailing bacteria. AW-AgNPs can therefore be recommended as potential antimicrobial and therapeutic agent against multidrug resistant pathogens.http://www.sciencedirect.com/science/article/pii/S2405844019361778Analytical chemistryBiotechnologyMaterials chemistryOrganic chemistryPharmaceutical chemistryPharmaceutical science |