Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response
The use of inorganic calcium/phosphate supplemented with biopolymers has drawn lots of attention in bone regenerative medicine. While inflammation is required for bone healing, its exacerbation alters tissue regeneration/implants integration. Inspired by bone composition, a friendly automated spray-...
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doaj-83e7e0a6cba64abfba0b95f21ae8493b2020-11-24T22:52:09ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-11-011911345810.3390/ijms19113458ijms19113458Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory ResponseHassan Rammal0Camille Bour1Marie Dubus2Laura Entz3Léa Aubert4Sophie C. Gangloff5Sandra Audonnet6Nicolae B. Bercu7Fouzia Boulmedais8Cedric Mauprivez9Halima Kerdjoudj10EA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP Santé (FED4231), Université de Reims Champagne Ardenne, Reims 1100, FranceEA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP Santé (FED4231), Université de Reims Champagne Ardenne, Reims 1100, FranceEA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP Santé (FED4231), Université de Reims Champagne Ardenne, Reims 1100, FranceEA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP Santé (FED4231), Université de Reims Champagne Ardenne, Reims 1100, FranceEA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP Santé (FED4231), Université de Reims Champagne Ardenne, Reims 1100, FranceEA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP Santé (FED4231), Université de Reims Champagne Ardenne, Reims 1100, FrancePlateau Technique URCACyt, Université de Reims Champagne Ardenne, Reims 51100, FranceEA 4682, Laboratoire de Recherche en Nanoscience (LRN), Université de Reims Champagne-Ardenne, Reims 51100, FranceUniversité de Strasbourg, CNRS, Institut Charles Sadron, UPR22, 23 rue du Loess, BP 84047, 67034 Strasbourg CEDEX 2, FranceEA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP Santé (FED4231), Université de Reims Champagne Ardenne, Reims 1100, FranceEA 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), SFR CAP Santé (FED4231), Université de Reims Champagne Ardenne, Reims 1100, FranceThe use of inorganic calcium/phosphate supplemented with biopolymers has drawn lots of attention in bone regenerative medicine. While inflammation is required for bone healing, its exacerbation alters tissue regeneration/implants integration. Inspired by bone composition, a friendly automated spray-assisted system was used to build bioactive and osteoinductive calcium phosphate/chitosan/hyaluronic acid substrate (CaP-CHI-HA). Exposing monocytes to CaP-CHI-HA resulted in a secretion of pro-healing VEGF and TGF-β growth factors, TNF-α, MCP-1, IL-6 and IL-8 pro-inflammatory mediators but also IL-10 anti-inflammatory cytokine along with an inflammatory index below 1.5 (versus 2.5 and 7.5 following CaP and LPS stimulation, respectively). Although CD44 hyaluronic acid receptor seems not to be involved in the inflammatory regulation, results suggest a potential role of chemical composition and calcium release from build-up substrates, in affecting the intracellular expression of a calcium-sensing receptor. Herein, our findings indicate a great potential of CaP-CHI-HA in providing required inflammation-healing balance, favorable for bone healing/regeneration.https://www.mdpi.com/1422-0067/19/11/3458bone regenerationinflammationmonocytebone inspired materialcytotoxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hassan Rammal Camille Bour Marie Dubus Laura Entz Léa Aubert Sophie C. Gangloff Sandra Audonnet Nicolae B. Bercu Fouzia Boulmedais Cedric Mauprivez Halima Kerdjoudj |
spellingShingle |
Hassan Rammal Camille Bour Marie Dubus Laura Entz Léa Aubert Sophie C. Gangloff Sandra Audonnet Nicolae B. Bercu Fouzia Boulmedais Cedric Mauprivez Halima Kerdjoudj Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response International Journal of Molecular Sciences bone regeneration inflammation monocyte bone inspired material cytotoxicity |
author_facet |
Hassan Rammal Camille Bour Marie Dubus Laura Entz Léa Aubert Sophie C. Gangloff Sandra Audonnet Nicolae B. Bercu Fouzia Boulmedais Cedric Mauprivez Halima Kerdjoudj |
author_sort |
Hassan Rammal |
title |
Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response |
title_short |
Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response |
title_full |
Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response |
title_fullStr |
Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response |
title_full_unstemmed |
Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response |
title_sort |
combining calcium phosphates with polysaccharides: a bone-inspired material modulating monocyte/macrophage early inflammatory response |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-11-01 |
description |
The use of inorganic calcium/phosphate supplemented with biopolymers has drawn lots of attention in bone regenerative medicine. While inflammation is required for bone healing, its exacerbation alters tissue regeneration/implants integration. Inspired by bone composition, a friendly automated spray-assisted system was used to build bioactive and osteoinductive calcium phosphate/chitosan/hyaluronic acid substrate (CaP-CHI-HA). Exposing monocytes to CaP-CHI-HA resulted in a secretion of pro-healing VEGF and TGF-β growth factors, TNF-α, MCP-1, IL-6 and IL-8 pro-inflammatory mediators but also IL-10 anti-inflammatory cytokine along with an inflammatory index below 1.5 (versus 2.5 and 7.5 following CaP and LPS stimulation, respectively). Although CD44 hyaluronic acid receptor seems not to be involved in the inflammatory regulation, results suggest a potential role of chemical composition and calcium release from build-up substrates, in affecting the intracellular expression of a calcium-sensing receptor. Herein, our findings indicate a great potential of CaP-CHI-HA in providing required inflammation-healing balance, favorable for bone healing/regeneration. |
topic |
bone regeneration inflammation monocyte bone inspired material cytotoxicity |
url |
https://www.mdpi.com/1422-0067/19/11/3458 |
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