Performance of the municipal wastewater treatment plant for removal of Listeria monocytogenes

Aims: The aim of present study was determination of occurrence of Listeria Listeria spp. in various point of a municipal wastewater treatment plant. Materials and Methods: The samples were collected of influent, effluent, raw sludge, stabilized sludge and dried sludge from north wastewater treatmen...

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Main Authors: Nahid Navidjouy, Mohammad Jalali, Hossein Movahedian Attar, Hajar Aghili
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2013-01-01
Series:International Journal of Environmental Health Engineering
Subjects:
PCR
Online Access:http://www.ijehe.org/article.asp?issn=2277-9183;year=2013;volume=2;issue=1;spage=27;epage=27;aulast=Navidjouy
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spelling doaj-84714a12a7bc4b57971c4c0edcfd45982020-11-25T00:27:17ZengWolters Kluwer Medknow PublicationsInternational Journal of Environmental Health Engineering 2277-91832013-01-0121272710.4103/2277-9183.113221Performance of the municipal wastewater treatment plant for removal of Listeria monocytogenesNahid NavidjouyMohammad JalaliHossein Movahedian AttarHajar AghiliAims: The aim of present study was determination of occurrence of Listeria Listeria spp. in various point of a municipal wastewater treatment plant. Materials and Methods: The samples were collected of influent, effluent, raw sludge, stabilized sludge and dried sludge from north wastewater treatment plant Isfahan, Iran. The presence of Listeria spp. was determined using USDA procedure and enumerated by a three-tube most probable number assay using Fraser enrichment broth. Then, biochemically identified Listeria monocytogenes was further confirmed by PCR amplification. Results: L. monocytogenes, L. innocua and L. seeligeri were isolated from 76.9%, 23.1% and 23.1% of influent, 38.5%, 46.2% and 7.7% of effluent, 84.6%, 69.2% and 46.2% of raw sludge, 69.2%, 76.9% and 0% of stabilized sludge and 46.2%, 7.7% and 0% of dried sludge samples, respectively. The efficiency of wastewater treatment processes, digester tank and drying bed in removal of L. monocytogenes were 69.6%, 64.7% and 73.4%, respectively. All phenotypically identified L. monocytogenes were further confirmed by PCR method. Conclusion: Application of sewage sludge in agricultural farms as fertilizer may result in bacteria spreading in agriculture fields and contaminated foods with plant origin. This may cause a risk of spreading disease to human and animals. Using parameters such as BOD 5 is not sufficient standard for the elimination of pathogenic microorganisms.http://www.ijehe.org/article.asp?issn=2277-9183;year=2013;volume=2;issue=1;spage=27;epage=27;aulast=NavidjouyEffluentListeria monocytogenesPCRsludgewastewater treatment plant
collection DOAJ
language English
format Article
sources DOAJ
author Nahid Navidjouy
Mohammad Jalali
Hossein Movahedian Attar
Hajar Aghili
spellingShingle Nahid Navidjouy
Mohammad Jalali
Hossein Movahedian Attar
Hajar Aghili
Performance of the municipal wastewater treatment plant for removal of Listeria monocytogenes
International Journal of Environmental Health Engineering
Effluent
Listeria monocytogenes
PCR
sludge
wastewater treatment plant
author_facet Nahid Navidjouy
Mohammad Jalali
Hossein Movahedian Attar
Hajar Aghili
author_sort Nahid Navidjouy
title Performance of the municipal wastewater treatment plant for removal of Listeria monocytogenes
title_short Performance of the municipal wastewater treatment plant for removal of Listeria monocytogenes
title_full Performance of the municipal wastewater treatment plant for removal of Listeria monocytogenes
title_fullStr Performance of the municipal wastewater treatment plant for removal of Listeria monocytogenes
title_full_unstemmed Performance of the municipal wastewater treatment plant for removal of Listeria monocytogenes
title_sort performance of the municipal wastewater treatment plant for removal of listeria monocytogenes
publisher Wolters Kluwer Medknow Publications
series International Journal of Environmental Health Engineering
issn 2277-9183
publishDate 2013-01-01
description Aims: The aim of present study was determination of occurrence of Listeria Listeria spp. in various point of a municipal wastewater treatment plant. Materials and Methods: The samples were collected of influent, effluent, raw sludge, stabilized sludge and dried sludge from north wastewater treatment plant Isfahan, Iran. The presence of Listeria spp. was determined using USDA procedure and enumerated by a three-tube most probable number assay using Fraser enrichment broth. Then, biochemically identified Listeria monocytogenes was further confirmed by PCR amplification. Results: L. monocytogenes, L. innocua and L. seeligeri were isolated from 76.9%, 23.1% and 23.1% of influent, 38.5%, 46.2% and 7.7% of effluent, 84.6%, 69.2% and 46.2% of raw sludge, 69.2%, 76.9% and 0% of stabilized sludge and 46.2%, 7.7% and 0% of dried sludge samples, respectively. The efficiency of wastewater treatment processes, digester tank and drying bed in removal of L. monocytogenes were 69.6%, 64.7% and 73.4%, respectively. All phenotypically identified L. monocytogenes were further confirmed by PCR method. Conclusion: Application of sewage sludge in agricultural farms as fertilizer may result in bacteria spreading in agriculture fields and contaminated foods with plant origin. This may cause a risk of spreading disease to human and animals. Using parameters such as BOD 5 is not sufficient standard for the elimination of pathogenic microorganisms.
topic Effluent
Listeria monocytogenes
PCR
sludge
wastewater treatment plant
url http://www.ijehe.org/article.asp?issn=2277-9183;year=2013;volume=2;issue=1;spage=27;epage=27;aulast=Navidjouy
work_keys_str_mv AT nahidnavidjouy performanceofthemunicipalwastewatertreatmentplantforremovaloflisteriamonocytogenes
AT mohammadjalali performanceofthemunicipalwastewatertreatmentplantforremovaloflisteriamonocytogenes
AT hosseinmovahedianattar performanceofthemunicipalwastewatertreatmentplantforremovaloflisteriamonocytogenes
AT hajaraghili performanceofthemunicipalwastewatertreatmentplantforremovaloflisteriamonocytogenes
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