Short communication: Molecular detection of honeybee viruses in Ecuador
Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural...
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
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doaj-85254de5b4ae4664bd668f788884a0f32020-11-25T03:49:50ZengInstituto Nacional de Investigación y Tecnología Agraria y AlimentariaSpanish Journal of Agricultural Research2171-92922020-04-01181e05SC02e05SC0210.5424/sjar/2020181-157792966Short communication: Molecular detection of honeybee viruses in EcuadorMaría E. Bravi0Jorge Avalos1Hugo Rosero2Gerald Maldonado3Francisco J. Reynaldi4María L. Genchi-García5Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias, Laboratorio de Virología (LAVIR), Calle 60 y 118 s/n, 1900 La Plata (Buenos Aires) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Programa Nacional Sanitario Apícola, AGROCALIDAD, Av. Amazonas y Eloy Alfara, QuitoPrograma Nacional Sanitario Apícola, AGROCALIDAD, Av. Amazonas y Eloy Alfara, QuitoPrograma Nacional Sanitario Apícola, AGROCALIDAD, Av. Amazonas y Eloy Alfara, QuitoUniversidad Nacional de La Plata, Facultad de Ciencias Veterinarias, Laboratorio de Virología (LAVIR), Calle 60 y 118 s/n, 1900 La Plata (Buenos Aires) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias, Laboratorio de Virología (LAVIR), Calle 60 y 118 s/n, 1900 La Plata (Buenos Aires) Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA)Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural production, use of pesticides and presence of pathogens. Viruses, in particular, are suspected to be drivers of colony mortality. In this scenario, the aim of this study was to determine the presence of honeybee viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV) in A. mellifera populations using a RT-mPCR assay. Area of study: Apiaries were situated in Pichincha, Ecuador. Material and methods: Samples were collected from seventeen apiaries that exhibited mortality but without specific clinical signs. Each sample comprised 15 individuals. After RNA extraction, a multiplex PCR analysis was performed for presence of six viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV). Main results: Four of the viruses (ABPV, DWV, BQCV and SBV) were found in co-infections in these colonies, with ABPV and SBV also being found in simple infections. Research highlights: To our knowledge, this is the first molecular detection of BQCV and SBV in Ecuador. These findings suggest that some of the above viruses could be involved in weakening these colonies.https://revistas.inia.es/index.php/sjar/article/view/15779abpv, dwv, sbv, bqcv, multiplex-pcr, apis mellifera |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María E. Bravi Jorge Avalos Hugo Rosero Gerald Maldonado Francisco J. Reynaldi María L. Genchi-García |
spellingShingle |
María E. Bravi Jorge Avalos Hugo Rosero Gerald Maldonado Francisco J. Reynaldi María L. Genchi-García Short communication: Molecular detection of honeybee viruses in Ecuador Spanish Journal of Agricultural Research abpv, dwv, sbv, bqcv, multiplex-pcr, apis mellifera |
author_facet |
María E. Bravi Jorge Avalos Hugo Rosero Gerald Maldonado Francisco J. Reynaldi María L. Genchi-García |
author_sort |
María E. Bravi |
title |
Short communication: Molecular detection of honeybee viruses in Ecuador |
title_short |
Short communication: Molecular detection of honeybee viruses in Ecuador |
title_full |
Short communication: Molecular detection of honeybee viruses in Ecuador |
title_fullStr |
Short communication: Molecular detection of honeybee viruses in Ecuador |
title_full_unstemmed |
Short communication: Molecular detection of honeybee viruses in Ecuador |
title_sort |
short communication: molecular detection of honeybee viruses in ecuador |
publisher |
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria |
series |
Spanish Journal of Agricultural Research |
issn |
2171-9292 |
publishDate |
2020-04-01 |
description |
Aim of study: The honeybee, Apis mellifera, is one of the most important pollinators in the world. Apicultural activity and pollination services have been affected by the decline in the honeybee population, which may be due to the interaction of multiple risk factors, such as changes in agricultural production, use of pesticides and presence of pathogens. Viruses, in particular, are suspected to be drivers of colony mortality. In this scenario, the aim of this study was to determine the presence of honeybee viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV) in A. mellifera populations using a RT-mPCR assay.
Area of study: Apiaries were situated in Pichincha, Ecuador.
Material and methods: Samples were collected from seventeen apiaries that exhibited mortality but without specific clinical signs. Each sample comprised 15 individuals. After RNA extraction, a multiplex PCR analysis was performed for presence of six viruses (IAPV, DWV, SBV, ABPV, BQCV, CBPV).
Main results: Four of the viruses (ABPV, DWV, BQCV and SBV) were found in co-infections in these colonies, with ABPV and SBV also being found in simple infections.
Research highlights: To our knowledge, this is the first molecular detection of BQCV and SBV in Ecuador. These findings suggest that some of the above viruses could be involved in weakening these colonies. |
topic |
abpv, dwv, sbv, bqcv, multiplex-pcr, apis mellifera |
url |
https://revistas.inia.es/index.php/sjar/article/view/15779 |
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