POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS

Diabetes mellitus is a multifactorial disease. It has recently been shown that an insertion (I)/deletion (D) polymorphism exists in the angiotensin-converting enzyme (ACE) gene that can affect the serum ACE level. There are three genotypes: DD, DI, and II, with the ACE level being highest in DD, int...

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Main Authors: A Nikzamir, M Nakhjavani, T Golmohammadi, L Dibai, R Saffary
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2008-08-01
Series:Acta Medica Iranica
Online Access:https://acta.tums.ac.ir/index.php/acta/article/view/3482
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spelling doaj-86f284cc8bf84be6af77ff37ebf2b6a52020-11-25T03:26:31ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942008-08-01464POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTSA Nikzamir0M Nakhjavani1T Golmohammadi2L Dibai3R Saffary4Department of Biochemistry, School of Medicine, Ahwaz Jondi Shapour University of Medical Sciences, Endocrinology and Metabolism Research Center (EMRC), Vali-e-Asr Hospital, Tehran University of MedicDepartment of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Endocrinology and Metabolism Research Center (EMRC), Vali-e-Asr Hospital, Tehran University of MedicEndocrinology and Metabolism Research Center (EMRC), Vali-e-Asr Hospital, Tehran University of MedicDiabetes mellitus is a multifactorial disease. It has recently been shown that an insertion (I)/deletion (D) polymorphism exists in the angiotensin-converting enzyme (ACE) gene that can affect the serum ACE level. There are three genotypes: DD, DI, and II, with the ACE level being highest in DD, intermediate in DI, and lowest in II. In the present investigation, 170 patients with type 2 diabetes mellitus (T2DM) and 144 control subjects were studied. The ACE I/D polymorphism was determined by polymerase chain reaction (PCR) utilizing specific primers. ACE activity was determined spectrophotometrically. Distribution of ACE gene (I/D) polymorphism and allele frequencies in patients with T2DM were significantly different from those in control (P < 0.001); D allele frequency was 51% in T2DM vs. 48% in controls. The level of ACE activity was significantly higher in the DD genotype (91.1 ± 23.18) than those in ID (60.6 ± 22.8) and in II genotypes (36.8 ± 6.9). There was a significant difference in genotype distribution between the two groups (P < 0.001). New normal ranges of serum ACE level were determined for each genotype. Moreover, we found test sensitivity to be 62.3%. Serum ACE activity was significantly associated with ACE (I/D) gene polymorphism. https://acta.tums.ac.ir/index.php/acta/article/view/3482
collection DOAJ
language English
format Article
sources DOAJ
author A Nikzamir
M Nakhjavani
T Golmohammadi
L Dibai
R Saffary
spellingShingle A Nikzamir
M Nakhjavani
T Golmohammadi
L Dibai
R Saffary
POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS
Acta Medica Iranica
author_facet A Nikzamir
M Nakhjavani
T Golmohammadi
L Dibai
R Saffary
author_sort A Nikzamir
title POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS
title_short POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS
title_full POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS
title_fullStr POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS
title_full_unstemmed POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS
title_sort polymorphism in the angiotensin-converting enzyme (ace) gene and ace activity in type 2 diabetic patients
publisher Tehran University of Medical Sciences
series Acta Medica Iranica
issn 0044-6025
1735-9694
publishDate 2008-08-01
description Diabetes mellitus is a multifactorial disease. It has recently been shown that an insertion (I)/deletion (D) polymorphism exists in the angiotensin-converting enzyme (ACE) gene that can affect the serum ACE level. There are three genotypes: DD, DI, and II, with the ACE level being highest in DD, intermediate in DI, and lowest in II. In the present investigation, 170 patients with type 2 diabetes mellitus (T2DM) and 144 control subjects were studied. The ACE I/D polymorphism was determined by polymerase chain reaction (PCR) utilizing specific primers. ACE activity was determined spectrophotometrically. Distribution of ACE gene (I/D) polymorphism and allele frequencies in patients with T2DM were significantly different from those in control (P < 0.001); D allele frequency was 51% in T2DM vs. 48% in controls. The level of ACE activity was significantly higher in the DD genotype (91.1 ± 23.18) than those in ID (60.6 ± 22.8) and in II genotypes (36.8 ± 6.9). There was a significant difference in genotype distribution between the two groups (P < 0.001). New normal ranges of serum ACE level were determined for each genotype. Moreover, we found test sensitivity to be 62.3%. Serum ACE activity was significantly associated with ACE (I/D) gene polymorphism.
url https://acta.tums.ac.ir/index.php/acta/article/view/3482
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AT mnakhjavani polymorphismintheangiotensinconvertingenzymeacegeneandaceactivityintype2diabeticpatients
AT tgolmohammadi polymorphismintheangiotensinconvertingenzymeacegeneandaceactivityintype2diabeticpatients
AT ldibai polymorphismintheangiotensinconvertingenzymeacegeneandaceactivityintype2diabeticpatients
AT rsaffary polymorphismintheangiotensinconvertingenzymeacegeneandaceactivityintype2diabeticpatients
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